|Cat. No. 179 002||200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.|
WB: 1 : 1000 (AP staining) gallery
IP: not tested yet
ICC: 1 : 100 up to 1 : 500 gallery
IHC: not tested yet
IHC-P/FFPE: not tested yet
|Immunogen||Recombinant protein corresponding to AA 2 to 155 from rat GOSR1 (UniProt Id: Q62931)|
Reacts with: human (O95249), rat (Q62931), mouse (O88630).
Other species not tested yet.
|Specificity||Specific for GOSR 1.|
|Matching control protein/peptide||179-0P|
GOSR 1 also known as GOS 28 and GS 28 is a 28 kDa membrane protein on the surface of the Golgi compartment. It has been shown to serve as a vSNARE in ER to Golgi transport.
In co-immunoprecipitation studies GOSR 1 was identified as a member of a SNARE complex consisting of syntaxin 5, membrin (GS 27 GOSR 2, GOS 27), rbet1 and rsec22. A more detailed analysis revealed two subcomplexes within this complex. One contains syntaxin 5 (mainly the shorter 35 kDa variant) and GOSR 1 whereas the other is composed of syntaxin 5 (35 and 42 kDa variant), membrin, rsec22 and rbet1.
It has been suggested that theses complexes mediate the fusion of ER-derived vesicles with vesicular tubular clusters (VTC), and the fusion of VTCs to form the cis-Golgi compartment.