Tailor-made Antibodies
and Tools for Life Science

Abeta42 antibody - 218 703

Abeta peptides are major components of neuritic plaques in Alzheimer's disease
Rabbit polyclonal purified antibody
Cat. No.: 218 703
Amount: 50 µg
Price: $385.00
Cat. No. 218 703 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (ECL detection) (see remarks) gallery  
IP: not tested yet
ICC: not tested yet
IHC: 1 : 100 up to 1 : 500 (see remarks) gallery  
IHC-P: 1 : 100 (see remarks) gallery  
ELISA: yes suitable only as capture antibody, cat. no. 218 211 is recommended detector antibody for (see remarks) gallery  
Immunogen Synthetic peptide corresponding to AA 37 to 42 from human Abeta42 (UniProt Id: P05067)
Reactivity Reacts with: human (P05067), rat (P08592), mouse (P12023).
Other species not tested yet.
Specificity Specific for Abeta 42 with weak cross-reactivity to Abeta 40 in westernblots that is not apparent in ELISA tests.
Remarks

WB: Detects purified Abeta 42. Complex samples like brain extracts still have to be tested. Nitrocellulose membrane is recommended for blotting. Boil membrane after blotting for 3min.
IHC: Antigen retrieval with formic acid is required.
IHC-P: Antigen retrieval with formic acid is required.
ELISA: suitable as capture antibody with cat. no. 218 211 as detector antibody.

Data sheet 218_703.pdf

References for Abeta42 - 218 703

Astrocytes infected with Chlamydia pneumoniae demonstrate altered expression and activity of secretases involved in the generation of β-amyloid found in Alzheimer disease.
Al-Atrache Z, Lopez DB, Hingley ST, Appelt DM
BMC neuroscience (2019) 201: 6. 218 703 ICC; tested species: human
Cat. No.: 218 703
Amount: 50 µg
Price: $385.00