SynCAM4 antibody - 243 403

SynCAMs are synaptic adhesion molecules of the Ig superfamily

Rabbit polyclonal purified antibody

Cat. No.: 243 403

Amount: 50 µg

Price: $375.00

Cat. No. 243 403	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: 1 : 100 up to 1 : 500 gallery IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 376 to 388 from mouse SynCAM4 (UniProt Id: Q8R464)
Reactivity	Reacts with: rat (Q1WIM1), mouse (Q8R464). Other species not tested yet.
Matching control protein/peptide	243-4P
Data sheet	243_403.pdf

Cat. No.: 243 403

Amount: 50 µg

Price: $375.00

ICC

Background

Development of synapses involves transsynaptic interactions of dedicated synaptic adhesion molecules like neuroligins, neurexins and synCAMs (Synaptic cell adhesion molecules). Four SynCAM isoforms have been described so far. All share a common domain structure and contain three extracellular Ig domains, a single transmembrane region, and a short COOH-terminal cytoplasmic tail.
SynCAM 2 has been shown to be associated to myelinated axons and synCAM 3 can serve as a receptor for herpes viruses.

1 ) Nagata M et al., Int. J. Cancer (2012)
2 ) Hunter PR et al., J. Comp. Neurol. (2011)
3 ) Raveh S et al., J. Cell. Biochem. (2009)
4 ) Pellissier F et al., BMC Neurosci (2007)
5 ) Maurel P et al., J. Cell Biol. (2007)
6 ) Dong X et al., J. Biol. Chem. (2006)
7 ) Kakunaga S et al., J. Cell. Sci. (2005)
8 ) Fogel AI et al., J. Neurosci. (2007)
9 ) Biederer T et al., Science (2002)

Protocols

ICC

OTHER

Preabsorbtion

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