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- will be discontinued soon

Polyclonal antibodies are a finite resource. We have now started to sell the last lots of our well received rabbit polyclonal GABA-A receptor alpha5 antibody 224 502.

GABA-A receptor alpha5 antibody extracellular - 224 502

GABA-A receptors are involved in inhibitory neurotransmission

Rabbit polyclonal antiserum

Cat. No.: 224 502

Amount: 200 µl

Price: $355.00

Cat. No. 224 502	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) (see remarks) gallery IP: not tested yet ICC: not tested yet IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 26 to 43 from rat GABA-A receptor α5 (UniProt Id: P19969)
Reactivity	Reacts with: rat (P19969), mouse (Q8BHJ7). Other species not tested yet.
Matching control protein/peptide	224-5P
Remarks	WB: GABA-A receptor alpha5 aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.
Data sheet	224_502.pdf

Cat. No.: 224 502

Amount: 200 µl

Price: $355.00

Background

Gamma-aminobutyric acid type A (GABA-A) receptors mediate the majority of inhibitory neurotransmission in the brain. These receptor proteins are ligand gated chloride ion channels and consist of a pentameric combination of different subunits (alpha, beta, gamma, delta, epsilon and rho). The resulting heterogenous population of GABA-A receptor subtypes are expressed throughout the brain with specific cellular and subcellular expression patterns.

1 ) Laurie DJ et al., J. Neurosci. (1992)
2 ) Laurie DJ et al., J. Neurosci. (1992)
3 ) Wisden W et al., J. Neurosci. (1992)
4 ) Lüddens H et al., Nature (1990)
5 ) Sassoè-Pognetto M et al., Ann. N. Y. Acad. Sci. (2009)
6 ) Viltono L et al., J. Comp. Neurol. (2008)
7 ) Kralic JE et al., J. Comp. Neurol. (2006)
8 ) Essrich C et al., Nat. Neurosci. (1998)
9 ) Fritschy JM et al., J. Comp. Neurol. (1995)
10 ) Benke D et al., J. Biol. Chem. (1994)

Protocols

OTHER

Preabsorbtion

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