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Home > Products > 245 003CpH

HA-tag antibody - 245 003CpH

The HA-Tag is a widely used epitope tag

pH-sensitive probe fluorescent only under acidic conditions

Rabbit polyclonal purified antibody
Cat. No.: 245 003CpH
Amount: 50 µg
Price: $480.00
Cat. No. 245 003CpH 50 µg specific antibody, lyophilized. Affinity purified with the immunogen, fluorescence-labeled with CypHer5E.

CypHer5E® is a pH-sensitive dye, fluorescent only after antibody internalization at the acidic pH of the lumen of the synaptic vesicle. CypHer5E® can be detected with the filter sets for Cy5®.

CypHer5E®: λex 647 nm / λem 667 nm

This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Application Number US 2006-0051758 and related patents. Cy, CyDye, and CypHer are trademarks of GE-Healthcare Ltd. Purchase of reagents related to the CypHer technology from Synaptic Systems GmbH provides a license for non-profit and in-house research use only. Any application of above mentioned technology for commercial purpose requires a separate license from GE-Healthcare Bio-Science Corp., 800 Centennial Avenue, Piscataway, NJ 08855-1327, USA.
Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Applications
 
WB: N/A
IP: N/A
ICC: 1 : 200 up to 1 : 500 gallery  
IHC: not tested yet
IHC-P: not tested yet

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Label CypHer5E
Immunogen Synthetic peptide corresponding to AA 98 to 108 from human HA-tag
Specificity Specific for HA-tag, hemaglutinin.
Matching control protein/peptide 245-0P
Remarks

This antibody is suitable for internalization studies when GFP is fused to an epitope residing in the lumen of an acidic compartment. The pH sensitive dye regaines its fluorescence after the acidification of the targeted cellular compartment.
Data sheet 245_003cph.pdf
Cat. No.: 245 003CpH
Amount: 50 µg
Price: $480.00
ICC
Background
The surface glycoprotein hemaglutinin (HA) of the human influenza virus is essential for the infectivity of the virus. The HA-tag corresponds to amino acids 98-106 of this protein and has been widely used as an epitope tag in protein expression vectors. It can be employed for the detection and immunoisolation of proteins using immunoblotting, immunoprecipitation and immunostaining methods and has been shown to have only neglectable influence on the biological properties of the tagged protein.
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