Synaptic Systems - Abeta38/40/42/43

Cat. No.: 218 111BT

Amount: 100 µg

Price: $465.00

IHC

IHC-P

Background

Amyloid deposits, also called plaques, of Alzheimer's patients consist of several protein components like the amyloid beta-peptides (Abeta, Aβ) 1-40/42/43 and additional C- and N-terminally modified fragments of Abeta as for instance Abeta pE3 and Abeta pE11.
An additional Abeta variant, Abeta38, is more soluble compared to other Abeta species and is not found in plaques of sporadic Alzheimer´s cases. However, it is detected in the blood-vessel walls of a subset of patients with severe cerebral amyloid angiopathy. It especially accumulates in brains of patients carrying mutations in the Abeta coding region.
Cleavage of amyloid precursor protein APP by β- and γ- secretases results in the generation of the Aβ (βA4)peptide, whereas α-secretase cleaves within the Aβ sequence and prevents the formation of Abeta from APP.

1 ) Marcello A et al., J Neural Transm (Vienna) (2009)
2 ) Baril L et al., Neurosci. Lett. (2004)
3 ) Bayer TA et al., Proc. Natl. Acad. Sci. U.S.A. (2003)
4 ) Hsiao K et al., Science (1996)
5 ) Wood SJ et al., J. Mol. Biol. (1996)
6 ) Kuo YM et al., J. Biol. Chem. (1996)

Protocols

WB

IHC

IHC-P

Other products for this target

Cat. No. 218 111BT	100 µg purified IgG, lyophilized, labeled with Biotin. Biotin is a small vitamin B complex molecule. Its small size minimizes interference with antibody binding sites and allows for efficient conjugation without compromising antibody functionality. Biotin conjugated antibodies can be easily detected with Avidin/Streptavidin based secondary detection systems. The streptavidin-biotin bond is one of the strongest non-covalent biological bonds known. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 500 up to 1 : 1000 (see remarks) IP: not tested yet ICC: not tested yet IHC: 1 : 200 up to 1 : 500 (see remarks) gallery IHC-P: 1 : 200 up to 1 : 500 (see remarks) gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Label	biotin
Clone	NT78
Subtype	IgG2b (κ light chain)
Immunogen	Synthetic peptide corresponding to AA 1 to 16 from human Abeta (UniProt Id: P05067)
Epitop	AA 4 to 16 from human Abeta (UniProt Id: P05067)
Reactivity	Reacts with: human (P05067), mouse (P12023). Other species not tested yet.
Specificity	Specific for Abeta 38, 40, 42, 43. Due to sequence homology, a cross-reactivity to unprocessed APP cannot be excluded.
Remarks	WB: Boil membrane after blotting for 3min. IHC: Antigen retrieval with formic acid is required. IHC-P: Antigen retrieval with formic acid is required.
Data sheet	218_111bt.pdf

Abeta38/40/42/43 antibody - 218 111BT