Tailor-made Antibodies
and Tools for Life Science

GFAP antibody - 173 208 K.O.

GFAP is an astrocyte-specific type-III intermediate filament protein
Rabbit monoclonal recombinant IgG
Cat. No.: 173 208
Amount: 50 µg
Price: $420.00
Cat. No. 173 208 50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) (see remarks) gallery  
IP: not tested yet
ICC: 1 : 1000 gallery  
IHC: 1 : 500 gallery  
IHC-P: 1 : 200 up to 1 : 1000 gallery  
IHC-G: 1 : 1000 up to 1 : 2000 (see remarks) gallery  
Clone Rb186C6
Subtype IgG1 (κ light chain)
Immunogen full-length recombinant human GFAP (UniProt Id: P14136)
Epitop AA 1 to 15 from human GFAP (UniProt Id: P14136)
Reactivity Reacts with: human (P14136), rat (P47819), mouse (P03995), cow.
Other species not tested yet.
Specificity Specific for GFAP, detects all isoforms. K.O. validated
Matching control protein/peptide 173-0P
Remarks

This antibody is a chimeric antibody based on the monoclonal mouse antibody clone 186C6. The constant regions of the heavy and light chains have been replaced by rabbit specific sequences. Therefore, the antibody can be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells.
WB: In Western blots, the monoclonal GFAP antibodies are less sensitive than the polyclonal rabbit antibody.
IHC-G: Fixation with 3% glyoxal, 1% acetic acid, 20% ethanol in ddH2O according to Richter et al. 2017, or 9% glyoxal, 8% acetic acid in ddH2O according to Konno et al. 2023 are possible.

Data sheet 173_208.pdf
Cat. No.: 173 208
Amount: 50 µg
Price: $420.00