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GFAP - 173 011

A glial-specific type-III intermediate filament protein
Monoclonal mouse purified IgG
Cat. No.: 173 011
Amount: 100 µg
Price: 415.00 €
Cat. No. 173 011 100 µg purified IgG, lyophilized. Azide was added before lyophilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.
Applications WB: 1 : 1000 (AP staining) (see remarks) gallery  
IP: yes
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: 1 : 200 up to 1 : 1000 gallery  
IHC-P/FFPE: 1 : 2000 up to 1 : 4000 gallery  
ELISA: yes (see remarks)
Clone 134B1
Subtype IgG2a (κ light chain)
Immunogen Recombinant protein corresponding to AA 1 to 432 from human GFAP (UniProt Id: P14136)
Epitop Epitop: AA 391 to 405 from human GFAP (UniProt Id: P14136)
Reactivity Reacts with: human (P14136), rat (P47819), mouse (P03995), cow.
No signal: zebrafish.
Other species not tested yet.
Specificity Specific for GFAP isoform 1. K.O.
Matching control protein/peptide 173-0P
Remarks

WB: The monoclonal antibodies are less sensitive compared to the rabbit polyclonal polyclonal (cat. no. 173 002).
ELISA: Suitable as capture antibody for sandwich-ELISA with cat. no. 173 002 or 173 211BT as detector antibody.

