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m3G-cap, m7G-cap - 201 001

Antibodies against posttranscripitional modifications of RNAs
Monoclonal mouse ascites
Cat. No.: 201 001
Amount: 100 µl
Price: $355.00
Cat. No. 201 001 100 µl ascites, lyophilized. For reconstitution add 100 µl H2O, then aliquot and store at -20°C until use.
Applications WB: not recommended
IP: yes
ICC: yes (see reference)
IHC: not tested yet
IHC-P/FFPE: not tested yet
Clone H20
Subtype IgG1 (κ light chain)
Immunogen Synthetic m3G-cap conjugated to human serum albumin.
Reactivity Reacts with: human, rat, mouse, eukaryotes.
Other species not tested yet.
Specificity Recognizes m3G-cap and m7G-cap.
Remarks

This antibody can be used to detect capped RNAs (e.g. in viruses) or to identify and purify proteins associated with capped RNAs (see reference #2).

Data sheet 201_001.pdf

References for m3G-cap, m7G-cap - 201 001

mRNA Capping by Venezuelan Equine Encephalitis Virus nsP1: Functional Characterization and Implications for Antiviral Research.
Li C, Guillén J, Rabah N, Blanjoie A, Debart F, Vasseur JJ, Canard B, Decroly E, Coutard B
Journal of virology (2015) 8916: 8292-303. 201 001 WB
MAPCap allows high-resolution detection and differential expression analysis of transcription start sites.
Bhardwaj V, Semplicio G, Erdogdu NU, Manke T, Akhtar A
Nature communications (2019) 101: 3219. 201 001 IP; tested species: drosophila
XRN1 stalling in the 5' UTR of Hepatitis C virus and Bovine Viral Diarrhea virus is associated with dysregulated host mRNA stability.
Moon SL, Blackinton JG, Anderson JR, Dozier MK, Dodd BJ, Keene JD, Wilusz CJ, Bradrick SS, Wilusz J
PLoS pathogens (2015) 113: e1004708. 201 001 IP
Microprocessor mediates transcriptional termination of long noncoding RNA transcripts hosting microRNAs.
Dhir A, Dhir S, Proudfoot NJ, Jopling CL
Nature structural & molecular biology (2015) 224: 319-27. 201 001 IP
Noncoding RNAs and LRRFIP1 regulate TNF expression.
Shi L, Song L, Fitzgerald M, Maurer K, Bagashev A, Sullivan KE
Journal of immunology (Baltimore, Md. : 1950) (2014) 1927: 3057-67. 201 001 IP; tested species: human
RNA-methylation-dependent RNA processing controls the speed of the circadian clock.
Fustin JM, Doi M, Yamaguchi Y, Hida H, Nishimura S, Yoshida M, Isagawa T, Morioka MS, Kakeya H, Manabe I, Okamura H, et al.
Cell (2013) 1554: 793-806. 201 001 IP
Stress-induced lncRNAs evade nuclear degradation and enter the translational machinery.
Galipon J, Miki A, Oda A, Inada T, Ohta K
Genes to cells : devoted to molecular & cellular mechanisms (2013) 185: 353-68. 201 001 IP; tested species: fission yeast
The eIF4E-binding protein Eap1p functions in Vts1p-mediated transcript decay.
Rendl LM, Bieman MA, Vari HK, Smibert CA
PloS one (2012) 710: e47121. 201 001 IP
Identification of a cytoplasmic complex that adds a cap onto 5'-monophosphate RNA.
Otsuka Y, Kedersha NL, Schoenberg DR
Molecular and cellular biology (2009) 298: 2155-67. 201 001 IP
Induced ncRNAs allosterically modify RNA-binding proteins in cis to inhibit transcription.
Wang X, Arai S, Song X, Reichart D, Du K, Pascual G, Tempst P, Rosenfeld MG, Glass CK, Kurokawa R
Nature (2008) 4547200: 126-30. 201 001 IP
The interaction between cap-binding complex and RNA export factor is required for intronless mRNA export.
Nojima T, Hirose T, Kimura H, Hagiwara M
The Journal of biological chemistry (2007) 28221: 15645-51. 201 001 IP
The imprinted Air ncRNA is an atypical RNAPII transcript that evades splicing and escapes nuclear export.
Seidl CI, Stricker SH, Barlow DP
The EMBO journal (2006) 2515: 3565-75. 201 001 IP
Snurportin1, an m3G-cap-specific nuclear import receptor with a novel domain structure.
Huber J, Cronshagen U, Kadokura M, Marshallsay C, Wada T, Sekine M, Lührmann R
The EMBO journal (1998) 1714: 4114-26. 201 001 IP
A monoclonal antibody against 2,2,7-trimethylguanosine that reacts with intact, class U, small nuclear ribonucleoproteins as well as with 7-methylguanosine-capped RNAs.
Bochnig P, Reuter R, Bringmann P, Lührmann R
