Cat. No.: 245 003CpH
Amount: 50 µg
Price:
$480.00
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pH-sensitive probe fluorescent only under acidic conditions
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| Cat. No. 245 003CpH |
50 µg specific antibody, lyophilized. Affinity purified with the immunogen, fluorescence-labeled with CypHer5E.
CypHer5E® is a pH-sensitive dye, fluorescent only after antibody internalization at the acidic pH of the lumen of the synaptic vesicle. CypHer5E® can be detected with the filter sets for Cy5®. This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Application Number US 2006-0051758 and related patents. Cy, CyDye, and CypHer are trademarks of GE-Healthcare Ltd. Purchase of reagents related to the CypHer technology from Synaptic Systems GmbH provides a license for non-profit and in-house research use only. Any application of above mentioned technology for commercial purpose requires a separate license from GE-Healthcare Bio-Science Corp., 800 Centennial Avenue, Piscataway, NJ 08855-1327, USA. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye. |
| Applications |
IP: N/A ICC: 1 : 200 up to 1 : 500 gallery IHC: not tested yet IHC-P: not tested yet |
| Label | CypHer5E |
| Immunogen | Synthetic peptide corresponding to AA 98 to 108 from human HA-tag |
| Specificity | Specific for HA-tag, hemaglutinin. |
| Matching control protein/peptide | 245-0P |
| Remarks |
This antibody is suitable for internalization studies when GFP is fused to an epitope residing in the lumen of an acidic compartment. The pH sensitive dye regaines its fluorescence after the acidification of the targeted cellular compartment. |
| Data sheet | 245_003cph.pdf |
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