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Parvalbumin antibody - 195 308

Parvalbumin is calcium binding protein specific for a subpopulation of GABAergic interneurones
Guinea pig monoclonal recombinant IgG
Cat. No.: 195 308
Amount: 50 µg
Price: $415.00
Cat. No. 195 308 50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 500 (AP staining) (see remarks) gallery  
IP: not tested yet
ICC: not tested yet
IHC: 1 : 1000 up to 1 : 2000 gallery  
IHC-P: 1 : 1000 up to 1 : 4000 gallery  
Clone Gp58E1
Subtype IgG2 (κ light chain)
Immunogen Full-length recombinant rat Parvalbumin (UniProt Id: P02625)
Reactivity Reacts with: rat (P02625), mouse (P32848).
No signal: zebrafish.
Other species not tested yet.
Specificity Specific for Parvalbumin.
Remarks

This antibody is a chimeric antibody based on the monoclonal mouse antibody clone 58E1. The constant regions of the heavy and light chains have been replaced by guinea pig specific sequences. Therefore, the antibody can be used with standard anti-guinea pig secondary reagents. The antibody has been expressed in mammalian cells.
WB: Due to the small size of the protein, a tricine gel is recommended. The rabbit polyclonal antiserum (cat. no. 195 002) is more sensitive and recommended for westernblotting.

Data sheet 195_308.pdf
Cat. No.: 195 308
Amount: 50 µg
Price: $415.00
Background
Parvalbumin is a small, acidic, calcium binding protein and belongs to the family of EF hand proteins. The protein is found in skeletal muscle and the brain of vertebrates where it locates to a specific population of GABAergic interneurones. This subset of neurons may contribute to maintaining the balance between excitation and inhibition in the cortex and the hippocampus.