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SynCAM2 antibody - 243 203 K.O.

SynCAMs are synaptic adhesion molecules of the Ig superfamily
Rabbit polyclonal purified antibody
Cat. No.: 243 203
Amount: 50 µg
Price: $375.00
Cat. No. 243 203 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 100 gallery  
IHC: 1 : 100 gallery  
IHC-P: not tested yet

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.

Immunogen Synthetic peptide corresponding to AA 167 to 181 from mouse SynCAM2 (UniProt Id: Q8BLQ9)
Reactivity Reacts with: rat (Q1WIM2), mouse (Q8BLQ9), human (Q8N3J6).
Other species not tested yet.
Specificity K.O. validated PubMed: 29217450
Matching control protein/peptide 243-2P
Data sheet 243_203.pdf

References for SynCAM2 - 243 203

Cadm2 regulates body weight and energy homeostasis in mice.
Yan X, Wang Z, Schmidt V, Gauert A, Willnow TE, Heinig M, Poy MN
Molecular metabolism (2018) 8: 180-188. 243 203 WB, ICC; KO verified; tested species: mouse
Cadm2 regulates body weight and energy homeostasis in mice.
Yan X, Wang Z, Schmidt V, Gauert A, Willnow TE, Heinig M, Poy MN
Molecular metabolism (2018) 8: 180-188. 243 203 WB, ICC; KO verified; tested species: mouse
Regulation of body weight and energy homeostasis by neuronal cell adhesion molecule 1.
Rathjen T, Yan X, Kononenko NL, Ku MC, Song K, Ferrarese L, Tarallo V, Puchkov D, Kochlamazashvili G, Brachs S, Varela L, et al.
Nature neuroscience (2017) 208: 1096-1103. 243 203 ICC; KO verified; tested species: mouse
Cat. No.: 243 203
Amount: 50 µg
Price: $375.00
Cadm2 regulates body weight and energy homeostasis in mice.
Yan X, Wang Z, Schmidt V, Gauert A, Willnow TE, Heinig M, Poy MN
Molecular metabolism (2018) 8: 180-188. 243 203 WB, ICC; KO verified; tested species: mouse
Cadm2 regulates body weight and energy homeostasis in mice.
Yan X, Wang Z, Schmidt V, Gauert A, Willnow TE, Heinig M, Poy MN
Molecular metabolism (2018) 8: 180-188. 243 203 WB, ICC; KO verified; tested species: mouse
Regulation of body weight and energy homeostasis by neuronal cell adhesion molecule 1.
Rathjen T, Yan X, Kononenko NL, Ku MC, Song K, Ferrarese L, Tarallo V, Puchkov D, Kochlamazashvili G, Brachs S, Varela L, et al.
Nature neuroscience (2017) 208: 1096-1103. 243 203 ICC; KO verified; tested species: mouse
Background

Development of synapses involves transsynaptic interactions of dedicated synaptic adhesion molecules like neuroligins, neurexins and synCAMs (Synaptic cell adhesion molecules). Four SynCAM isoforms have been described so far. All share a common domain structure and contain three extracellular Ig domains, a single transmembrane region, and a short COOH-terminal cytoplasmic tail.
SynCAM 2 has been shown to be associated to myelinated axons and synCAM 3 can serve as a receptor for herpes viruses.