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Home > Products > 243 003

SynCAM1/2/3 antibody - 243 003

SynCAMs are synaptic adhesion molecules of the Ig superfamily
Rabbit polyclonal purified antibody
Cat. No.: 243 003
Amount: 50 µg
Price: $375.00
Cat. No. 243 003 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 100 gallery  
IHC: 1 : 100 up to 1 : 500 gallery  
IHC-P: not tested yet

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen Synthetic peptide corresponding to AA 436 to 446 from mouse SynCAM1 (UniProt Id: Q8R5M8)
Reactivity Reacts with: rat (Q6AYP5, Q1WIM2, Q1WIM3), mouse (Q8R5M8, Q8BLQ9, Q99N28).
Other species not tested yet.
Specificity Recognizes synCAM 1, 2, 3 but not 4.
Matching control protein/peptide 243-0P
Data sheet 243_003.pdf

References for SynCAM1/2/3 - 243 003

Altered expression of synaptic proteins and adhesion molecules in the hippocampus and cortex following the onset of diabetes in nonobese diabetic mice.
Yokokawa T, Kido K, Sato K, Hayashi T, Fujita S
Physiological reports (2023) 118: e15673. 243 003 WB; tested species: mouse
Haploinsufficiency of the Attention-Deficit/Hyperactivity Disorder Risk Gene St3gal3 in Mice Causes Alterations in Cognition and Expression of Genes Involved in Myelination and Sialylation.
Rivero O, Alhama-Riba J, Ku HP, Fischer M, Ortega G, Álmos P, Diouf D, van den Hove D, Lesch KP
Frontiers in genetics (2021) 12: 688488. 243 003 WB; tested species: mouse
A novel synaptic junction preparation for the identification and characterization of cleft proteins.
Burch A, Tao-Cheng JH, Dosemeci A
PloS one (2017) 123: e0174895. 243 003 WB, EM; tested species: rat
Molecular profiling of synaptic vesicle docking sites reveals novel proteins but few differences between glutamatergic and GABAergic synapses.
Boyken J, Grønborg M, Riedel D, Urlaub H, Jahn R, Chua JJ
Neuron (2013) 782: 285-97. 243 003 WB
A High-Resolution Method for Quantitative Molecular Analysis of Functionally Characterized Individual Synapses.
Holderith N, Heredi J, Kis V, Nusser Z
Cell reports (2020) 324: 107968. 243 003 IHC; tested species: rat
A novel synaptic junction preparation for the identification and characterization of cleft proteins.
Burch A, Tao-Cheng JH, Dosemeci A
PloS one (2017) 123: e0174895. 243 003 WB, EM; tested species: rat
Cat. No.: 243 003
Amount: 50 µg
Price: $375.00
Altered expression of synaptic proteins and adhesion molecules in the hippocampus and cortex following the onset of diabetes in nonobese diabetic mice.
Yokokawa T, Kido K, Sato K, Hayashi T, Fujita S
Physiological reports (2023) 118: e15673. 243 003 WB; tested species: mouse
Haploinsufficiency of the Attention-Deficit/Hyperactivity Disorder Risk Gene St3gal3 in Mice Causes Alterations in Cognition and Expression of Genes Involved in Myelination and Sialylation.
Rivero O, Alhama-Riba J, Ku HP, Fischer M, Ortega G, Álmos P, Diouf D, van den Hove D, Lesch KP
Frontiers in genetics (2021) 12: 688488. 243 003 WB; tested species: mouse
A novel synaptic junction preparation for the identification and characterization of cleft proteins.
Burch A, Tao-Cheng JH, Dosemeci A
PloS one (2017) 123: e0174895. 243 003 WB, EM; tested species: rat
Molecular profiling of synaptic vesicle docking sites reveals novel proteins but few differences between glutamatergic and GABAergic synapses.
Boyken J, Grønborg M, Riedel D, Urlaub H, Jahn R, Chua JJ
Neuron (2013) 782: 285-97. 243 003 WB
A High-Resolution Method for Quantitative Molecular Analysis of Functionally Characterized Individual Synapses.
Holderith N, Heredi J, Kis V, Nusser Z
Cell reports (2020) 324: 107968. 243 003 IHC; tested species: rat
A novel synaptic junction preparation for the identification and characterization of cleft proteins.
Burch A, Tao-Cheng JH, Dosemeci A
PloS one (2017) 123: e0174895. 243 003 WB, EM; tested species: rat
WB
ICC
IHC
Background

Development of synapses involves transsynaptic interactions of dedicated synaptic adhesion molecules like neuroligins, neurexins and synCAMs (Synaptic cell adhesion molecules). Four SynCAM isoforms have been described so far. All share a common domain structure and contain three extracellular Ig domains, a single transmembrane region, and a short COOH-terminal cytoplasmic tail.
SynCAM 2 has been shown to be associated to myelinated axons and synCAM 3 can serve as a receptor for herpes viruses.
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