CLIP170 antibody - 239 003

Clip170 is a microtubule binding protein

Rabbit polyclonal purified antibody

Cat. No.: 239 003

Amount: 50 µg

Price: $375.00

Cat. No. 239 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: 1 : 200 up to 1 : 500 (see remarks) gallery IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 1283 to 1438 from human CLIP170 (UniProt Id: P30622)
Reactivity	Reacts with: human (P30622), rat (Q9JK25), mouse (Q922J3). Other species not tested yet.
Specificity	Recognizes CLIP 170 and restin.
Remarks	ICC: Fix 15 min at -20°C with ice-cold methanol / 1mM EGTA, then transfer without washing to 4% PFA (in PBS) and fix for 20 min at RT.
Data sheet	239_003.pdf

Cat. No.: 239 003

Amount: 50 µg

Price: $375.00

ICC

Background

Cytoplasmic linker proteins (CLIPs) are members of the plus-end-binding protein family (+TIPs) that interact with the growing plus ends of microtubules. They modulate microtubule dynamics and are proposed to link this cytoskeletal network to other intracellular structures.
CLIP 170 has been shown to colocalize with dynactin and dynein at microtubule ends and also at the kinetochore. High levels of a splice variant named restin are found in Hodgkin and Reed-Sternberg (HRS) cells, monocyte-derived dendritic cells (DCs) and interleukin-4 (IL-4) CD40L-activated B cells. Restin carries a 35 amino acids long insertion in the center of the molecule.

1 ) Gupta KK et al., J. Mol. Biol. (2010)
2 ) Tanenbaum ME et al., EMBO J. (2006)
3 ) Sahin U et al., Blood (2002)
4 ) Diamantopoulos GS et al., J. Cell Biol. (1999)
5 ) Griparic L et al., Biochim. Biophys. Acta (1998)
6 ) Bilbe G et al., EMBO J. (1992)
7 ) Pierre P et al., Cell (1992)

Protocols

ICC

Other products for this target