Synaptic Systems - Parvalbumin

Cat. No.: 195 308

Amount: 50 µg

Price: $415.00

Distinct ventral hippocampal inhibitory microcircuits regulating anxiety and fear behaviors.
Li K, Koukoutselos K, Sakaguchi M, Ciocchi S
Nature communications (2024) 151: 8228. 195 308 IHC; tested species: mouse

Pleiotrophin Overexpression Reduces Adolescent Ethanol Consumption and Modulates Ethanol-Induced Glial Responses and Changes in the Perineuronal Nets in the Mouse Hippocampus.
Galán-Llario M, Rodríguez-Zapata M, Fontán-Baselga T, Cañeque-Rufo H, García-Guerra A, Fernández B, Gramage E, Herradón G
CNS neuroscience & therapeutics (2024) 3012: e70159. 195 308 IHC; tested species: mouse

WB

ICC

IHC

IHC-P

Background

Parvalbumin is a small, acidic calcium binding protein and belongs to the family of EF hand proteins. The protein is found in skeletal muscle and the brain of vertebrates where it locates to a specific population of GABAergic interneurons. This subset of neurons may contribute to maintaining the balance between excitation and inhibition in the cortex and the hippocampus.

1 ) Andrioli A et al., Neuroscience (2007)
2 ) Hendrickson A et al., Exp. Eye Res. (2007)
3 ) Fukuda T et al., Neuroscience (2003)
4 ) Kosaka K et al., Exp Brain Res (1994)
5 ) Tolosa de Talamoni N et al., Proc. Natl. Acad. Sci. U.S.A. (1993)
6 ) Kubota Y et al., Neuroreport (1993)

Protocols

WB

ICC

IHC

IHC-P

Other products for this target

Cat. No. 195 308	50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 500 (AP staining) (see remarks) gallery IP: not tested yet ICC: 1 : 500 gallery IHC: 1 : 1000 up to 1 : 2000 gallery IHC-P: 1 : 1000 up to 1 : 4000 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	Gp58E1
Subtype	IgG2 (κ light chain)
Immunogen	Full-length recombinant rat Parvalbumin (UniProt Id: P02625)
Reactivity	Reacts with: rat (P02625), mouse (P32848). No signal: zebrafish. Other species not tested yet.
Remarks	This antibody is a chimeric antibody based on the monoclonal mouse antibody clone 58E1. The constant regions of the heavy and light chains have been replaced by guinea pig specific sequences. Therefore, the antibody can be used with standard anti-guinea pig secondary reagents. The antibody has been expressed in mammalian cells. WB: Due to the small size of the protein, a tricine gel is recommended. The rabbit polyclonal antiserum (cat. no. 195 002) is more sensitive and recommended for westernblotting.
Data sheet	195_308.pdf

Parvalbumin antibody - 195 308

References for Parvalbumin - 195 308