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Home > Products > 173 011

GFAP antibody - 173 011 K.O.

GFAP is an astrocyte-specific type-III intermediate filament protein
Mouse monoclonal purified IgG
Cat. No.: 173 011
Amount: 100 µg
Price: $425.00
Cat. No. 173 011 100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) (see remarks) gallery  
IP: yes
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: 1 : 500 up to 1 : 1000 gallery  
IHC-P: 1 : 2000 up to 1 : 4000 gallery  
Clarity: 1 : 200 video (see remarks)
ELISA: yes (see remarks)
Clone 134B1
Subtype IgG2a (κ light chain)
Immunogen full-length recombinant human GFAP (UniProt Id: P14136)
Epitop AA 391 to 405 from human GFAP (UniProt Id: P14136)
Reactivity Reacts with: human (P14136), rat (P47819), mouse (P03995), cow.
No signal: zebrafish.
Other species not tested yet.
Specificity Specific for GFAP isoform 1 (alpha) K.O. validated
Matching control protein/peptide 173-0P
Remarks

WB: The monoclonal antibodies are less sensitive compared to the rabbit polyclonal polyclonal (cat. no. 173 002).
Clarity: This antibody has been successfully used for CLARITY application in human brain (Woelfle et al., 2023; PMID: 37221592).
ELISA: Suitable as capture antibody for sandwich-ELISA with cat. no. 173 002 or 173 211BT as detector antibody.
Data sheet 173_011.pdf

References for GFAP - 173 011

Homeostatic calcium fluxes, ER calcium release, SOCE, and calcium oscillations in cultured astrocytes are interlinked by a small calcium toolkit.
Schulte A, Bieniussa L, Gupta R, Samtleben S, Bischler T, Doering K, Sodmann P, Rittner H, Blum R
Cell calcium (2022) 101: 102515. 173 011 WB, ICC; tested species: mouse
IL-1β Induced Cytokine Expression by Spinal Astrocytes Can Play a Role in the Maintenance of Chronic Inflammatory Pain.
Gajtkó A, Bakk E, Hegedűs K, Ducza L, Holló K
Frontiers in physiology (2020) 11: 543331. 173 011 WB, ICC; tested species: mouse
Chronic Toxoplasma infection is associated with distinct alterations in the synaptic protein composition.
Lang D, Schott BH, van Ham M, Morton L, Kulikovskaja L, Herrera-Molina R, Pielot R, Klawonn F, Montag D, Jänsch L, Gundelfinger ED, et al.
Journal of neuroinflammation (2018) 151: 216. 173 011 WB; tested species: mouse
Simvastatin treatment highlights a new role for the isoprenoid/cholesterol biosynthetic pathway in the modulation of emotional reactivity and cognitive performance in rats.
Segatto M, Manduca A, Lecis C, Rosso P, Jozwiak A, Swiezewska E, Moreno S, Trezza V, Pallottini V
Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology (2014) 394: 841-54. 173 011 WB; tested species: rat
Cat. No.: 173 011
Amount: 100 µg
Price: $425.00
WB
ICC
IHC
IHC
IHC-P
IHC-P
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