Data sheet 173_011.pdf

References for GFAP - 173 011

Chronic Toxoplasma infection is associated with distinct alterations in the synaptic protein composition.
Lang D, Schott BH, van Ham M, Morton L, Kulikovskaja L, Herrera-Molina R, Pielot R, Klawonn F, Montag D, Jänsch L, Gundelfinger ED, et al.
Journal of neuroinflammation (2018) 151: 216. 173 011 WB; tested species: mouse
Simvastatin treatment highlights a new role for the isoprenoid/cholesterol biosynthetic pathway in the modulation of emotional reactivity and cognitive performance in rats.
Segatto M, Manduca A, Lecis C, Rosso P, Jozwiak A, Swiezewska E, Moreno S, Trezza V, Pallottini V
Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology (2014) 394: 841-54. 173 011 WB; tested species: rat
Pyroglutamate amyloid β (Aβ) aggravates behavioral deficits in transgenic amyloid mouse model for Alzheimer disease.
Wittnam JL, Portelius E, Zetterberg H, Gustavsson MK, Schilling S, Koch B, Demuth HU, Blennow K, Wirths O, Bayer TA
The Journal of biological chemistry (2012) 28711: 8154-62. 173 011 IP
BDNF Expression in Cortical GABAergic Interneurons.
Barreda Tomás FJ, Turko P, Heilmann H, Trimbuch T, Yanagawa Y, Vida I, Münster-Wandowski A
International journal of molecular sciences (2020) 215: . 173 011 ICC; tested species: mouse
Multiplexed expansion microscopy of the brain through fluorophore screening.
Min K, Cho I, Choi M, Chang JB
Methods (San Diego, Calif.) (2019) : . 173 011 IHC; tested species: mouse
Increased expression of heme-binding protein 1 early in Alzheimer's disease is linked to neurotoxicity.
Yagensky O, Kohansal-Nodehi M, Gunaseelan S, Rabe T, Zafar S, Zerr I, Härtig W, Urlaub H, Chua JJ
eLife (2019) 8: . 173 011 IHC; tested species: mouse
The intermediate filament protein vimentin is essential for axonotrophic effects of Clostridium botulinum C3 exoenzyme.
Adolf A, Leondaritis G, Rohrbeck A, Eickholt BJ, Just I, Ahnert-Hilger G, Höltje M
Journal of neurochemistry (2016) 1392: 234-244. 173 011 IHC
Human adipose-derived stem cells partially rescue the stroke syndromes by promoting spatial learning and memory in mouse middle cerebral artery occlusion model.
Zhou F, Gao S, Wang L, Sun C, Chen L, Yuan P, Zhao H, Yi Y, Qin Y, Dong Z, Cao L, et al.
Stem cell research & therapy (2015) 6: 92. 173 011 IHC; tested species: mouse
Photoreceptor degeneration in two mouse models for congenital stationary night blindness type 2.
Regus-Leidig H, Atorf J, Feigenspan A, Kremers J, Maw MA, Brandstätter JH
PloS one (2014) 91: e86769. 173 011 IHC
Reduced astrocytic NF-κB activation by laquinimod protects from cuprizone-induced demyelination.
Brück W, Pförtner R, Pham T, Zhang J, Hayardeny L, Piryatinsky V, Hanisch UK, Regen T, van Rossum D, Brakelmann L, Hagemeier K, et al.
Acta neuropathologica (2012) 1243: 411-24. 173 011 IHC
Glial fibrillary acidic protein expression alters astrocytic chemokine release and protects mice from cuprizone-induced demyelination.
Kramann N, Menken L, Pförtner R, Schmid SN, Stadelmann C, Wegner C, Brück W
Glia (2019) : . 173 011 IHC-P; tested species: mouse
Phosphorylation of the amyloid β-peptide at Ser26 stabilizes oligomeric assembly and increases neurotoxicity.
Kumar S, Wirths O, Stüber K, Wunderlich P, Koch P, Theil S, Rezaei-Ghaleh N, Zweckstetter M, Bayer TA, Brüstle O, Thal DR, et al.
Acta neuropathologica (2016) 1314: 525-37. 173 011 IHC-P
The Anti-amyloid Compound DO1 Decreases Plaque Pathology and Neuroinflammation-Related Expression Changes in 5xFAD Transgenic Mice.
Boeddrich A, Babila JT, Wiglenda T, Diez L, Jacob M, Nietfeld W, Huska MR, Haenig C, Groenke N, Buntru A, Blanc E, et al.
Cell chemical biology (2018) : . 173 011 ELISA; tested species: mouse
A DNA-based nano-immunoassay for the label-free detection of glial fibrillary acidic protein in multicell lysates.
Ganau M, Bosco A, Palma A, Corvaglia S, Parisse P, Fruk L, Beltrami AP, Cesselli D, Casalis L, Scoles G
Nanomedicine : nanotechnology, biology, and medicine (2015) 112: 293-300. 173 011 ELISA
Cat. No.: 173 011
Quantity: 100 µg
Price: 415.00 €
Chronic Toxoplasma infection is associated with distinct alterations in the synaptic protein composition.
Lang D, Schott BH, van Ham M, Morton L, Kulikovskaja L, Herrera-Molina R, Pielot R, Klawonn F, Montag D, Jänsch L, Gundelfinger ED, et al.
Journal of neuroinflammation (2018) 151: 216. 173 011 WB; tested species: mouse
