|Cat. No. 173 008||50 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.|
WB: 1 : 1000 (AP staining) (see remarks) gallery
IP: not tested yet
ICC: 1 : 1000 gallery
IHC: 1 : 2000 gallery
IHC-P/FFPE: 1 : 5000 gallery
|Subtype||IgG1 (κ light chain)|
|Immunogen||Recombinant protein corresponding to AA 1 to 432 from human GFAP (UniProt Id: P14136)|
Epitop: AA 391 to 405 from human GFAP (UniProt Id: P14136)
Reacts with: human (P14136), rat (P47819), mouse (P03995), cow.
No signal: zebrafish.
Other species not tested yet.
|Specificity||Specific for GFAP isoform 1.|
|Matching control protein/peptide||173-0P|
This antibody is a chimeric antibody based on the well known monoclonal mouse antibody clone 134B1. The constant regions of the heavy and light chains have been replaced by rabbit specific sequences. Therefore, the antibody can be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells.
WB: In Western blots, the monoclonal GFAP antibodies are less sensitive than the polyclonal rabbit and Guinea pig GFAP antibodies.