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GFAP - 173 208

A glial-specific type-III intermediate filament protein
Monoclonal Recombinant rabbit purified IgG
Cat. No.: 173 208
Amount: 50 µg
Price: 415.00 €
Cat. No. 173 208 50 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.
Applications WB: 1 : 1000 (AP staining) (see remarks) gallery  
IP: not tested yet
ICC: 1 : 1000 gallery  
IHC: 1 : 500 gallery  
IHC-P/FFPE: 1 : 200 up to 1 : 1000 gallery  
Clone Rb186C6
Subtype IgG1 (κ light chain)
Immunogen Recombinant protein corresponding to AA 1 to 432 from human GFAP (UniProt Id: P14136)
Epitop Epitop: AA 1 to 15 from human GFAP (UniProt Id: P14136)
Reactivity Reacts with: human (P14136), rat (P47819), mouse (P03995), cow.
Other species not tested yet.
Specificity Specific for GFAP, detects all isoforms.
Matching control protein/peptide 173-0P
Remarks

This antibody is a chimeric antibody based on the monoclonal mouse antibody clone 186C6. The constant regions of the heavy and light chains have been replaced by rabbit specific sequences. Therefore, the antibody can be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells.

WB: In Western blots, the monoclonal GFAP antibodies are less sensitive than the polyclonal rabbit and Guinea pig GFAP antibodies.

Data sheet 173_208.pdf
Cat. No.: 173 208
Quantity: 50 µg
Price: 415.00 €
Background
Glial fibrillary acidic protein GFAP is a glial-specific member of the intermediate filament protein family. This group comprises celltype-specific filamentous proteins with similar structure and function as scaffold for cytoskeleton assembly and maintenance.
Frequently, neural stem cells also express GFAP. In addition many types of brain tumors, probably derived from astrocytic cells, heavily express GFAP. This protein is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and others.
Point-mutations in the GFAP gene have been correlated to Alexander disease a fatal leukoencephalopathy that leads to the dysmyelination or demyelination of the central nervous system.