|Cat. No. 218 703||50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.|
WB: 1 : 1000 (ECL detection) (see remarks) gallery
IP: not tested yet
ICC: not tested yet
IHC: 1 : 100 up to 1 : 500 (see remarks) gallery
IHC-P/FFPE: 1 : 100 (see remarks) gallery
ELISA: yes suitable only as capture antibody, cat. no. 218 211 is recommended detector antibody for gallery (protocol)
|Immunogen||Synthetic peptide corresponding to AA 37 to 42 from human Abeta42 (UniProt Id: P05067)|
Reacts with: human (P05067), rat (P08592), mouse (P12023).
Other species not tested yet.
|Specificity||Specific for Abeta 42 with weak cross-reactivity to Abeta 40 in westernblots that is not apparent in ELISA tests.|
WB: Detects purified Abeta 42. Complex samples like brain extracts still have to be tested. Nitrocellulose membrane is recommended for blotting. Boil membrane after blotting for 3min.
Amyloid deposits, also called plaques, of Alzheimer's patients consist of several protein components like the amyloid beta-peptides (Abeta, Aβ) 1-40/42/43 and additional C- and N-terminally modified fragments of Abeta as for instance Abeta pE3 and Abeta pE11.
An additional Abeta variant, Abeta 38, is more soluble compared to other Abeta species and is not found in plaques of sporadic Alzheimer´s cases. However, it is detected in the blood-vessel walls of a subset of patients with severe cerebral amyloid angiopathy. It especially accumulates in brains of patients carrying mutations in the Abeta coding region.
Cleavage of amyloid precursor protein APP by β- and γ- secretases results in the generation of the Aβ (βA4)peptide, whereas α-secretase cleaves within the Aβ sequence and prevents the formation of Abeta from APP.