Synaptic Systems - GABA-A receptor gamma2

Cat. No.: 224 011

Amount: 100 µg

Price: $415.00

Cat. No. 224 011	100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not tested yet IP: yes ICC: 1 : 500 (see remarks) gallery IHC: 1 : 500 (see remarks) gallery IHC-P: not tested yet EM: yes Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Electron microscopy (EM) is a microscopy technique that detects the scatter of electrons through thin tissue sections. In Immuno-EM the antigen is usually revealed by colloidal gold conjugated secondary antibodies linking electron dense structures to antigen-bound primary antibodies.
Clone	331A12
Subtype	IgG2a (κ light chain)
Immunogen	Synthetic peptide corresponding to AA 39 to 67 from mouse GABA-A receptor γ2 (UniProt Id: P22723)
Reactivity	Reacts with: human (P18507), rat (P18508), mouse (P22723). Other species not tested yet.
Specificity	Specific for GABA-A receptor γ2. Does not discriminate between the L and S form.
Matching control protein/peptide	224-0P
Remarks	ICC: This antibody is also suitable for the surface staining of living cells. After washing cells with bound antibodies, they can be fixed and visualized with secondary reagents. IHC: This antibody requires mild fixation.
Data sheet	224_011.pdf

References for GABA-A receptor gamma2 - 224 011

Structural Basis for Teneurin Function in Circuit-Wiring: A Toxin Motif at the Synapse.
Li J, Shalev-Benami M, Sando R, Jiang X, Kibrom A, Wang J, Leon K, Katanski C, Nazarko O, Lu YC, Südhof TC, et al.
Cell (2018) 1733: 735-748.e15. 224 011 ICC; tested species: mouse

A High-Resolution Method for Quantitative Molecular Analysis of Functionally Characterized Individual Synapses.
Holderith N, Heredi J, Kis V, Nusser Z
Cell reports (2020) 324: 107968. 224 011 IHC; tested species: rat

Interneurons and oligodendrocyte progenitors form a structured synaptic network in the developing neocortex.
Orduz D, Maldonado PP, Balia M, Vélez-Fort M, de Sars V, Yanagawa Y, Emiliani V, Angulo MC
eLife (2015) 4: . 224 011 IHC; tested species: mouse

Similar GABAA receptor subunit composition in somatic and axon initial segment synapses of hippocampal pyramidal cells.
Kerti-Szigeti K, Nusser Z
eLife (2016) 5: . 224 011 EM

Cat. No.: 224 011

Amount: 100 µg

Price: $415.00

Similar GABAA receptor subunit composition in somatic and axon initial segment synapses of hippocampal pyramidal cells.
Kerti-Szigeti K, Nusser Z
eLife (2016) 5: . 224 011 EM

ICC

IHC

Background

Gamma-aminobutyric acid type A (GABA-A) receptors mediate the majority of inhibitory neurotransmission in the brain. These receptor proteins are ligand gated chloride ion channels and consist of a pentameric combination of different subunits (alpha, beta, gamma, delta, epsilon and rho). The resulting heterogenous population of GABA-A receptor subtypes are expressed throughout the brain with specific cellular and subcellular expression patterns.

1 ) Sassoè-Pognetto M et al., Ann. N. Y. Acad. Sci. (2009)
2 ) Viltono L et al., J. Comp. Neurol. (2008)
3 ) Kralic JE et al., J. Comp. Neurol. (2006)
4 ) Essrich C et al., Nat. Neurosci. (1998)
5 ) Fritschy JM et al., J. Comp. Neurol. (1995)
6 ) Benke D et al., J. Biol. Chem. (1994)
7 ) Fritschy JM et al., Proc. Natl. Acad. Sci. U.S.A. (1992)

Protocols

Standard protocol

ICC

IHC

OTHER

Preabsorbtion

Other products for this target

GABA-A receptor gamma2 antibody extracellular - 224 011

References for GABA-A receptor gamma2 - 224 011