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Home > Products > 180 313

GluK1 antibody - 180 313

GluKs are ligand-gated ion channel involved in excitatory neurotransmission
Rabbit polyclonal purified antibody
Cat. No.: 180 313
Amount: 50 µg
Price: $375.00
Cat. No. 180 313 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: not tested yet
IP: not tested yet
ICC: not tested yet
IHC: 1 : 5000 up to 1 : 10000 (see remarks) gallery  
IHC-P: not tested yet

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen Synthetic peptide corresponding to AA 899 to 918 from human GluK1 (UniProt Id: P39086)
Reactivity Reacts with: mouse (Q60934).
Other species not tested yet.
Remarks

IHC: For optimal results, mild fixation (immersion fixation with 4% PFA for 15 min) according to Gierke et al. 2023 is recommended.
Data sheet 180_313.pdf

References for GluK1 - 180 313

The absence of functional bassoon at cone photoreceptor ribbon synapses affects signal transmission at Off cone bipolar cell contacts in mouse retina.
Babai N, von Wittgenstein J, Gierke K, Brandstätter JH, Feigenspan A
Acta physiologica (Oxford, England) (2021) 2313: e13584. 180 313 IHC; tested species: mouse
Cat. No.: 180 313
Amount: 50 µg
Price: $375.00
The absence of functional bassoon at cone photoreceptor ribbon synapses affects signal transmission at Off cone bipolar cell contacts in mouse retina.
Babai N, von Wittgenstein J, Gierke K, Brandstätter JH, Feigenspan A
Acta physiologica (Oxford, England) (2021) 2313: e13584. 180 313 IHC; tested species: mouse
IHC
Background

Ionotropic glutamate receptors (iGluRs) mediate rapid excitatory neurotransmission in the mammalian CNS. They can be subdivided into three major groups, the AMPA/GluA, NMDA/GluN, and kainate/GluK receptors (KARs) (1, 2).
mRNAs coding for glutamate receptors are substrates for an adenosine deaminase acting on RNA (ADAR) that increases the diversity of these proteins (3). KARs can be found at pre- and postsynaptic sites and are composed of five different subunits: GluK1, GluK2 and GluK3 can form homomeric receptors whereas GluK4 and GluK5 form heteromeric receptors with GluK1-3 (4).