Cat. No.: 245 003CpH
Amount: 50 µg
Price:
$485.00
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pH-sensitive probe fluorescent only under acidic conditions
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| Cat. No. 245 003CpH |
50 µg specific antibody, lyophilized. Affinity purified with the immunogen, fluorescence-labeled with CypHer5E. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye. |
| Applications | |
| Label | CypHer5E |
| Immunogen | Synthetic peptide corresponding to AA 98 to 108 from human HA-tag |
| Specificity | Specific for HA-tag, hemaglutinin. |
| Matching control protein/peptide | 245-0P |
| Remarks |
This antibody is suitable for internalization studies when GFP is fused to an epitope residing in the lumen of an acidic compartment. The pH sensitive dye regaines its fluorescence after the acidification of the targeted cellular compartment. |
| Data sheet | 245_003cph.pdf |
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