Tailor-made Antibodies
and Tools for Life Science

GABA-A receptor beta2 antibody - 224 803

GABA-A receptors are involved in inhibitory neurotransmission
Rabbit polyclonal purified antibody
Cat. No.: 224 803
Amount: 50 µg
Price: $380.00
Cat. No. 224 803 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) (see remarks) gallery  
IP: not tested yet
ICC: 1 : 500 gallery  
IHC: 1 : 500 (see remarks) gallery  
IHC-P: not tested yet
IHC-Fr: 1 : 500 (see remarks) gallery  
IHC-G: 1 : 500 (see remarks) gallery  
Immunogen Recombinant protein corresponding to AA 343 to 430 from rat GABA-A receptor β2 (UniProt Id: P63138)
Reactivity Reacts with: rat (P63138), mouse (P63137).
Other species not tested yet.
Remarks

WB:  GABA-A receptor beta2 aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.
IHC: This antibody requires antigen retrieval with pepsin according to:
Lorincz A & Nusser Z (2008).
IHC-Fr: Fixation with acetone or PFA/formaldehyde is recommended.
IHC-G: Fixation with 3% glyoxal, 1% acetic acid, 20% ethanol in ddH2O according to Richter et al. 2017, or 9% glyoxal, 8% acetic acid in ddH2O according to Konno et al. 2023 are recommended.

Data sheet 224_803.pdf

References for GABA-A receptor beta2 - 224 803

The TMEM132B-GABAA receptor complex controls alcohol actions in the brain.
Wang G, Peng S, Reyes Mendez M, Keramidas A, Castellano D, Wu K, Han W, Tian Q, Dong L, Li Y, Lu W, et al.
Cell (2024) 18723: 6649-6668.e35. 224 803 WB, ICC; tested species: mouse
Activating Transcription Factor 4 (ATF4) regulates neuronal activity by controlling GABABR trafficking.
Corona C, Pasini S, Liu J, Amar F, Greene LA, Shelanski ML
The Journal of neuroscience : the official journal of the Society for Neuroscience (2018) : . 224 803 WB; tested species: rat
Cat. No.: 224 803
Amount: 50 µg
Price: $380.00