mGluR5 antibody - 191 508

mGluRs are metabotropic glutamate receptors involved in excitatory neurotransmission

Rabbit monoclonal recombinant IgG

Cat. No.: 191 508

Amount: 50 µg

Price: $415.00

Cat. No. 191 508	50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not recommended IP: not tested yet ICC: not tested yet IHC: 1 : 500 gallery IHC-P: not recommended IHC-G: 1 : 500 (see remarks) gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Immunohistochemistry on glyoxal fixed (IHC-G) tissue. The tissue is perfused with saline and afterwards immersion fixed with a glyoxal solution. For details of the glyoxal solution, please refer to the remarks section. Immunoreactivity is usually revealed by fluorescence.
Clone	Rb380-035
Subtype	IgG1 (κ light chain)
Immunogen	Full length native human mGluR5 (UniProt Id: P41594)
Reactivity	Reacts with: mouse (Q3UVX5), rat (P31424). Other species not tested yet. Predicted to cross-react with human (P41594) due to high sequence homology.
Remarks	This antibody is a chimeric antibody based on the monoclonal human antibody 380-035. The constant regions of the heavy and light chains have been replaced with rabbit specific sequences. The antibody can therefore be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells. IHC-G: 3% glyoxal, 1% acetic acid, 20% ethanol, in ddH2O, pH 4.2-4.4, according to Richter et al. 2017 is recommended.
Data sheet	191_508.pdf

Cat. No.: 191 508

Amount: 50 µg

Price: $415.00

IHC

IHC-G

Background

Glutamate is the main excitatory neurotransmitter in the vertebrate nervous system and regulates a number of cellular signaling pathways. In addition to the ionotropic glutamate receptors of the NMDA, AMPA and kainate type, eight metabotropic receptors (mGluR1-8) have been described so far (1). This receptor family can be subdivided into three groups (2): Group I receptors (mGluR1, mGluR5) are activated by dihydroxyphenylglycine (DHPG) (3), group II receptors (mGluR2, mGluR3) by dicarboxycyclopropylglycine (DCG-IV), and group III receptors (mGluR4, mGluR6, mGluR7, mGluR8) by aminophosphonobutyrate (L-AP4) (4).

1 ) Shigemoto R et al., J Neurosci (1997)
2 ) Nakanishi S et al., Annu Rev Biophys Biomol Struct (1994)
3 ) Schoepp DD et al., J Neurochem (1994)
4 ) Hayashi Y et al., Nature (1993)

Protocols

IHC

IHC-G