Tailor-made Antibodies
and Tools for Life Science

m-AChR-2 antibody - 223 017

m-AChR-2 is a G protein-coupled acetylcholine receptor
Rat monoclonal purified IgG
Cat. No.: 223 017
Amount: 100 µg
Price: $420.00
Cat. No. 223 017 100 µg purified IgG, lyophilized. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) (see remarks) gallery  
IP: not tested yet
ICC: not tested yet
IHC: 1 : 100 gallery  
IHC-P: not tested yet
Clone 2-1-155
Subtype IgG1
Immunogen 207 to 388 from human m-AChR-2 (UniProt Id: P08172)
Epitop AA 207 to 388 from human m-AChR-2 (UniProt Id: P08172)
Reactivity Reacts with: human (P08172), rat (P10980), mouse (Q9ERZ4).
Other species not tested yet.
Remarks

WB: This antibody detects also higher molecular weight bands, depending on the glycosilation state of the protein.The protein tends to aggregate after boiling, making it necessary to run SDS-PAGE with non-boiled samples.

Data sheet 223_017.pdf

References for m-AChR-2 - 223 017

INSIHGT: an accessible multi-scale, multi-modal 3D spatial biology platform.
Yau CN, Hung JTS, Campbell RAA, Wong TCY, Huang B, Wong BTY, Chow NKN, Zhang L, Tsoi EPL, Tan Y, Li JJX, et al.
Nature communications (2024) 151: 10888. 223 017 IHC; tested species: mouse
Evaluation of muscarinic agonist-induced analgesia in muscarinic acetylcholine receptor knockout mice.
Duttaroy A, Gomeza J, Gan JW, Siddiqui N, Basile AS, Harman WD, Smith PL, Felder CC, Levey AI, Wess J
Molecular pharmacology (2002) 625: 1084-93. 223 017 IHC
Subcellular redistribution of m2 muscarinic acetylcholine receptors in striatal interneurons in vivo after acute cholinergic stimulation.
Bernard V, Laribi O, Levey AI, Bloch B
The Journal of neuroscience : the official journal of the Society for Neuroscience (1998) 1823: 10207-18. 223 017 IHC
Light and electron microscopic study of m2 muscarinic acetylcholine receptor in the basal forebrain of the rat.
Levey AI, Edmunds SM, Hersch SM, Wiley RG, Heilman CJ
The Journal of comparative neurology (1995) 3513: 339-56. 223 017 IHC
Cat. No.: 223 017
Amount: 100 µg
Price: $420.00