HA-tag antibody - 245 011

The HA-Tag is a widely used epitope tag

Mouse monoclonal purified IgG

Cat. No.: 245 011

Amount: 200 µg

Price: $415.00

Cat. No. 245 011	200 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 200 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (see remarks) IP: yes ICC: 1 : 500 gallery IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	12CA5
Subtype	IgG2b (κ light chain)
Immunogen	Synthetic peptide (YPYDVPDYA) corresponding to residues AA 98-106 from human hemaglutinin
Remarks	WB: This antibody tends to produce unspecific background bands around 90-100 kDa. Therefore, we recommend 245 003 for westernblot experiments.
Data sheet	245_011.pdf

Cat. No.: 245 011

Amount: 200 µg

Price: $415.00

ICC

Background

The surface glycoprotein hemaglutinin (HA) of the human influenza virus is essential for the infectivity of the virus. The HA-tag corresponds to amino acids 98-106 of this protein and has been widely used as an epitope tag in protein expression vectors. It can be employed for the detection and immunoisolation of proteins using immunoblotting, immunoprecipitation and immunostaining methods and has been shown to have only neglectable influence on the biological properties of the tagged protein.

1 ) Xie LY et al., Endocrinology (1998)
2 ) Lauber J et al., EMBO J. (1996)
3 ) Howard M et al., Am. J. Physiol. (1995)
4 ) Handley-Gearhart PM et al., J. Biol. Chem. (1994)
5 ) Chen YT et al., Proc. Natl. Acad. Sci. U.S.A. (1993)

Protocols

Standard protocol

ICC

Other products for this target