Synaptic Systems - GABA-A receptor beta2

Cat. No.: 224 805

Amount: 50 µg

Price: $465.00

Cat. No. 224 805	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) (see remarks) gallery IP: yes ICC: 1 : 500 gallery IHC: 1 : 500 gallery IHC-P: not tested yet IHC-G: 1 : 500 (see remarks) gallery EM: yes
Immunogen	Recombinant protein corresponding to AA 343 to 430 from rat GABA-A receptor β2 (UniProt Id: P63138)
Reactivity	Reacts with: rat (P63138), mouse (P63137). Other species not tested yet.
Remarks	WB: GABA-A receptor beta2 aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples. IHC-G: Fixation with 3% glyoxal, 1% acetic acid, 20% ethanol in ddH2O according to Richter et al. 2017, or 9% glyoxal, 8% acetic acid in ddH2O according to Konno et al. 2023 are recommended.
Data sheet	224_805.pdf

The TMEM132B-GABAA receptor complex controls alcohol actions in the brain.
Wang G, Peng S, Reyes Mendez M, Keramidas A, Castellano D, Wu K, Han W, Tian Q, Dong L, Li Y, Lu W, et al.
Cell (2024) 18723: 6649-6668.e35. 224 805 ICC; tested species: mouse

Encephalitis patient-derived monoclonal GABAA receptor antibodies cause epileptic seizures.
Kreye J, Wright SK, van Casteren A, Stöffler L, Machule ML, Reincke SM, Nikolaus M, van Hoof S, Sanchez-Sendin E, Homeyer MA, Cordero Gómez C, et al.
The Journal of experimental medicine (2021) 21811: . 224 805 ICC, IHC; tested species: mouse,rat

Cat. No.: 224 805

Amount: 50 µg

Price: $465.00

ICC

IHC

IHC-G

Background

Protocols

GABA-A receptor beta2 antibody - 224 805