Synaptic Systems - Myobrevin

Cat. No.: 176 003

Amount: 50 µg

Price: $380.00

Cat. No. 176 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 up to 1 : 5000 (AP staining) gallery IP: not tested yet ICC: 1 : 1000 gallery IHC: yes IHC-P: 1 : 200 gallery
Immunogen	Recombinant protein corresponding to AA 1 to 70 from mouse Myobrevin (UniProt Id: Q9Z2P8)
Reactivity	Reacts with: mouse (Q9Z2P8). No signal: rat. Other species not tested yet.
Specificity	K.O. validated PubMed: 29330887
Matching control protein/peptide	176-0P
Data sheet	176_003.pdf

Lysosomal exocytosis releases pathogenic α-synuclein species from neurons in synucleinopathy models.
Xie YX, Naseri NN, Fels J, Kharel P, Na Y, Lane D, Burré J, Sharma M
Nature communications (2022) 131: 4918. 176 003 WB; tested species: mouse

Characterisation of GLUT4 trafficking in HeLa cells: comparable kinetics and orthologous trafficking mechanisms to 3T3-L1 adipocytes.
Morris S, Geoghegan ND, Sadler JBA, Koester AM, Black HL, Laub M, Miller L, Heffernan L, Simpson JC, Mastick CC, Cooper J, et al.
PeerJ (2020) 8: e8751. 176 003 WB; tested species: mouse

Cardiac SNARE Expression in Health and Disease.
Bowman PRT, Smith GL, Gould GW
Frontiers in endocrinology (2019) 10: 881. 176 003 WB; tested species: mouse

Loss of VAMP5 in mice results in duplication of the ureter and insufficient expansion of the lung.
Ikezawa M, Tajika Y, Ueno H, Murakami T, Inoue N, Yorifuji H
Developmental dynamics : an official publication of the American Association of Anatomists (2018) : . 176 003 WB, IHC; KO verified; tested species: mouse

Characterization of VAMP isoforms in 3T3-L1 adipocytes: implications for GLUT4 trafficking.
Sadler JB, Bryant NJ, Gould GW
Molecular biology of the cell (2015) 263: 530-6. 176 003 WB

Vesicular transport system in myotubes: ultrastructural study and signposting with vesicle-associated membrane proteins.
Tajika Y, Takahashi M, Khairani AF, Ueno H, Murakami T, Yorifuji H
Histochemistry and cell biology (2014) 1414: 441-54. 176 003 WB, ICC; tested species: mouse

The localization of VAMP5 in skeletal and cardiac muscle.
Takahashi M, Tajika Y, Khairani AF, Ueno H, Murakami T, Yorifuji H
Histochemistry and cell biology (2013) 1394: 573-82. 176 003 WB, IHC

Effects of contraction on localization of GLUT4 and v-SNARE isoforms in rat skeletal muscle.
Rose AJ, Jeppesen J, Kiens B, Richter EA
American journal of physiology. Regulatory, integrative and comparative physiology (2009) 2975: R1228-37. 176 003 WB

Cat. No.: 176 003

Amount: 50 µg

Price: $380.00

ICC

IHC-P

Background

Protocols

Myobrevin (Vamp5) antibody - 176 003 K.O.