Cat. No.: 163 008
Amount: 50 µg
Price:
$415.00
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| Cat. No. 163 008 |
50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze! |
| Applications | |
| Clone | Rb109C1 |
| Subtype | IgG1 (κ light chain) |
| Immunogen | Recombinant protein corresponding to residues near the central region of mouse Synaptopodin. (UniProt Id: Q8CC35) |
| Reactivity |
Reacts with: rat (Q9Z327), human (Q8N3V7), mouse (Q8CC35). Other species not tested yet. |
| Specificity | This antibody detects the renal Synpo-long and the neuronal Synpo-short isofoms but is negativ for the T-variant. K.O. validated |
| Remarks |
This antibody is a chimeric antibody based on the monoclonal mouse antibody 109C1. The constant regions of the heavy and light chains have been replaced with rabbit specific sequences. The antibody can therefore be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells. |
| Data sheet | 163_008.pdf |
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Synaptopodin is the founding member of a class of proline-rich actin-associated proteins and has been originally identified in podocytes of renal glomeruli (1, 2). In brain it localizes to the post-synaptic density (PSD) and the spine aparatus (1). In humans, three isoforms of synaptopodin with a distinct expression profile have been identified, neuronal Synpo-short, renal Synpo-long and Synpo-T. All three isoforms interact with alpha-actinin and induce alpha-actinin-induced actin filaments (3).
Synaptopodin deficient mice lack the dendritic spine apparatus and exhibit impaired activity-dependent long-term synaptic plasticity (4).