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GluA - 182 408

A member of a receptor group involved in rapid excitatory neurotransmission in the mammalian CNS
Monoclonal Recombinant rabbit purified IgG
Cat. No.: 182 408
Amount: 50 µg
Price: 415.00 €
Cat. No. 182 408 50 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.
Applications WB: not recommended
IP: not tested yet
ICC: 1 : 200 up to 1 : 500 (see remarks) gallery  
IHC: not tested yet
IHC-P/FFPE: not tested yet
Clone Rb248B7
Subtype IgG1 (κ light chain)
Immunogen Nativ Protein corresponding to AA 22 to 545 from rat GluA2 (UniProt Id: P19491)
Reactivity Reacts with: rat (P19490, P19491, P19492, P19493), mouse (P23818, P23819, Q9Z2W9, Q9Z2W8).
Other species not tested yet.
Specificity Raised against GluA 2 but detects GluA 1, 2, and 3 transfected cells. Due to sequence homology, it likely crossreacts also to GluA 4.
Remarks

This antibody is a chimeric antibody based on the monoclonal mouse antibody clone 248B7. The constant regions of the heavy and light chains have been replaced by rabbit specific sequences. Therefore, the antibody can be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells.

ICC: This antibody is suitable for the surface staining of living cells. After washing cells with bound antibodies they can be fixed and visualized with secondary reagents.

Data sheet 182_408.pdf
Cat. No.: 182 408
Quantity: 50 µg
Price: 415.00 €
Background

Ionotropic glutamate receptors (iGluRs) mediate rapid excitatory neurotransmission in the mammalian CNS. They can be subdivided into three major groups, the AMPA/GluA, NMDA/GluN and kainate/GluK receptors (KARs). mRNAs coding for glutamate receptors are substrates for an adenosine deaminase acting on RNA (ADAR) that increases the diversity of these proteins.
Glutamate receptors of the AMPA subtype are monovalent cation channels and are composed of the four AMPA subunits GluA 1, GluA 2, GluA 3, and GluA 4.

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