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mGluR5 antibody - 191 508

mGluRs are metabotropic glutamate receptors involved in excitatory neurotransmission
Rabbit monoclonal recombinant IgG
Cat. No.: 191 508
Amount: 50 µg
Price: $415.00
Cat. No. 191 508 50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: not recommended
IP: not tested yet
ICC: not tested yet
IHC: 1 : 500 gallery  
IHC-P: not recommended
IHC-G: 1 : 500 (see remarks) gallery  

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.

Immunohistochemistry on glyoxal fixed (IHC-G) tissue. The tissue is perfused with saline and afterwards immersion fixed with a glyoxal solution. For details of the glyoxal solution, please refer to the remarks section. Immunoreactivity is usually revealed by fluorescence.

Clone Rb380-035
Subtype IgG1 (κ light chain)
Immunogen Full length native human mGluR5 (UniProt Id: P41594)
Remarks

This antibody is a chimeric antibody based on the monoclonal human antibody 380-035. The constant regions of the heavy and light chains have been replaced with rabbit specific sequences. The antibody can therefore be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells.
IHC-G: 3% glyoxal, 1% acetic acid, 20% ethanol, in ddH2O, pH 4.2-4.4, according to Richter et al. 2017 is recommended.

Data sheet 191_508.pdf
Cat. No.: 191 508
Amount: 50 µg
Price: $415.00
Background

Glutamate is the main excitatory neurotransmitter in the vertebrate nervous system and regulates a number of cellular signaling pathways. In addition to the ionotropic glutamate receptors of the NMDA, AMPA and kainate type, eight metabotropic receptors (mGluR1-8) have been described so far (1). This receptor family can be subdivided into three groups (2): Group I receptors (mGluR1, mGluR5) are activated by dihydroxyphenylglycine (DHPG) (3), group II receptors (mGluR2, mGluR3) by dicarboxycyclopropylglycine (DCG-IV), and group III receptors (mGluR4, mGluR6, mGluR7, mGluR8) by aminophosphonobutyrate (L-AP4) (4).