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Home > Products > 202 018

m6A antibody - 202 018

N6-methyladenosine or m6A are posttranscritional modifications of RNA and DNA
Rabbit monoclonal recombinant IgG
Cat. No.: 202 018
Amount: 100 µg
Price: $415.00
Cat. No. 202 018 100 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
Dot blot: 1 : 1000 up to 1 : 2000 gallery  
IP: yes
MeRIP: yes
Clone Rb345E11
Subtype IgG1 (κ light chain)
Immunogen N6-methyladenosine fused to BSA.
Reactivity Reacts with: human, rat, mouse, prokaryotes, eukaryotes.
Other species not tested yet.
Specificity Specific for N6-methyladenosine (m6A) with some cross-reactivity to m6Am
Remarks

This antibody is a chimeric antibody based on the well known monoclonal mouse antibody clone 345E11. The constant regions of the heavy and light chains have been replaced by rabbit specific sequences. Therefore, the antibody can be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells.
Data sheet 202_018.pdf
Cat. No.: 202 018
Amount: 100 µg
Price: $415.00
Dot blot
Background
m6A (N6-methyladenosine) is a posttranscriptional RNA-modification found throughout all kingdoms, e.g. in vertebrate snRNAs U2, U4, U6, in viral and eukaryotic mRNAs, and in E. coli 16S rRNA.
Recent studies have found that mRNA is predominately m6A modified at stop codons and long internal exons, which are conserved between mouse and human. The so-called RNA methylome probably plays an important role in in the regulation of gene expression.
In E. coli Dam methylase introduces m6A modifications on the DNA level at the 5'-GATC-3' motif. This allows the cell to differentiate between the parental and the daughter strand during mismatch repair.
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