Cat. No.: 182 408
Amount: 50 µg
Price:
$415.00
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| Cat. No. 182 408 |
50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze! |
| Applications |
IP: not tested yet ICC: 1 : 200 up to 1 : 500 (see remarks) gallery IHC: not tested yet IHC-P: not tested yet |
| Clone | Rb248B7 |
| Subtype | IgG1 (κ light chain) |
| Immunogen | Nativ Protein corresponding to AA 22 to 545 from rat GluA2 (UniProt Id: P19491) |
| Reactivity |
Reacts with: rat (P19490, P19491, P19492, P19493), mouse (P23818, P23819, Q9Z2W9, Q9Z2W8). Other species not tested yet. |
| Specificity | Raised against GluA 2 but detects GluA 1, 2, and 3 transfected cells. Due to sequence homology, it likely crossreacts also to GluA 4. |
| Remarks |
This antibody is a chimeric antibody based on the monoclonal mouse antibody clone 248B7. The constant regions of the heavy and light chains have been replaced by rabbit specific sequences. Therefore, the antibody can be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells. |
| Data sheet | 182_408.pdf |
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Ionotropic glutamate receptors (iGluRs) mediate rapid excitatory neurotransmission in the mammalian CNS. They can be subdivided into three major groups, the AMPA/GluA, NMDA/GluN and kainate/GluK receptors (KARs). mRNAs coding for glutamate receptors are substrates for an adenosine deaminase acting on RNA (ADAR) that increases the diversity of these proteins.
Glutamate receptors of the AMPA subtype are monovalent cation channels and are composed of the four AMPA subunits GluA 1, GluA 2, GluA 3, and GluA 4.