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m3G-cap, m7G-cap - 201 011

Antibodies against posttranscripitional modifications of RNAs
Monoclonal mouse purified IgG
Cat. No.: 201 011
Amount: 100 µg
Price: $415.00
Cat. No. 201 011 100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Applications WB: not recommended
IP: yes
ICC: yes (see reference)
IHC: not tested yet
IHC-P/FFPE: not tested yet
ELISA: yes
Clone H20
Subtype IgG1 (κ light chain)
Immunogen Synthetic m3G-cap conjugated to human serum albumin.
Reactivity Reacts with: human, rat, mouse, eukaryotes.
Other species not tested yet.
Specificity Recognizes m3G-cap and m7G-cap.
Remarks

This antibody can be used to detect capped RNAs (e.g. in viruses) or to identify and purify proteins associated with capped RNAs (see reference #2).

Data sheet 201_011.pdf

References for m3G-cap, m7G-cap - 201 011

Capping pores of alphavirus nsP1 gate membranous viral replication factories.
Jones R, Bragagnolo G, Arranz R, Reguera J
Nature (2020) : . 201 011 WB
Approved drugs screening against the nsP1 capping enzyme of Venezuelan equine encephalitis virus using an immuno-based assay.
Ferreira-Ramos AS, Li C, Eydoux C, Contreras JM, Morice C, Quérat G, Gigante A, Pérez Pérez MJ, Jung ML, Canard B, Guillemot JC, et al.
Antiviral research (2019) : . 201 011 WB, ELISA
Development of RNA aptamer that inhibits methyltransferase activity of dengue virus.
Jung JI, Han SR, Lee SW
Biotechnology letters (2018) 402: 315-324. 201 011 WB
The NSL complex-mediated nucleosome landscape is required to maintain transcription fidelity and suppression of transcription noise.
Lam KC, Chung HR, Semplicio G, Iyer SS, Gaub A, Bhardwaj V, Holz H, Georgiev P, Akhtar A
Genes & development (2019) 337-8: 452-465. 201 011 IP; tested species: drosophila
Approved drugs screening against the nsP1 capping enzyme of Venezuelan equine encephalitis virus using an immuno-based assay.
Ferreira-Ramos AS, Li C, Eydoux C, Contreras JM, Morice C, Quérat G, Gigante A, Pérez Pérez MJ, Jung ML, Canard B, Guillemot JC, et al.
Antiviral research (2019) : . 201 011 WB, ELISA
Cat. No.: 201 011
Quantity: 100 µg
Price: $415.00
Capping pores of alphavirus nsP1 gate membranous viral replication factories.
Jones R, Bragagnolo G, Arranz R, Reguera J
Nature (2020) : . 201 011 WB
Approved drugs screening against the nsP1 capping enzyme of Venezuelan equine encephalitis virus using an immuno-based assay.
Ferreira-Ramos AS, Li C, Eydoux C, Contreras JM, Morice C, Quérat G, Gigante A, Pérez Pérez MJ, Jung ML, Canard B, Guillemot JC, et al.
Antiviral research (2019) : . 201 011 WB, ELISA
Development of RNA aptamer that inhibits methyltransferase activity of dengue virus.
Jung JI, Han SR, Lee SW
Biotechnology letters (2018) 402: 315-324. 201 011 WB
The NSL complex-mediated nucleosome landscape is required to maintain transcription fidelity and suppression of transcription noise.
Lam KC, Chung HR, Semplicio G, Iyer SS, Gaub A, Bhardwaj V, Holz H, Georgiev P, Akhtar A
Genes & development (2019) 337-8: 452-465. 201 011 IP; tested species: drosophila
Approved drugs screening against the nsP1 capping enzyme of Venezuelan equine encephalitis virus using an immuno-based assay.
Ferreira-Ramos AS, Li C, Eydoux C, Contreras JM, Morice C, Quérat G, Gigante A, Pérez Pérez MJ, Jung ML, Canard B, Guillemot JC, et al.
Antiviral research (2019) : . 201 011 WB, ELISA
Background
Polymerase II transcripts contain a 5´-terminal m7G-cap that is required for the export of these transcripts from the nucleus to the cytoplasm and eucaryotic translation initiation. The Polymerase II transcribed spliceosomal snRNAs U1, U2, U4 and U5 assemble with the eight Sm proteins B/B´, D1, D2, D3, E, F, and G thus forming a core-UsnRNP. The core-UsnRNP is recognized by a methyltransferase that introduces two additional methyl groups to the m7G-cap thus forming the m3G-cap (hypermethylation). The m3G-cap forms one part of the bipartite nuclear localisation signal (NLS) of the UsnRNPs. It is thus necessary for the nuclear re-import of the core-UsnRNPs. Also certain snoRNAs that are involved in the processing of pre-rRNAs contain an m3G-cap.