Synaptic Systems - Neurofilament H

Cat. No.: 171 102

Amount: 200 µl

Price: $355.00

Cat. No. 171 102	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not recommended IP: not tested yet ICC: 1 : 500 gallery IHC: 1 : 500 gallery IHC-P: 1 : 500 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to residues near the carboxy terminus of mouse Neurofilament H (UniProt Id: P19246)
Reactivity	Reacts with: rat (P16884), mouse (P19246). Other species not tested yet.
Data sheet	171_102.pdf

References for Neurofilament H - 171 102

Neurofilament Levels in Dendritic Spines Associate with Synaptic Status.
Gürth CM, do Rego Barros Fernandes Lima MA, Macarrón Palacios V, Cereceda Delgado AR, Hubrich J, D'Este E
Cells (2023) 126: . 171 102 ICC; tested species: rat

Altered anterograde axonal transport of mitochondria in cultured striatal neurons of a knock-in mouse model of Huntington's disease.
Wu C, Yin H, Fu S, Yoo H, Zhang M, Park H
Biochemical and biophysical research communications (2023) 691: 149246. 171 102 ICC; tested species: human

Cat. No.: 171 102

Amount: 200 µl

Price: $355.00

ICC

IHC

IHC-P

Background

Neurofilaments are exclusively expressed in nerve cells and are the major structural component of large-diameter myelinated axons. They are predominatly composed of three proteins, Neurofilament H, L and M and are among the most highly phosphorylated neuronal proteins.

1 ) Barry DM et al., Exp. Cell Res. (2007)
2 ) de Jong D et al., J. Neurol. Neurosurg. Psychiatry (2007)
3 ) Ge WW et al., Mol. Cell. Neurosci. (2007)
4 ) Porchet R et al., Neuroreport (2003)
5 ) Hof PR et al., J. Comp. Neurol. (1996)
6 ) Takeda S et al., J. Cell Biol. (1994)
7 ) Deng Y et al., FASEB J. (2008)
8 ) Fernandes KJ et al., J. Comp. Neurol. (1999)
9 ) Eaker EY et al., Gastroenterology (1990)
10 ) Liem RK et al., J. Cell Biol. (1978)

Protocols

ICC

IHC

IHC-P

Other products for this target

Neurofilament H antibody - 171 102

References for Neurofilament H - 171 102