Synaptic Systems - Parvalbumin

Cat. No.: 195 011

Amount: 100 µg

Price: $415.00

Cat. No. 195 011	100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: yes (see remarks) IP: yes ICC: 1 : 500 gallery IHC: 1 : 500 up to 1 : 1000 gallery IHC-P: 1 : 500 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	58E1
Subtype	IgG1 (κ light chain)
Immunogen	Full-length recombinant rat Parvalbumin (UniProt Id: P02625)
Reactivity	Reacts with: rat (P02625), mouse (P32848). No signal: zebrafish. Other species not tested yet.
Matching control protein/peptide	195-0P
Remarks	WB: The rabbit polyclonal antiserum (cat. no. 195 002) is recommended for westernblotting. Due to its small size, a tricine gel should be used.
Data sheet	195_011.pdf

References for Parvalbumin - 195 011

Investigations on the Ability of the Insular Cortex to Process Peripheral Immunosuppression.
Bihorac J, Salem Y, Lückemann L, Schedlowski M, Doenlen R, Engler H, Mark MD, Dombrowski K, Spoida K, Hadamitzky M
Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology (2024) 191: 40. 195 011 IHC; tested species: rat

Adolescent intermittent ethanol exposure decreases perineuronal nets in the hippocampus in a sex dependent manner: Modulation through pharmacological inhibition of RPTPβ/ζ.
Galán-Llario M, Gramage E, García-Guerra A, Torregrosa AB, Gasparyan A, Navarro D, Navarrete F, García-Gutiérrez MS, Manzanares J, Herradón G
Neuropharmacology (2024) 247: 109850. 195 011 IHC; tested species: mouse

Sexually dimorphic role for insular perineuronal nets in aversion-resistant alcohol consumption.
Martins de Carvalho L, Chen H, Sutter M, Lasek AW
Frontiers in psychiatry (2023) 14: 1122423. 195 011 IHC; tested species: mouse

An enriched environment ameliorates the reduction of parvalbumin-positive interneurons in the medial prefrontal cortex caused by maternal separation early in life.
Irie K, Ohta KI, Ujihara H, Araki C, Honda K, Suzuki S, Warita K, Otabi H, Kumei H, Nakamura S, Koyano K, et al.
Frontiers in neuroscience (2023) 17: 1308368. 195 011 IHC; tested species: mouse

The role of α-tubulin tyrosination in controlling the structure and function of hippocampal neurons.
Hosseini S, van Ham M, Erck C, Korte M, Michaelsen-Preusse K
Frontiers in molecular neuroscience (2022) 15: 931859. 195 011 IHC; tested species: mouse

Activity-dependent reconnection of adult-born dentate granule cells in a mouse model of frontotemporal dementia.
Terreros-Roncal J, Flor-García M, Moreno-Jiménez EP, Pallas-Bazarra N, Rábano A, Sah N, van Praag H, Giacomini D, Schinder AF, Ávila J, Llorens-Martín M, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2019) : . 195 011 IHC; tested species: mouse

The transgenic mouse line Igsf9-eGFP allows targeted stimulation of inferior olive efferents.
Pätz C, Brachtendorf S, Eilers J
Journal of neuroscience methods (2018) 296: 84-92. 195 011 IHC; tested species: mouse

Isolated P/Q Calcium Channel Deletion in Layer VI Corticothalamic Neurons Generates Absence Epilepsy.
Bomben VC, Aiba I, Qian J, Mark MD, Herlitze S, Noebels JL
The Journal of neuroscience : the official journal of the Society for Neuroscience (2016) 362: 405-18. 195 011 IHC; tested species: mouse

Mechanisms of Functional Hypoconnectivity in the Medial Prefrontal Cortex of Mecp2 Null Mice.
Sceniak MP, Lang M, Enomoto AC, James Howell C, Hermes DJ, Katz DM
Cerebral cortex (New York, N.Y. : 1991) (2016) 265: 1938-1956. 195 011 IHC; tested species: mouse

Cat. No.: 195 011

Amount: 100 µg

Price: $415.00

ICC

IHC

IHC-P

Background

Parvalbumin is a small, acidic calcium binding protein and belongs to the family of EF hand proteins. The protein is found in skeletal muscle and the brain of vertebrates where it locates to a specific population of GABAergic interneurons. This subset of neurons may contribute to maintaining the balance between excitation and inhibition in the cortex and the hippocampus.

1 ) Andrioli A et al., Neuroscience (2007)
2 ) Hendrickson A et al., Exp. Eye Res. (2007)
3 ) Fukuda T et al., Neuroscience (2003)
4 ) Kosaka K et al., Exp Brain Res (1994)
5 ) Tolosa de Talamoni N et al., Proc. Natl. Acad. Sci. U.S.A. (1993)
6 ) Kubota Y et al., Neuroreport (1993)

Protocols

Standard protocol

ICC

IHC

IHC-P

OTHER

Preabsorbtion

Other products for this target

Parvalbumin antibody - 195 011

References for Parvalbumin - 195 011