Simvastatin treatment highlights a new role for the isoprenoid/cholesterol biosynthetic pathway in the modulation of emotional reactivity and cognitive performance in rats.
Segatto M, Manduca A, Lecis C, Rosso P, Jozwiak A, Swiezewska E, Moreno S, Trezza V, Pallottini V
Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology (2014) 394: 841-54. 173 011 WB; tested species: rat
Pyroglutamate amyloid β (Aβ) aggravates behavioral deficits in transgenic amyloid mouse model for Alzheimer disease.
Wittnam JL, Portelius E, Zetterberg H, Gustavsson MK, Schilling S, Koch B, Demuth HU, Blennow K, Wirths O, Bayer TA
The Journal of biological chemistry (2012) 28711: 8154-62. 173 011 IP
BDNF Expression in Cortical GABAergic Interneurons.
Barreda Tomás FJ, Turko P, Heilmann H, Trimbuch T, Yanagawa Y, Vida I, Münster-Wandowski A
International journal of molecular sciences (2020) 215: . 173 011 ICC; tested species: mouse
Multiplexed expansion microscopy of the brain through fluorophore screening.
Min K, Cho I, Choi M, Chang JB
Methods (San Diego, Calif.) (2019) : . 173 011 IHC; tested species: mouse
Increased expression of heme-binding protein 1 early in Alzheimer's disease is linked to neurotoxicity.
Yagensky O, Kohansal-Nodehi M, Gunaseelan S, Rabe T, Zafar S, Zerr I, Härtig W, Urlaub H, Chua JJ
eLife (2019) 8: . 173 011 IHC; tested species: mouse
The intermediate filament protein vimentin is essential for axonotrophic effects of Clostridium botulinum C3 exoenzyme.
Adolf A, Leondaritis G, Rohrbeck A, Eickholt BJ, Just I, Ahnert-Hilger G, Höltje M
Journal of neurochemistry (2016) 1392: 234-244. 173 011 IHC
Human adipose-derived stem cells partially rescue the stroke syndromes by promoting spatial learning and memory in mouse middle cerebral artery occlusion model.
Zhou F, Gao S, Wang L, Sun C, Chen L, Yuan P, Zhao H, Yi Y, Qin Y, Dong Z, Cao L, et al.
Stem cell research & therapy (2015) 6: 92. 173 011 IHC; tested species: mouse
Photoreceptor degeneration in two mouse models for congenital stationary night blindness type 2.
Regus-Leidig H, Atorf J, Feigenspan A, Kremers J, Maw MA, Brandstätter JH
PloS one (2014) 91: e86769. 173 011 IHC
Reduced astrocytic NF-κB activation by laquinimod protects from cuprizone-induced demyelination.
Brück W, Pförtner R, Pham T, Zhang J, Hayardeny L, Piryatinsky V, Hanisch UK, Regen T, van Rossum D, Brakelmann L, Hagemeier K, et al.
Acta neuropathologica (2012) 1243: 411-24. 173 011 IHC
Glial fibrillary acidic protein expression alters astrocytic chemokine release and protects mice from cuprizone-induced demyelination.
Kramann N, Menken L, Pförtner R, Schmid SN, Stadelmann C, Wegner C, Brück W
Glia (2019) : . 173 011 IHC-P; tested species: mouse
Phosphorylation of the amyloid β-peptide at Ser26 stabilizes oligomeric assembly and increases neurotoxicity.
Kumar S, Wirths O, Stüber K, Wunderlich P, Koch P, Theil S, Rezaei-Ghaleh N, Zweckstetter M, Bayer TA, Brüstle O, Thal DR, et al.
Acta neuropathologica (2016) 1314: 525-37. 173 011 IHC-P
The Anti-amyloid Compound DO1 Decreases Plaque Pathology and Neuroinflammation-Related Expression Changes in 5xFAD Transgenic Mice.
Boeddrich A, Babila JT, Wiglenda T, Diez L, Jacob M, Nietfeld W, Huska MR, Haenig C, Groenke N, Buntru A, Blanc E, et al.
Cell chemical biology (2018) : . 173 011 ELISA; tested species: mouse
A DNA-based nano-immunoassay for the label-free detection of glial fibrillary acidic protein in multicell lysates.
Ganau M, Bosco A, Palma A, Corvaglia S, Parisse P, Fruk L, Beltrami AP, Cesselli D, Casalis L, Scoles G
Nanomedicine : nanotechnology, biology, and medicine (2015) 112: 293-300. 173 011 ELISA
Background
Glial fibrillary acidic protein GFAP is a glial-specific member of the intermediate filament protein family. This group comprises celltype-specific filamentous proteins with similar structure and function as scaffold for cytoskeleton assembly and maintenance.
Frequently, neural stem cells also express GFAP. In addition many types of brain tumors, probably derived from astrocytic cells, heavily express GFAP. This protein is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and others.
Point-mutations in the GFAP gene have been correlated to Alexander disease a fatal leukoencephalopathy that leads to the dysmyelination or demyelination of the central nervous system.