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CLIP 170 antibody - 239 003

Clip170 is a microtubule binding protein
Polyclonal rabbit purified antibody
Cat. No.: 239 003
Amount: 50 µg
Price: $370.00
Cat. No. 239 003 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Applications WB: 1 : 1000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 200 up to 1 : 500 (see remarks) gallery  
IHC: not tested yet
IHC-P/FFPE: not tested yet
Immunogen Recombinant protein corresponding to AA 1283 to 1438 from human CLIP170 (UniProt Id: P30622)
Reactivity Reacts with: human (P30622), rat (Q9JK25), mouse (Q922J3).
Other species not tested yet.
Specificity Recognizes CLIP 170 and restin.
Remarks

ICC: Fix 15 min at -20°C with ice-cold methanol / 1mM EGTA, then transfer without washing to 4% PFA (in PBS) and fix for 20 min at RT.

Data sheet 239_003.pdf
Cat. No.: 239 003
Quantity: 50 µg
Price: $370.00
Background
Cytoplasmic linker proteins (CLIPs) are members of the plus-end-binding protein family (+TIPs) that interact with the growing plus ends of microtubules. They modulate microtubule dynamics and are proposed to link this cytoskeletal network to other intracellular structures.
CLIP 170 has been shown to colocalize with dynactin and dynein at microtubule ends and also at the kinetochore. High levels of a splice variant named restin are found in Hodgkin and Reed-Sternberg (HRS) cells, monocyte-derived dendritic cells (DCs) and interleukin-4 (IL-4) CD40L-activated B cells. Restin carries a 35 amino acids long insertion in the center of the molecule.