Tailor-made Antibodies
and Tools for Life Science
quick order | cart
?
|
login
Home|||||Technical Support
Home > Products > 168 103

Rab27B antibody - 168 103 K.O.

Rab27 proteins are members of the Rab family responsible for melanosomal transport and hormone secretion
Rabbit polyclonal purified antibody
Cat. No.: 168 103
Amount: 50 µg
Price: $375.00
Cat. No. 168 103 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: yes
ICC: 1 : 500 gallery  
IHC: 1 : 400 gallery  
IHC-P: 1 : 200 gallery  
Immunogen Recombinant protein corresponding to AA 1 to 218 from human Rab27b (UniProt Id: O00194)
Reactivity Reacts with: rat (Q99P74), mouse (Q99P58), human (O00194).
Other species not tested yet.
Specificity Specific for rab 27B with weak cross reaction to rab 27A. K.O. validated PubMed: 26845357
Matching control protein/peptide 168-1P
Data sheet 168_103.pdf

References for Rab27B - 168 103

zDHHC9 Regulates Cardiomyocyte Rab3a Activity and Atrial Natriuretic Peptide Secretion Through Palmitoylation of Rab3gap1.
Essandoh K, Subramani A, Ferro OA, Teuber JP, Koripella S, Brody MJ
JACC. Basic to translational science (2023) 85: 518-542. 168 103 WB; tested species: mouse
Actions of Rab27B-GTPase on mammalian central excitatory synaptic transmission.
Arias-Hervert ER, Xu N, Njus M, Murphy GG, Hou Y, Williams JA, Lentz SI, Ernst SA, Stuenkel EL
Physiological reports (2020) 89: e14428. 168 103 WB, IHC; KO verified; tested species: mouse
MLKL, the Protein that Mediates Necroptosis, Also Regulates Endosomal Trafficking and Extracellular Vesicle Generation.
Yoon S, Kovalenko A, Bogdanov K, Wallach D
Immunity (2017) 471: 51-65.e7. 168 103 WB; KD verified; tested species: human
Genetic deletion of Rab27B in pancreatic acinar cells affects granules size and has inhibitory effects on amylase secretion.
Hou Y, Ernst SA, Lentz SI, Williams JA
Biochemical and biophysical research communications (2016) 4714: 610-5. 168 103 WB; KO verified
Rab27A Is Present in Mouse Pancreatic Acinar Cells and Is Required for Digestive Enzyme Secretion.
Hou Y, Ernst SA, Stuenkel EL, Lentz SI, Williams JA
PloS one (2015) 105: e0125596. 168 103 WB, IHC
EPI64B acts as a GTPase-activating protein for Rab27B in pancreatic acinar cells.
Hou Y, Chen X, Tolmachova T, Ernst SA, Williams JA
The Journal of biological chemistry (2013) 28827: 19548-57. 168 103 WB
Quantitative analysis of synaptic vesicle Rabs uncovers distinct yet overlapping roles for Rab3a and Rab27b in Ca2+-triggered exocytosis.
Pavlos NJ, Grønborg M, Riedel D, Chua JJ, Boyken J, Kloepper TH, Urlaub H, Rizzoli SO, Jahn R
The Journal of neuroscience : the official journal of the Society for Neuroscience (2010) 3040: 13441-53. 168 103 WB, ICC
Rab27b is Involved in Lysosomal Exocytosis and Proteolipid Protein Trafficking in Oligodendrocytes.
Shen YT, Gu Y, Su WF, Zhong JF, Jin ZH, Gu XS, Chen G
Neuroscience bulletin (2016) 324: 331-40. 168 103 ICC
Quantitative analysis of synaptic vesicle Rabs uncovers distinct yet overlapping roles for Rab3a and Rab27b in Ca2+-triggered exocytosis.
Pavlos NJ, Grønborg M, Riedel D, Chua JJ, Boyken J, Kloepper TH, Urlaub H, Rizzoli SO, Jahn R
The Journal of neuroscience : the official journal of the Society for Neuroscience (2010) 3040: 13441-53. 168 103 WB, ICC
Actions of Rab27B-GTPase on mammalian central excitatory synaptic transmission.
Arias-Hervert ER, Xu N, Njus M, Murphy GG, Hou Y, Williams JA, Lentz SI, Ernst SA, Stuenkel EL
Physiological reports (2020) 89: e14428. 168 103 WB, IHC; KO verified; tested species: mouse
Rab27A Is Present in Mouse Pancreatic Acinar Cells and Is Required for Digestive Enzyme Secretion.
Hou Y, Ernst SA, Stuenkel EL, Lentz SI, Williams JA
PloS one (2015) 105: e0125596. 168 103 WB, IHC
Cat. No.: 168 103
Amount: 50 µg
Price: $375.00
zDHHC9 Regulates Cardiomyocyte Rab3a Activity and Atrial Natriuretic Peptide Secretion Through Palmitoylation of Rab3gap1.
