Neurofilament L antibody - 171 008

Neurofilaments are major neuronal intermediate filaments

Rabbit monoclonal recombinant IgG

Cat. No.: 171 008

Amount: 50 µg

Price: $415.00

Cat. No. 171 008	50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 up to 1 : 5000 gallery IP: not tested yet ICC: 1 : 500 up to 1 : 1000 gallery IHC: 1 : 1000 gallery IHC-P: 1 : 5000 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	Rb69H10
Subtype	IgG1 (κ light chain)
Immunogen	Recombinant protein corresponding to residues near the carboxy terminus of rat Neurofilament L. (UniProt Id: P19527)
Reactivity	Reacts with: mouse (P08551), rat (P19527). Other species not tested yet.
Remarks	This antibody is a chimeric antibody based on the monoclonal mouse antibody clone 69H10. The constant regions of the heavy and light chains have been replaced by rabbit specific sequences. Therefore, the antibody can be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells.
Data sheet	171_008.pdf

Cat. No.: 171 008

Amount: 50 µg

Price: $415.00

ICC

IHC

IHC-P

Background

Neurofilaments are exclusively expressed in nerve cells and are the major structural component of large-diameter myelinated axons. They are predominatly composed of three proteins, Neurofilament H, L and M and are among the most highly phosphorylated neuronal proteins.

1 ) Barry DM et al., Exp. Cell Res. (2007)
2 ) de Jong D et al., J. Neurol. Neurosurg. Psychiatry (2007)
3 ) Ge WW et al., Mol. Cell. Neurosci. (2007)
4 ) Porchet R et al., Neuroreport (2003)
5 ) Hof PR et al., J. Comp. Neurol. (1996)
6 ) Takeda S et al., J. Cell Biol. (1994)
7 ) Deng Y et al., FASEB J. (2008)
8 ) Fernandes KJ et al., J. Comp. Neurol. (1999)
9 ) Eaker EY et al., Gastroenterology (1990)
10 ) Liem RK et al., J. Cell Biol. (1978)

Protocols

ICC

IHC

IHC-P

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