Synaptic Systems - alpha-Protocadherin

Cat. No.: 190 003

Amount: 50 µg

Price: $460.00

Cat. No. 190 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 500 up to 1 : 1000 (AP staining) gallery IP: not tested yet ICC: 1 : 500 gallery IHC: 1 : 500 gallery IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 796 to 946 from rat α-Protocadherin (UniProt Id: Q767I8)
Reactivity	Reacts with: human, rat (Q767I8), mouse (O88689). Other species not tested yet.
Specificity	Detects all α-protocadherins since they share the constant cytoplasmic tail. K.O. validated PubMed: 29439167
Data sheet	190_003.pdf

References for alpha-Protocadherin - 190 003

Combinatorial effects of Alpha- and Gamma-Protocadherins on neuronal survival and dendritic self-avoidance.
Ing-Esteves S, Kostadinov D, Marocha J, Sing AD, Joseph KS, Laboulaye M, Sanes JR, Lefebvre JL
The Journal of neuroscience : the official journal of the Society for Neuroscience (2018) : . 190 003 WB; KO verified; tested species: mouse

Axonal mRNA binding of hnRNP A/B is crucial for axon targeting and maturation of olfactory sensory neurons.
Fukuda N, Fukuda T, Percipalle P, Oda K, Takei N, Czaplinski K, Touhara K, Yoshihara Y, Sasaoka T
Cell reports (2023) 425: 112398. 190 003 IHC; tested species: mouse

Cat. No.: 190 003

Amount: 50 µg

Price: $460.00

ICC

IHC

Background

Cadherins are a complex protein superfamily involved in many cellular processes including cell recognition, cell signaling, cell communication during embryogenesis, and the formation of neural circuits in the central nervous system. Protocadherins constitute the largest group within the cadherin superfamily and can be subdivided into three groups: α-, β- and γ-protocadherins. Genes for these subgroups are organized in closely related gene clusters and encode variable extracellular and transmembrane domains. The short cytosolic tails are constant and shared within one subgroup.

1 ) Bonn S et al., Mol. Cell. Biol. (2007)
2 ) Cronin KD et al., Int. J. Biol. Sci. (2006)
3 ) Triana-Baltzer GB et al., J. Neurobiol. (2006)
4 ) Taguchi Y et al., Mol. Biol. Evol. (2005)
5 ) Wang X et al., Genes Dev. (2002)
6 ) Obata S et al., J. Cell. Sci. (1995)

Protocols

ICC

IHC

Other products for this target

alpha-Protocadherin antibody - 190 003 K.O.

References for alpha-Protocadherin - 190 003