Tailor-made Antibodies
and Tools for Life Science

m6A antibody - 202 003

N6-methyladenosine or m6A are posttranscritional modifications of RNA and DNA
Rabbit polyclonal purified antibody
Cat. No.: 202 003
Amount: 50 µg
Price: $480.00
Cat. No. 202 003 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
Dot blot: 1 : 1000 up to 1 : 10000 gallery  
IP: yes
ICC: not tested yet
IHC: not tested yet
IHC-P: not tested yet
ELISA: yes suitable for sandwich-ELISA gallery  
MeRIP: yes
Immunogen N6-methyladenosine fused to BSA
Reactivity Reacts with: human, rat, mouse, eukaryotes, prokaryotes.
Other species not tested yet.
Specificity Specific for N6-methyladenosine (m6A) with some cross-reactivity to m6Am.
Data sheet 202_003.pdf

References for m6A - 202 003

Epstein-Barr virus suppresses N6-Methyladenosine modification of TLR9 to promote immune evasion.
Zhang X, Li Z, Peng Q, Liu C, Wu Y, Wen Y, Zheng R, Xu C, Tian J, Zheng X, Yan Q, et al.
The Journal of biological chemistry (2024) : 107226. 202 003 WB, MeRIP, Dot blot; tested species: human
METTL3-mediated m6A modification of circGLIS3 promotes prostate cancer progression and represents a potential target for ARSI therapy.
Cheng X, Yang H, Chen Y, Zeng Z, Liu Y, Zhou X, Zhang C, Xie A, Wang G
Cellular & molecular biology letters (2024) 291: 109. 202 003 WB, MeRIP; tested species: human
The methyltransferase METTL3 regulates endothelial cell proliferation and inflammation via m6A RNA methylation-mediated TRAF1 expression.
Chen D, Xu W, Zheng H, Zhang Y, Lin Y, Han Y, Yao F, Shen H
Biochemical and biophysical research communications (2024) 732: 150399. 202 003 WB, MeRIP; tested species: human
Immuno-Northern Blotting: Detection of Modified RNA Using Gel Separation and Antibodies to Modified Nucleosides.
Mishima E, Abe T
Methods in molecular biology (Clifton, N.J.) (2019) 1870: 179-187. 202 003 WB
HIV-1 envelope proteins up-regulate N6-methyladenosine levels of cellular RNA independently of viral replication.
Tirumuru N, Wu L
The Journal of biological chemistry (2019) : . 202 003 WB, Dot blot; tested species: human
Immuno-Northern Blotting: Detection of RNA Modifications by Using Antibodies against Modified Nucleosides.
Mishima E, Jinno D, Akiyama Y, Itoh K, Nankumo S, Shima H, Kikuchi K, Takeuchi Y, Elkordy A, Suzuki T, Niizuma K, et al.
PloS one (2015) 1011: e0143756. 202 003 WB, Dot blot
Cat. No.: 202 003
Amount: 50 µg
Price: $480.00