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Parvalbumin - 195 008

A calcium binding protein
Monoclonal Recombinant rabbit purified IgG
Cat. No.: 195 008
Amount: 50 µg
Price: 415.00 €
Cat. No. 195 008 50 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.
Applications WB: 1 : 1000 (AP staining) (see remarks) gallery  
IP: not tested yet
ICC: not tested yet
IHC: 1 : 500 gallery  
IHC-P/FFPE: 1 : 100 up to 1 : 500 gallery  
Clone Rb58E1
Subtype IgG1 (κ light chain)
Immunogen Recombinant protein corresponding to AA 1 to 110 from rat Parvalbumin (UniProt Id: P02625)
Reactivity Reacts with: rat (P02625), mouse (P32848).
No signal: zebrafish.
Other species not tested yet.
Specificity Specific for Parvalbumin.
Matching control protein/peptide 195-0P
Remarks

This antibody is a chimeric antibody based on the monoclonal mouse antibody clone 58E1. The constant regions of the heavy and light chains have been replaced by rabbit specific sequences. Therefore, the antibody can be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells.

WB: Due to the small size of the protein, a tricine gel is recommended. The rabbit polyclonal antiserum (cat. no. 195 002) is more sensitive and recommended for westernblotting.

Data sheet 195_008.pdf
Cat. No.: 195 008
Quantity: 50 µg
Price: 415.00 €
Background
Parvalbumin is a small, acidic, calcium binding protein and belongs to the family of EF hand proteins. The protein is found in skeletal muscle and the brain of vertebrates where it locates to a specific population of GABAergic interneurones. This subset of neurons may contribute to maintaining the balance between excitation and inhibition in the cortex and the hippocampus.