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m-AChR-2 - 223 017

A G protein-coupled acetylcholine receptor
Monoclonal rat purified IgG
Cat. No.: 223 017
Amount: 100 µg
Price: 415.00 €
Cat. No. 223 017 100 µg purified IgG, lyophilized. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.
Applications WB: 1 : 1000 (AP staining) gallery  
IP: not tested yet
ICC: not tested yet
IHC: 1 : 100 gallery  
IHC-P/FFPE: not tested yet
Clone 2-1-155
Subtype IgG1
Immunogen 207 to 388 from human m-AChR-2 (UniProt Id: P08172)
Epitop Epitop: AA 207 to 388 from human m-AChR-2 (UniProt Id: P08172)
Reactivity Reacts with: human (P08172), rat (P10980), mouse (Q9ERZ4).
Other species not tested yet.
Specificity Specific for muscarinic acetylcholine receptor 2.
Remarks This antibody detects also higher molecular weight bands, depending on the glycosilation state of the protein.The protein tends to aggregate after boiling, making it necessary to run SDS-PAGE with non-boiled samples.
Data sheet 223_017.pdf

References for m-AChR-2 - 223 017

Evaluation of muscarinic agonist-induced analgesia in muscarinic acetylcholine receptor knockout mice.
Duttaroy A, Gomeza J, Gan JW, Siddiqui N, Basile AS, Harman WD, Smith PL, Felder CC, Levey AI, Wess J
Molecular pharmacology (2002) 625: 1084-93. 223 017 IHC
Subcellular redistribution of m2 muscarinic acetylcholine receptors in striatal interneurons in vivo after acute cholinergic stimulation.
Bernard V, Laribi O, Levey AI, Bloch B
The Journal of neuroscience : the official journal of the Society for Neuroscience (1998) 1823: 10207-18. 223 017 IHC
Light and electron microscopic study of m2 muscarinic acetylcholine receptor in the basal forebrain of the rat.
Levey AI, Edmunds SM, Hersch SM, Wiley RG, Heilman CJ
The Journal of comparative neurology (1995) 3513: 339-56. 223 017 IHC
Cat. No.: 223 017
Quantity: 100 µg
Price: 415.00 €
Evaluation of muscarinic agonist-induced analgesia in muscarinic acetylcholine receptor knockout mice.
Duttaroy A, Gomeza J, Gan JW, Siddiqui N, Basile AS, Harman WD, Smith PL, Felder CC, Levey AI, Wess J
Molecular pharmacology (2002) 625: 1084-93. 223 017 IHC
Subcellular redistribution of m2 muscarinic acetylcholine receptors in striatal interneurons in vivo after acute cholinergic stimulation.
Bernard V, Laribi O, Levey AI, Bloch B
The Journal of neuroscience : the official journal of the Society for Neuroscience (1998) 1823: 10207-18. 223 017 IHC
Light and electron microscopic study of m2 muscarinic acetylcholine receptor in the basal forebrain of the rat.
Levey AI, Edmunds SM, Hersch SM, Wiley RG, Heilman CJ
The Journal of comparative neurology (1995) 3513: 339-56. 223 017 IHC
Background
The muscarinic acetylcholine receptors comprise 5 members (m1 - m5) and mediate many acetylcholine driven cellular actions such as adenylate cyclase inhibition, phosphoinositide degeneration and potassium channel mediation. They belong to a larger family of G protein-coupled receptors. Muscarinic acetylcholine receptor 2 has been suggested to function as a presynaptic autoreceptor that inhibits acetylcholine release in the basal forebrain. It is also expressed in cardiac tissue where it is involved in mediation of bradycardia and a decrease in cardiac contractility.