Synaptic Systems - Neurexin1

Cat. No.: 175 103

Amount: 50 µg

Price: $385.00

Cat. No. 175 103	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 500 up to 1 : 1000 (AP staining) (see remarks) gallery ICC: not recommended IHC: not recommended IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 1328 to 1421 from rat Neurexin1 (UniProt Id: Q63372)
Reactivity	Reacts with: rat (Q63372), mouse (Q9CS84). Other species not tested yet.
Specificity	Specific for neurexin 1. The epitope is present in α- and β-neurexin 1. K.O. validated PubMed: 40234684
Remarks	WB: To avoid protein aggregation, do not heat samples for SDS-PAGE. Non-boiled samples yield stronger signals.
Data sheet	Datasheet 175_103

References for Neurexin1 - 175 103

Targeted splicing approach for alleviation of a neurexin 1 haploinsufficiency model.
Lu H, Roddick KM, Ge Y, Zuo L, Zhang P, Lam O, Marsh K, Wong ROL, Brown RE, Craig AM
Molecular psychiatry (2025) 309: 4353-4363. 175 103 WB, IP; KO verified; tested species: mouse

Convergent depression of activity-dependent bulk endocytosis in rodent models of autism spectrum disorder.
Bonnycastle K, Nawaz MS, Kind PC, Cousin MA
Molecular autism (2025) 161: 26. 175 103 WB; KD verified; tested species: rat

Neurexin-2: An inhibitory neurexin that restricts excitatory synapse formation in the hippocampus.
Lin PY, Chen LY, Jiang M, Trotter JH, Seigneur E, Südhof TC
Science advances (2023) 91: eadd8856. 175 103 WB; tested species: mouse

Alternative splicing and heparan sulfation converge on neurexin-1 to control glutamatergic transmission and autism-related behaviors.
Lu H, Zuo L, Roddick KM, Zhang P, Oku S, Garden J, Ge Y, Bellefontaine M, Delhaye M, Brown RE, Craig AM, et al.
Cell reports (2023) 427: 112714. 175 103 WB; tested species: rat

Deorphanizing FAM19A proteins as pan-neurexin ligands with an unusual biosynthetic binding mechanism.
Khalaj AJ, Sterky FH, Sclip A, Schwenk J, Brunger AT, Fakler B, Südhof TC
The Journal of cell biology (2020) 2199: . 175 103 WB; tested species: mouse

A rare autism-associated MINT2/APBA2 mutation disrupts neurexin trafficking and synaptic function.
Lin AY, Henry S, Reissner C, Neupert C, Kenny C, Missler M, Beffert U, Ho A
Scientific reports (2019) 91: 6024. 175 103 WB; tested species: mouse

Cat. No.: 175 103

Amount: 50 µg

Price: $385.00

Background

α- and β-neurexins are single pass transmembrane proteins with a short cytoplasmic C-terminus and a long extracellular N-terminal part. In α-neurexins the extracellular sequence is substantially longer than in β-neurexins. Alternative splicing of the N-terminal part even confers more complexity to this protein family suggesting distinct binding partners for the extracellular regions. In contrast, the C-termini are highly conserved in the different isoforms and splice-variants and they share overlapping cytosolic binding partners.
Neurexins are receptor like molecules that form heterologous cell contacts with post-synaptic cell surface proteins at synaptic connections (e.g. β-neurexins with neuroligins). They also serve as receptors for the black widow toxin α-latrotoxin which induces neurotransmitter release.

1 ) Comoletti D et al., Structure (2007)
2 ) Craig AM et al., Curr. Opin. Neurobiol. (2007)
3 ) Chih B et al., Neuron (2006)
4 ) Lisé MF et al., Cell. Mol. Life Sci. (2006)
5 ) Paraoanu LE et al., Neurosci. Lett. (2006)
6 ) Dresbach T et al., Mol. Cell. Neurosci. (2004)
7 ) Comoletti D et al., J. Biol. Chem. (2003)
8 ) Dean C et al., Nat. Neurosci. (2003)
9 ) Tabuchi K et al., Genomics (2002)
10 ) Tobaben S et al., J. Biol. Chem. (2002)

Protocols

Neurexin1 antibody - 175 103 K.O.

References for Neurexin1 - 175 103