Tyrosine hydroxylase antibody - 213 108

Tyrosine hydroxylase is the rate limiting enzyme in catecholamine synthesis

Rabbit monoclonal recombinant IgG

Cat. No.: 213 108

Amount: 50 µg

Price: $415.00

Cat. No. 213 108	50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP-staining) gallery IP: not tested yet ICC: 1 : 500 up to 1 : 1000 gallery IHC: 1 : 500 up to 1 : 1000 gallery IHC-P: 1 : 1000 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	Rb43F2
Subtype	IgG1 (κ light chain)
Immunogen	Recombinant protein corresponding to residues near the amino-terminus of rat TyrH. (UniProt Id: P04177)
Reactivity	Reacts with: mouse (P24529), rat (P04177). Other species not tested yet.
Data sheet	213_108.pdf

Cat. No.: 213 108

Amount: 50 µg

Price: $415.00

ICC

IHC

IHC-P

Background

Tyrosine hydroxylase is one of the key enzymes in the synthesis pathway of catecholamines like adrenalin, noradrenalin and dopamin and is frequently used as a marker for dopaminergic neurons. This neuronal subpopulation is especially affected in Parkinson's disease.

1 ) Vadasz C et al., Neuroscience (2007)
2 ) Rao F et al., Circulation (2007)
3 ) Lehmann IT et al., J. Biol. Chem. (2006)
4 ) Peng X et al., J. Cell. Sci. (2005)
5 ) Jeon YK et al., Mol. Cells (2001)
6 ) McLean JH et al., J. Neurosci. (1988)
7 ) Musacchio JM et al., Proc. Natl. Acad. Sci. U.S.A. (1969)

Protocols

ICC

IHC

IHC-P

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