Synaptic Systems - Tenascin-C

Cat. No.: 217 127

Amount: 200 µl

Price: $365.00

Cat. No. 217 127	200 µl hybridoma supernatant, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 500 up to 1 : 1000 (AP staining) gallery IP: not tested yet ICC: 1 : 100 up to 1 : 500 gallery IHC: 1 : 500 gallery IHC-P (FFPE): 1 : 500 up to 1 : 1000 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	578
Subtype	IgG2a
Immunogen	Recombinant protein corresponding to AA 23 to 2210 from mouse Tenascin-C (UniProt Id: Q80YX1)
Epitop	AA 1082 to 1510 from mouse Tenascin-C (UniProt Id: Q80YX1)
Reactivity	Reacts with: rat, mouse (Q80YX1). Other species not tested yet.
Specificity	Specific for tenascin-C splice variants carrying the FNIII D domain.
Remarks	Tenascin-C variants detected by this antibody are downregulated during development and hardly detectable in adult animals
Data sheet	Datasheet 217_127

References for Tenascin-C - 217 127

Tenascin-C promotes neurite outgrowth of embryonic hippocampal neurons through the alternatively spliced fibronectin type III BD domains via activation of the cell adhesion molecule F3/contactin.
Rigato F, Garwood J, Calco V, Heck N, Faivre-Sarrailh C, Faissner A
The Journal of neuroscience : the official journal of the Society for Neuroscience (2002) 2215: 6596-609. 217 127 WB

Expression of tenascin in the developing and adult cerebellar cortex.
Bartsch S, Bartsch U, Dörries U, Faissner A, Weller A, Ekblom P, Schachner M
The Journal of neuroscience : the official journal of the Society for Neuroscience (1992) 123: 736-49. 217 127 WB, IHC

The glia-derived extracellular matrix glycoprotein tenascin-C promotes embryonic and postnatal retina axon outgrowth via the alternatively spliced fibronectin type III domain TNfnD.
Siddiqui S, Horvat-Bröcker A, Faissner A
Neuron glia biology (2008) 44: 271-83. 217 127 ICC

Fibroblasts that proliferate near denervated synaptic sites in skeletal muscle synthesize the adhesive molecules tenascin(J1), N-CAM, fibronectin, and a heparan sulfate proteoglycan.
Gatchalian CL, Schachner M, Sanes JR
The Journal of cell biology (1989) 1085: 1873-90. 217 127 ICC, IHC

J1/tenascin-related molecules are not responsible for the segmented pattern of neural crest cells or motor axons in the chick embryo.
Stern CD, Norris WE, Bronner-Fraser M, Carlson GJ, Faissner A, Keynes RJ, Schachner M
Development (Cambridge, England) (1989) 1072: 309-19. 217 127 IHC

Cat. No.: 217 127

Amount: 200 µl

Price: $365.00

ICC

IHC

IHC-P (FFPE)

Background

Tenascin-C, also referrred to as TN-C, Cytoactin, and J1-200/220, is a multimodular glycoprotein with neurite outgrowth-stimulating properties. It is composed of a cysteine rich amino-terminus followed by a stretch of EGF like and fibronectin type III (FNIII) repeats. Its C-terminus shows homologies to fibrinogen β and γ. In the central nervous system TN-C is transiently expressed by immature astrocytes and by subpopulations of neurons, e.g., retinal ganglion cells.

1 ) Flück M et al., Proc. Natl. Acad. Sci. U.S.A. (2008)
2 ) Irintchev A et al., Cereb. Cortex (2005)
3 ) Rigato F et al., J. Neurosci. (2002)
4 ) Götz B et al., J. Cell Biol. (1996)
5 ) Gatchalian CL et al., J. Cell Biol. (1989)

Protocols

ICC

Fixed Cells

IHC

IHC-P (FFPE)

Tenascin-C antibody - 217 127

References for Tenascin-C - 217 127