Synaptic Systems

Cat. No.: 173 008

Amount: 50 µg

Price: $415.00

WB

ICC

IHC

IHC-P

Background

Glial fibrillary acidic protein GFAP is a glial-specific member of the intermediate filament protein family. This group comprises cell type-specific filamentous proteins with similar structure and function as scaffold for cytoskeleton assembly and maintenance.
Frequently, neural stem cells also express GFAP. In addition many types of brain tumors, probably derived from astrocytic cells, heavily express GFAP. This protein is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and others.
Point-mutations in the GFAP gene have been correlated to Alexander disease, a fatal leukoencephalopathy that leads to the dysmyelination or demyelination of the central nervous system.

1 ) Triolo D et al., J. Cell. Sci. (2006)
2 ) Shiihara T et al., J. Neurol. Sci. (2004)
3 ) Eng LF et al., Neurochem. Res. (2000)
4 ) Dyer CA et al., J. Neurosci. Res. (2000)
5 ) Valentino KL et al., Brain Res. (1983)
6 ) Palfreyman JW et al., J. Neurol. Sci. (1979)
7 ) Jacque CM et al., J. Neurol. Sci. (1978)

Protocols

WB

ICC

IHC

IHC-P

OTHER

Preabsorbtion

Other products for this target

Cat. No. 173 008	50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) (see remarks) gallery IP: not tested yet ICC: 1 : 1000 gallery IHC: 1 : 2000 gallery IHC-P: 1 : 5000 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	Rb134B1
Subtype	IgG1 (κ light chain)
Immunogen	full-length recombinant human GFAP (UniProt Id: P14136)
Epitop	AA 391 to 405 from human GFAP (UniProt Id: P14136)
Reactivity	Reacts with: human (P14136), rat (P47819), mouse (P03995), cow. No signal: zebrafish. Other species not tested yet.
Specificity	Specific for GFAP isoform 1. K.O. validated
Matching control protein/peptide	173-0P
Remarks	This antibody is a chimeric antibody based on the well known monoclonal mouse antibody clone 134B1. The constant regions of the heavy and light chains have been replaced by rabbit specific sequences. Therefore, the antibody can be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells. WB: In Western blots, the monoclonal GFAP antibodies are less sensitive than the polyclonal rabbit and Guinea pig GFAP antibodies.
Data sheet	173_008.pdf

GFAP antibody - 173 008 K.O.