Synaptic Systems - Tyrosine hydroxylase

Cat. No.: 213 104

Amount: 100 µl

Price: $370.00

Cat. No. 213 104	100 µl antiserum, lyophilized. For reconstitution add 100 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes ICC: not tested yet IHC: 1 : 500 gallery IHC-P: 1 : 500 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to residues near the amino-terminus of rat TyrH. (UniProt Id: P04177)
Reactivity	Reacts with: rat (P04177), mouse (P24529). Other species not tested yet.
Data sheet	213_104.pdf

References for Tyrosine hydroxylase - 213 104

The proteomic landscape of synaptic diversity across brain regions and cell types.
van Oostrum M, Blok TM, Giandomenico SL, Tom Dieck S, Tushev G, Fürst N, Langer JD, Schuman EM
Cell (2023) 18624: 5411-5427.e23. 213 104 IHC, WB; tested species: mouse

Functional hyperactivity in long QT syndrome type 1 pluripotent stem cell-derived sympathetic neurons.
Winbo A, Ramanan S, Eugster E, Rydberg A, Jovinge S, Skinner JR, Montgomery JM
American journal of physiology. Heart and circulatory physiology (2021) 3211: H217-H227. 213 104 ICC; tested species: human

Synaptotagmin-1 is the Ca2+ sensor for fast striatal dopamine release.
Banerjee A, Lee J, Nemcova P, Liu C, Kaeser PS
eLife (2020) 9: . 213 104 ICC, IHC; tested species: mouse

Functional Coculture of Sympathetic Neurons and Cardiomyocytes Derived from Human Induced Pluripotent Stem Cells.
Winbo A, Ramanan S, Eugster E, Jovinge S, Skinner JR, Montgomery JM
American journal of physiology. Heart and circulatory physiology (2020) : . 213 104 ICC; tested species: human

Dopamine Secretion Is Mediated by Sparse Active Zone-like Release Sites.
Liu C, Kershberg L, Wang J, Schneeberger S, Kaeser PS
Cell (2018) 1724: 706-718.e15. 213 104 ICC, IHC; tested species: mouse

Coordinating brain-distributed network activities in memory resistant to extinction.
Clarke-Williams CJ, Lopes-Dos-Santos V, Lefèvre L, Brizee D, Causse AA, Rothaermel R, Hartwich K, Perestenko PV, Toth R, McNamara CG, Sharott A, et al.
Cell (2024) 1872: 409-427.e19. 213 104 IHC; tested species: mouse

Deficiency of Perry syndrome-associated p150Glued in midbrain dopaminergic neurons leads to progressive neurodegeneration and endoplasmic reticulum abnormalities.
Yu J, Yang X, Zheng J, Sgobio C, Sun L, Cai H
NPJ Parkinson's disease (2023) 91: 35. 213 104 IHC; tested species: mouse

Neuronal Adenosine A1 Receptor is Critical for Olfactory Function but Unable to Attenuate Olfactory Dysfunction in Neuroinflammation.
Schubert C, Schulz K, Träger S, Plath AL, Omriouate A, Rosenkranz SC, Morellini F, Friese MA, Hirnet D
Frontiers in cellular neuroscience (2022) 16: 912030. 213 104 IHC; tested species: mouse

A glutaminyl cyclase-catalyzed α-synuclein modification identified in human synucleinopathies.
Hartlage-Rübsamen M, Bluhm A, Moceri S, Machner L, Köppen J, Schenk M, Hilbrich I, Holzer M, Weidenfeller M, Richter F, Coras R, et al.
Acta neuropathologica (2021) : . 213 104 IHC; tested species: mouse

Presence of ethanol-sensitive and ethanol-insensitive glycine receptors in the ventral tegmental area and prefrontal cortex in mice.
Araya A, Gallegos S, Viveros R, San Martin L, Muñoz B, Harvey RJ, Zeilhofer HU, Aguayo LG
British journal of pharmacology (2021) 17823: 4691-4707. 213 104 IHC; tested species: mouse

Evidence of structural and functional plasticity occurring within the intracardiac nervous system of spontaneously hypertensive rats.
Ashton JL, Argent LP, Smith JE, Jin S, Sands GB, Smaill BH, Montgomery JM
American journal of physiology. Heart and circulatory physiology (2020) : . 213 104 IHC; tested species: rat

Synaptotagmin-1 is the Ca2+ sensor for fast striatal dopamine release.
Banerjee A, Lee J, Nemcova P, Liu C, Kaeser PS
eLife (2020) 9: . 213 104 ICC, IHC; tested species: mouse

THC Regulates Tearing via Cannabinoid CB1 Receptors.
Thayer A, Murataeva N, Delcroix V, Wager-Miller J, Makarenkova HP, Straiker A
Investigative ophthalmology & visual science (2020) 6110: 48. 213 104 IHC; tested species: mouse

In vivo functional diversity of midbrain dopamine neurons within identified axonal projections.
Farassat N, Costa KM, Stojanovic S, Albert S, Kovacheva L, Shin J, Egger R, Somayaji M, Duvarci S, Schneider G, Roeper J, et al.
eLife (2019) 8: . 213 104 IHC; tested species: mouse

Characterization of the axon initial segment of mice substantia nigra dopaminergic neurons.
González-Cabrera C, Meza R, Ulloa L, Merino-Sepúlveda P, Luco V, Sanhueza A, Oñate-Ponce A, Bolam JP, Henny P
The Journal of comparative neurology (2017) 52516: 3529-3542. 213 104 IHC; tested species: mouse

Spike-Dependent Dynamic Partitioning of the Locus Coeruleus Network through Noradrenergic Volume Release in a Simulation of the Nucleus Core.
Baral S, Hosseini H, More K, Fabrin TMC, Braun J, Prigge M
Brain sciences (2022) 126: . 213 104 ExM; tested species: mouse

Multiplex imaging of human induced pluripotent stem cell-derived neurons with CO-Detection by indEXing (CODEX) technology.
Heinrich L, Zafar F, Morato Torres CA, Singh J, Khan A, Chen MY, Hempel C, Nikulina N, Mulholland J, Braubach O, Schüle B, et al.
Journal of neuroscience methods (2022) : 109653. 213 104 Codex-PC; tested species: human

Cat. No.: 213 104

Amount: 100 µl

Price: $370.00

Synaptotagmin-1 is the Ca2+ sensor for fast striatal dopamine release.
Banerjee A, Lee J, Nemcova P, Liu C, Kaeser PS
eLife (2020) 9: . 213 104 ICC, IHC; tested species: mouse

IHC

IHC-P

Background

Tyrosine hydroxylase is one of the key enzymes in the synthesis pathway of catecholamines like adrenalin, noradrenalin and dopamin and is frequently used as a marker for dopaminergic neurons. This neuronal subpopulation is especially affected in Parkinson's disease.

1 ) Vadasz C et al., Neuroscience (2007)
2 ) Rao F et al., Circulation (2007)
3 ) Lehmann IT et al., J. Biol. Chem. (2006)
4 ) Peng X et al., J. Cell. Sci. (2005)
5 ) Jeon YK et al., Mol. Cells (2001)
6 ) McLean JH et al., J. Neurosci. (1988)
7 ) Musacchio JM et al., Proc. Natl. Acad. Sci. U.S.A. (1969)

Protocols

Standard protocol

IHC

IHC-P

Other products for this target

Tyrosine hydroxylase antibody - 213 104

References for Tyrosine hydroxylase - 213 104