Essandoh K, Subramani A, Ferro OA, Teuber JP, Koripella S, Brody MJ
JACC. Basic to translational science (2023) 85: 518-542. 168 103 WB; tested species: mouse
Actions of Rab27B-GTPase on mammalian central excitatory synaptic transmission.
Arias-Hervert ER, Xu N, Njus M, Murphy GG, Hou Y, Williams JA, Lentz SI, Ernst SA, Stuenkel EL
Physiological reports (2020) 89: e14428. 168 103 WB, IHC; KO verified; tested species: mouse
MLKL, the Protein that Mediates Necroptosis, Also Regulates Endosomal Trafficking and Extracellular Vesicle Generation.
Yoon S, Kovalenko A, Bogdanov K, Wallach D
Immunity (2017) 471: 51-65.e7. 168 103 WB; KD verified; tested species: human
Genetic deletion of Rab27B in pancreatic acinar cells affects granules size and has inhibitory effects on amylase secretion.
Hou Y, Ernst SA, Lentz SI, Williams JA
Biochemical and biophysical research communications (2016) 4714: 610-5. 168 103 WB; KO verified
Rab27A Is Present in Mouse Pancreatic Acinar Cells and Is Required for Digestive Enzyme Secretion.
Hou Y, Ernst SA, Stuenkel EL, Lentz SI, Williams JA
PloS one (2015) 105: e0125596. 168 103 WB, IHC
EPI64B acts as a GTPase-activating protein for Rab27B in pancreatic acinar cells.
Hou Y, Chen X, Tolmachova T, Ernst SA, Williams JA
The Journal of biological chemistry (2013) 28827: 19548-57. 168 103 WB
Quantitative analysis of synaptic vesicle Rabs uncovers distinct yet overlapping roles for Rab3a and Rab27b in Ca2+-triggered exocytosis.
Pavlos NJ, Grønborg M, Riedel D, Chua JJ, Boyken J, Kloepper TH, Urlaub H, Rizzoli SO, Jahn R
The Journal of neuroscience : the official journal of the Society for Neuroscience (2010) 3040: 13441-53. 168 103 WB, ICC
Rab27b is Involved in Lysosomal Exocytosis and Proteolipid Protein Trafficking in Oligodendrocytes.
Shen YT, Gu Y, Su WF, Zhong JF, Jin ZH, Gu XS, Chen G
Neuroscience bulletin (2016) 324: 331-40. 168 103 ICC
Quantitative analysis of synaptic vesicle Rabs uncovers distinct yet overlapping roles for Rab3a and Rab27b in Ca2+-triggered exocytosis.
Pavlos NJ, Grønborg M, Riedel D, Chua JJ, Boyken J, Kloepper TH, Urlaub H, Rizzoli SO, Jahn R
The Journal of neuroscience : the official journal of the Society for Neuroscience (2010) 3040: 13441-53. 168 103 WB, ICC
Actions of Rab27B-GTPase on mammalian central excitatory synaptic transmission.
Arias-Hervert ER, Xu N, Njus M, Murphy GG, Hou Y, Williams JA, Lentz SI, Ernst SA, Stuenkel EL
Physiological reports (2020) 89: e14428. 168 103 WB, IHC; KO verified; tested species: mouse
Rab27A Is Present in Mouse Pancreatic Acinar Cells and Is Required for Digestive Enzyme Secretion.
Hou Y, Ernst SA, Stuenkel EL, Lentz SI, Williams JA
PloS one (2015) 105: e0125596. 168 103 WB, IHC
WB
WB
ICC
IHC
IHC-P
Background
Rab 27 proteins are members of the Rab protein family that belongs to the ras-related superfamily of small monomeric GTPases. These proteins are involved in intracellular fusion reactions of vesicles or organelles with their target membranes. Two Rab 27 isoforms, Rab 27A and 27B, have been described so far.
Mutations in the Rab 27A gene have been shown to be responsible for the Griscelli syndrome characterized by pigment dilution of the hair and an uncontrolled T-lymphocyte and macrophage activation. This disorder is probably due to the dysfunction of melanosomes in melanocytes and lytic granules in CTLs. Additionally Rab 27A is located on mature insulin granules of pancreatic β-cells and is expressed in the pigment epithelium and choriocapillaris of the retina.
In patients who suffer from Griscelli syndrome because of missense mutations in the Rab 27A gene, Rab 27B is upregulated and partially compensates for Rab 27A dysfunction. Rab 27B also regulates amylase secretion in parotid acinar cells.
Recently it has been shown that Rab 27 is also involved in synaptic transmission in C. elegans.
Other products for this target