Synaptic Systems

Cat. No.: 196 002

Amount: 200 µl

Price: $360.00

Cat. No. 196 002	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: 1 : 500 up to 1 : 1000 gallery IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 293 to 384 from mouse PACSIN1 (UniProt Id: Q61644)
Reactivity	Reacts with: rat (Q9Z0W5), mouse (Q61644). Other species not tested yet.
Matching control protein/peptide	196-0P
Data sheet	196_002.pdf

References for PACSIN1 - 196 002

Impaired Presynaptic Function Contributes Significantly to the Pathology of Glycine Receptor Autoantibodies.
Wiessler AL, Zheng F, Werner C, Habib M, Tuzun E, Alzheimer C, Sommer C, Villmann C
Neurology(R) neuroimmunology & neuroinflammation (2025) 122: e200364. 196 002 WB; tested species: mouse

Synaptic AP2 CCV life cycle regulation by the Eps15, ITSN1, Sgip1/AP2, synaptojanin1 interactome.
Mishra R, Sengül GF, Candiello E, Schu P
Scientific reports (2021) 111: 8007. 196 002 WB; tested species: mouse

A novel glycine receptor variant with startle disease affects syndapin I and glycinergic inhibition.
Langlhofer G, Schaefer N, Maric HM, Keramidas A, Zhang Y, Baumann P, Blum R, Breitinger U, Strømgaard K, Schlosser A, Kessels MM, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2020) : . 196 002 WB, ICC; tested species: human,mouse

A local, periactive zone endocytic machinery at photoreceptor synapses in close vicinity to synaptic ribbons.
Wahl S, Katiyar R, Schmitz F
The Journal of neuroscience : the official journal of the Society for Neuroscience (2013) 3325: 10278-300. 196 002 WB, ICC

Native KCC2 interactome reveals PACSIN1 as a critical regulator of synaptic inhibition.
Mahadevan V, Khademullah CS, Dargaei Z, Chevrier J, Uvarov P, Kwan J, Bagshaw RD, Pawson T, Emili A, De Koninck Y, Anggono V, et al.
eLife (2017) 6: . 196 002 IP; tested species: mouse

Dynamin is primed at endocytic sites for ultrafast endocytosis.
Imoto Y, Raychaudhuri S, Ma Y, Fenske P, Sandoval E, Itoh K, Blumrich EM, Matsubayashi HT, Mamer L, Zarebidaki F, Söhl-Kielczynski B, et al.
Neuron (2022) 11017: 2815-2835.e13. 196 002 ICC; tested species: mouse

Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 196 002 ICC, IHC; tested species: mouse,rat

Cat. No.: 196 002

Amount: 200 µl

Price: $360.00

ICC

Background

This protein has been described as PACSIN 1 (for PKC and CK2 substrate in neurons) and syndapin 1 (synaptic, dynamin-associated protein I). It is a multidomain phospho-protein that interacts via its SH3 domain with dynamin 1, synaptojanin, synapsin and N-WASP. Since N-WASP is an activator of the Arp2/3 complex, syndapin may link membrane trafficking and the actin cytoskeleton.
Up to now, 3 isoforms have been described. Syndapin 1 shows a neuronal, syndapin 3 a muscular and syndapin 2 an ubiquitous expression profile.

1 ) Anggono V et al., Nat. Neurosci. (2006)
2 ) Kessels MM et al., J. Biol. Chem. (2006)
3 ) Kessels MM et al., J. Cell. Sci. (2004)
4 ) Modregger J et al., J. Cell. Sci. (2000)
5 ) Qualmann B et al., J. Cell Biol. (2000)
6 ) Qualmann B et al., Mol. Biol. Cell (1999)
7 ) Plomann M et al., Eur. J. Biochem. (1998)

Protocols

ICC

OTHER

Preabsorbtion

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PACSIN1 antibody - 196 002

References for PACSIN1 - 196 002