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Veli1/2/3 antibody - 184 003

Velis are multi-adaptor-proteins participating in different synaptic complexes
Rabbit polyclonal purified antibody
Cat. No.: 184 003
Amount: 50 µg
Price: $455.00
Cat. No. 184 003 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: yes
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: 1 : 500 (see remarks) gallery  
IHC-P: not tested yet

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.

Immunogen Recombinant protein corresponding to AA 1 to 232 from rat Velis1 (UniProt Id: Q9Z250)
Reactivity Reacts with: human (O14910, Q9HAP6, Q9NUP9), rat (Q9Z250, Q9Z252, Q792I0), mouse (Q8JZS0, O88951, O88952), zebrafish.
Other species not tested yet.
Specificity Recognizes all three isoforms veli 1, 2 and 3.
Matching control protein/peptide 184-0P
Remarks

IHC: This antibody requires mild fixation.

Data sheet 184_003.pdf

References for Veli1/2/3 - 184 003

Calcium influx and postsynaptic proteins coordinate the dendritic filopodium-spine transition.
Hu HT, Hsueh YP
Developmental neurobiology (2014) 7410: 1011-29. 184 003 ICC, WB
Calcium influx and postsynaptic proteins coordinate the dendritic filopodium-spine transition.
Hu HT, Hsueh YP
Developmental neurobiology (2014) 7410: 1011-29. 184 003 ICC, WB
CASK deletion in intestinal epithelia causes mislocalization of LIN7C and the DLG1/Scrib polarity complex without affecting cell polarity.
Lozovatsky L, Abayasekara N, Piawah S, Walther Z
Molecular biology of the cell (2009) 2021: 4489-99. 184 003 IHC
Cat. No.: 184 003
Amount: 50 µg
Price: $455.00
Calcium influx and postsynaptic proteins coordinate the dendritic filopodium-spine transition.
Hu HT, Hsueh YP
Developmental neurobiology (2014) 7410: 1011-29. 184 003 ICC, WB
Calcium influx and postsynaptic proteins coordinate the dendritic filopodium-spine transition.
Hu HT, Hsueh YP
Developmental neurobiology (2014) 7410: 1011-29. 184 003 ICC, WB
CASK deletion in intestinal epithelia causes mislocalization of LIN7C and the DLG1/Scrib polarity complex without affecting cell polarity.
Lozovatsky L, Abayasekara N, Piawah S, Walther Z
Molecular biology of the cell (2009) 2021: 4489-99. 184 003 IHC
Background

The complex of the multi-adaptor-proteins CASK, Mint and Veli, also referred to as MALS and Lin 7, is conserved across kingdoms and may be involved in localizing synaptic vesicles at synaptic terminals. Three Veli isoforms (1, 2 and 3) with different expression patterns have been described, so far.
Veli proteins have also been suggested to participate in regulating the recruitment of neurotransmitter receptors to the post synaptic density (PSD) by interaction with their PDZ domains.