European journal of biochemistry (1987) 1682: 461-7. 201 001 IP
Cat. No.: 201 001
Quantity: 100 µl
Price: $355.00
mRNA Capping by Venezuelan Equine Encephalitis Virus nsP1: Functional Characterization and Implications for Antiviral Research.
Li C, Guillén J, Rabah N, Blanjoie A, Debart F, Vasseur JJ, Canard B, Decroly E, Coutard B
Journal of virology (2015) 8916: 8292-303. 201 001 WB
MAPCap allows high-resolution detection and differential expression analysis of transcription start sites.
Bhardwaj V, Semplicio G, Erdogdu NU, Manke T, Akhtar A
Nature communications (2019) 101: 3219. 201 001 IP; tested species: drosophila
XRN1 stalling in the 5' UTR of Hepatitis C virus and Bovine Viral Diarrhea virus is associated with dysregulated host mRNA stability.
Moon SL, Blackinton JG, Anderson JR, Dozier MK, Dodd BJ, Keene JD, Wilusz CJ, Bradrick SS, Wilusz J
PLoS pathogens (2015) 113: e1004708. 201 001 IP
Microprocessor mediates transcriptional termination of long noncoding RNA transcripts hosting microRNAs.
Dhir A, Dhir S, Proudfoot NJ, Jopling CL
Nature structural & molecular biology (2015) 224: 319-27. 201 001 IP
Noncoding RNAs and LRRFIP1 regulate TNF expression.
Shi L, Song L, Fitzgerald M, Maurer K, Bagashev A, Sullivan KE
Journal of immunology (Baltimore, Md. : 1950) (2014) 1927: 3057-67. 201 001 IP; tested species: human
RNA-methylation-dependent RNA processing controls the speed of the circadian clock.
Fustin JM, Doi M, Yamaguchi Y, Hida H, Nishimura S, Yoshida M, Isagawa T, Morioka MS, Kakeya H, Manabe I, Okamura H, et al.
Cell (2013) 1554: 793-806. 201 001 IP
Stress-induced lncRNAs evade nuclear degradation and enter the translational machinery.
Galipon J, Miki A, Oda A, Inada T, Ohta K
Genes to cells : devoted to molecular & cellular mechanisms (2013) 185: 353-68. 201 001 IP; tested species: fission yeast
The eIF4E-binding protein Eap1p functions in Vts1p-mediated transcript decay.
Rendl LM, Bieman MA, Vari HK, Smibert CA
PloS one (2012) 710: e47121. 201 001 IP
Identification of a cytoplasmic complex that adds a cap onto 5'-monophosphate RNA.
Otsuka Y, Kedersha NL, Schoenberg DR
Molecular and cellular biology (2009) 298: 2155-67. 201 001 IP
Induced ncRNAs allosterically modify RNA-binding proteins in cis to inhibit transcription.
Wang X, Arai S, Song X, Reichart D, Du K, Pascual G, Tempst P, Rosenfeld MG, Glass CK, Kurokawa R
Nature (2008) 4547200: 126-30. 201 001 IP
The interaction between cap-binding complex and RNA export factor is required for intronless mRNA export.
Nojima T, Hirose T, Kimura H, Hagiwara M
The Journal of biological chemistry (2007) 28221: 15645-51. 201 001 IP
The imprinted Air ncRNA is an atypical RNAPII transcript that evades splicing and escapes nuclear export.
Seidl CI, Stricker SH, Barlow DP
The EMBO journal (2006) 2515: 3565-75. 201 001 IP
Snurportin1, an m3G-cap-specific nuclear import receptor with a novel domain structure.
Huber J, Cronshagen U, Kadokura M, Marshallsay C, Wada T, Sekine M, Lührmann R
The EMBO journal (1998) 1714: 4114-26. 201 001 IP
A monoclonal antibody against 2,2,7-trimethylguanosine that reacts with intact, class U, small nuclear ribonucleoproteins as well as with 7-methylguanosine-capped RNAs.
Bochnig P, Reuter R, Bringmann P, Lührmann R
European journal of biochemistry (1987) 1682: 461-7. 201 001 IP
Background
Polymerase II transcripts contain a 5´-terminal m7G-cap that is required for the export of these transcripts from the nucleus to the cytoplasm and eucaryotic translation initiation. The Polymerase II transcribed spliceosomal snRNAs U1, U2, U4 and U5 assemble with the eight Sm proteins B/B´, D1, D2, D3, E, F, and G thus forming a core-UsnRNP. The core-UsnRNP is recognized by a methyltransferase that introduces two additional methyl groups to the m7G-cap thus forming the m3G-cap (hypermethylation). The m3G-cap forms one part of the bipartite nuclear localisation signal (NLS) of the UsnRNPs. It is thus necessary for the nuclear re-import of the core-UsnRNPs. Also certain snoRNAs that are involved in the processing of pre-rRNAs contain an m3G-cap.
Protocols