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Myobrevin - 176 003

A VAMP isoform expressed preferentially in skeletal muscle and heart
Polyclonal rabbit purified antibody
Cat. No.: 176 003
Amount: 50 µg
Price: $370.00
Cat. No. 176 003 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Applications WB: 1 : 1000 up to 1 : 5000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 1000 gallery  
IHC: yes
IHC-P/FFPE: 1 : 200 gallery  
Immunogen Recombinant protein corresponding to AA 1 to 70 from mouse Myobrevin (UniProt Id: Q9Z2P8)
Reactivity Reacts with: mouse (Q9Z2P8).
No signal: rat.
Other species not tested yet.
Specificity Specific for myobrevin. K.O. PubMed: 29330887
Matching control protein/peptide 176-0P
Data sheet 176_003.pdf

References for Myobrevin - 176 003

Characterisation of GLUT4 trafficking in HeLa cells: comparable kinetics and orthologous trafficking mechanisms to 3T3-L1 adipocytes.
Morris S, Geoghegan ND, Sadler JBA, Koester AM, Black HL, Laub M, Miller L, Heffernan L, Simpson JC, Mastick CC, Cooper J, et al.
PeerJ (2020) 8: e8751. 176 003 WB; tested species: mouse
Cardiac SNARE Expression in Health and Disease.
Bowman PRT, Smith GL, Gould GW
Frontiers in endocrinology (2019) 10: 881. 176 003 WB; tested species: mouse
Loss of VAMP5 in mice results in duplication of the ureter and insufficient expansion of the lung.
Ikezawa M, Tajika Y, Ueno H, Murakami T, Inoue N, Yorifuji H
Developmental dynamics : an official publication of the American Association of Anatomists (2018) : . 176 003 WB, IHC; KO verified; tested species: mouse
Characterization of VAMP isoforms in 3T3-L1 adipocytes: implications for GLUT4 trafficking.
Sadler JB, Bryant NJ, Gould GW
Molecular biology of the cell (2015) 263: 530-6. 176 003 WB
Vesicular transport system in myotubes: ultrastructural study and signposting with vesicle-associated membrane proteins.
Tajika Y, Takahashi M, Khairani AF, Ueno H, Murakami T, Yorifuji H
Histochemistry and cell biology (2014) 1414: 441-54. 176 003 WB, ICC; tested species: mouse
The localization of VAMP5 in skeletal and cardiac muscle.
Takahashi M, Tajika Y, Khairani AF, Ueno H, Murakami T, Yorifuji H
Histochemistry and cell biology (2013) 1394: 573-82. 176 003 WB, IHC
Effects of contraction on localization of GLUT4 and v-SNARE isoforms in rat skeletal muscle.
Rose AJ, Jeppesen J, Kiens B, Richter EA
American journal of physiology. Regulatory, integrative and comparative physiology (2009) 2975: R1228-37. 176 003 WB
Vesicular transport system in myotubes: ultrastructural study and signposting with vesicle-associated membrane proteins.
Tajika Y, Takahashi M, Khairani AF, Ueno H, Murakami T, Yorifuji H
Histochemistry and cell biology (2014) 1414: 441-54. 176 003 WB, ICC; tested species: mouse
Loss of VAMP5 in mice results in duplication of the ureter and insufficient expansion of the lung.
Ikezawa M, Tajika Y, Ueno H, Murakami T, Inoue N, Yorifuji H
Developmental dynamics : an official publication of the American Association of Anatomists (2018) : . 176 003 WB, IHC; KO verified; tested species: mouse
The localization of VAMP5 in skeletal and cardiac muscle.
Takahashi M, Tajika Y, Khairani AF, Ueno H, Murakami T, Yorifuji H
Histochemistry and cell biology (2013) 1394: 573-82. 176 003 WB, IHC
Cat. No.: 176 003
Quantity: 50 µg
Price: $370.00
Characterisation of GLUT4 trafficking in HeLa cells: comparable kinetics and orthologous trafficking mechanisms to 3T3-L1 adipocytes.
Morris S, Geoghegan ND, Sadler JBA, Koester AM, Black HL, Laub M, Miller L, Heffernan L, Simpson JC, Mastick CC, Cooper J, et al.
PeerJ (2020) 8: e8751. 176 003 WB; tested species: mouse
Cardiac SNARE Expression in Health and Disease.
Bowman PRT, Smith GL, Gould GW
Frontiers in endocrinology (2019) 10: 881. 176 003 WB; tested species: mouse
Loss of VAMP5 in mice results in duplication of the ureter and insufficient expansion of the lung.
Ikezawa M, Tajika Y, Ueno H, Murakami T, Inoue N, Yorifuji H
Developmental dynamics : an official publication of the American Association of Anatomists (2018) : . 176 003 WB, IHC; KO verified; tested species: mouse
Characterization of VAMP isoforms in 3T3-L1 adipocytes: implications for GLUT4 trafficking.
Sadler JB, Bryant NJ, Gould GW
Molecular biology of the cell (2015) 263: 530-6. 176 003 WB
Vesicular transport system in myotubes: ultrastructural study and signposting with vesicle-associated membrane proteins.
Tajika Y, Takahashi M, Khairani AF, Ueno H, Murakami T, Yorifuji H
Histochemistry and cell biology (2014) 1414: 441-54. 176 003 WB, ICC; tested species: mouse
The localization of VAMP5 in skeletal and cardiac muscle.
Takahashi M, Tajika Y, Khairani AF, Ueno H, Murakami T, Yorifuji H
Histochemistry and cell biology (2013) 1394: 573-82. 176 003 WB, IHC
Effects of contraction on localization of GLUT4 and v-SNARE isoforms in rat skeletal muscle.
Rose AJ, Jeppesen J, Kiens B, Richter EA
American journal of physiology. Regulatory, integrative and comparative physiology (2009) 2975: R1228-37. 176 003 WB
Vesicular transport system in myotubes: ultrastructural study and signposting with vesicle-associated membrane proteins.
Tajika Y, Takahashi M, Khairani AF, Ueno H, Murakami T, Yorifuji H
Histochemistry and cell biology (2014) 1414: 441-54. 176 003 WB, ICC; tested species: mouse
Loss of VAMP5 in mice results in duplication of the ureter and insufficient expansion of the lung.
Ikezawa M, Tajika Y, Ueno H, Murakami T, Inoue N, Yorifuji H
Developmental dynamics : an official publication of the American Association of Anatomists (2018) : . 176 003 WB, IHC; KO verified; tested species: mouse
The localization of VAMP5 in skeletal and cardiac muscle.
Takahashi M, Tajika Y, Khairani AF, Ueno H, Murakami T, Yorifuji H
Histochemistry and cell biology (2013) 1394: 573-82. 176 003 WB, IHC
Background
Myobrevin, also known as VAMP 5 belongs to the family of vesicle-associated membrane proteins and has a theoretical molecular weight of 11.4 kDa. Like other VAMP isoforms it is composed of an N-terminal cytoplasmic region and a C-terminal transmembrane domain.
Vamp 5 is preferentially expressed in skeletal muscle and heart tissue and is upregulated during the differentiation of C2C12 cells into myotubes.