Tailor-made Antibodies
and Tools for Life Science

GFAP antibody - 173 008 K.O.

GFAP is an astrocyte-specific type-III intermediate filament protein
Rabbit monoclonal recombinant IgG
Cat. No.: 173 008
Amount: 50 µg
Price: $420.00
Cat. No. 173 008 50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) (see remarks) gallery  
IP: not tested yet
ICC: 1 : 1000 gallery  
IHC: 1 : 2000 gallery  
IHC-P: 1 : 5000 gallery  
Clone Rb134B1
Subtype IgG1 (κ light chain)
Immunogen full-length recombinant human GFAP (UniProt Id: P14136)
Epitop AA 391 to 405 from human GFAP (UniProt Id: P14136)
Reactivity Reacts with: human (P14136), rat (P47819), mouse (P03995), cow.
No signal: zebrafish.
Other species not tested yet.
Specificity Specific for GFAP isoform 1. K.O. validated
Matching control protein/peptide 173-0P
Remarks

This antibody is a chimeric antibody based on the well known monoclonal mouse antibody clone 134B1. The constant regions of the heavy and light chains have been replaced by rabbit specific sequences. Therefore, the antibody can be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells.
WB: In Western blots, the monoclonal GFAP antibodies are less sensitive than the polyclonal rabbit antibody.

Data sheet 173_008.pdf
Cat. No.: 173 008
Amount: 50 µg
Price: $420.00