Synaptic Systems

Cat. No.: 162 304

Amount: 100 µl

Price: $370.00

Cat. No. 162 304	100 µl antiserum, lyophilized. For reconstitution add 100 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes ICC: 1 : 500 up to 1 : 2000 gallery IHC: 1 : 500 gallery IHC-P: 1 : 500 gallery IHC-Fr: 1 : 500 (see remarks) gallery ExM: 1 : 200 (see remarks) gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Immunohistochemistry on fresh frozen (IHC-Fr) cryo-tissue-sections. In contrast to standard PFA perfusion fixed tissues, fresh frozen cryo-tissue-sections can be variably postfixed with alcohols, acetone or PFA. Alcohol or acetone fixation is e.g. of advantage for antigens masked by PFA crosslinking. For recommended postfixation, please refer to the ”Remarks” section. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Expansion Microscopy (ExM) is a sample preparation and imaging method which employs a dense interconnected web of swellable polymer within a biological specimen. A significantly higher effective resolution can be achieved with a standard microscope setup. For additional experimental details, please refer to the Remarks section.
Immunogen	Recombinant protein corresponding to residues near the carboxy terminus of rat Shank3 (UniProt Id: Q9JLU4)
Reactivity	Reacts with: rat (Q9JLU4), mouse (Q4ACU6). Other species not tested yet.
Specificity	Antigen used for immunization is present in all Shank3 isoforms described for rat and in all isoforms described for mouse except Shank3-B and Shank3-C4. K.O. validated PubMed: 33115499
Remarks	IHC-Fr: Fixation with formaldehyde/PFA is recommended ExM: This antibody has been successfully used for the magnified analysis of the proteome (MAP) expansion microscopy method (MAP; Ku et al. 2016. Nature Biotechnology 34:973-981)
Data sheet	162_304.pdf

References for Shank3 - 162 304

SHANK3 Antibody Validation: Differential Performance in Western Blotting, Immunocyto- and Immunohistochemistry.
Lutz AK, Bauer HF, Ioannidis V, Schön M, Boeckers TM
Frontiers in synaptic neuroscience (2022) 14: 890231. 162 304 WB, ICC, IHC; tested species: mouse

A bidirectional switch in the Shank3 phosphorylation state biases synapses toward up- or downscaling.
Wu CH, Tatavarty V, Jean Beltran PM, Guerrero AA, Keshishian H, Krug K, MacMullan MA, Li L, Carr SA, Cottrell JR, Turrigiano GG, et al.
eLife (2022) 11: . 162 304 WB, IP, ICC; tested species: rat

Germline AGO2 mutations impair RNA interference and human neurological development.
Lessel D, Zeitler DM, Reijnders MRF, Kazantsev A, Hassani Nia F, Bartholomäus A, Martens V, Bruckmann A, Graus V, McConkie-Rosell A, McDonald M, et al.
Nature communications (2020) 111: 5797. 162 304 WB, ICC; KD verified; tested species: rat

Targeting of δ-catenin to postsynaptic sites through interaction with the Shank3 N-terminus.
Hassani Nia F, Woike D, Martens V, Klüssendorf M, Hönck HH, Harder S, Kreienkamp HJ
Molecular autism (2020) 111: 85. 162 304 WB; KO verified; tested species: mouse

Chronic Toxoplasma infection is associated with distinct alterations in the synaptic protein composition.
Lang D, Schott BH, van Ham M, Morton L, Kulikovskaja L, Herrera-Molina R, Pielot R, Klawonn F, Montag D, Jänsch L, Gundelfinger ED, et al.
Journal of neuroinflammation (2018) 151: 216. 162 304 WB, IHC; tested species: mouse

Variants in LRRC7 lead to intellectual disability, autism, aggression and abnormal eating behaviors.
Willim J, Woike D, Greene D, Das S, Pfeifer K, Yuan W, Lindsey A, Itani O, Böhme AL, Tibbe D, Hönck HH, et al.
Nature communications (2024) 151: 7909. 162 304 ICC; tested species: rat

Polar lipids modify Alzheimer's Disease pathology by reducing astrocyte pro-inflammatory signaling through platelet-activating factor receptor (PTAFR) modulation.
Hans S, Stanton JE, Sauer AK, Shiels K, Saha SK, Lordan R, Tsoupras A, Zabetakis I, Grabrucker AM
Lipids in health and disease (2024) 231: 113. 162 304 ICC; tested species: rat

Jacob-induced transcriptional inactivation of CREB promotes Aβ-induced synapse loss in Alzheimer's disease.
Grochowska KM, Gomes GM, Raman R, Kaushik R, Sosulina L, Kaneko H, Oelschlegel AM, Yuanxiang P, Reyes-Resina I, Bayraktar G, Samer S, et al.
The EMBO journal (2023) : e112453. 162 304 ICC, IHC; tested species: mouse

Golgi satellites are essential for polysialylation of NCAM and expression of LTP at distal synapses.
Andres-Alonso M, Borgmeyer M, Mirzapourdelavar H, Lormann J, Klein K, Schweizer M, Hoffmeister-Ullerich S, Oelschlegel AM, Dityatev A, Kreutz MR
Cell reports (2023) 427: 112692. 162 304 ICC, IHC; tested species: mouse

Chaperone-mediated autophagy in neuronal dendrites utilizes activity-dependent lysosomal exocytosis for protein disposal.
Grochowska KM, Sperveslage M, Raman R, Failla AV, Głów D, Schulze C, Laprell L, Fehse B, Kreutz MR
Cell reports (2023) 428: 112998. 162 304 ICC; tested species: rat

Multiomics of synaptic junctions reveals altered lipid metabolism and signaling following environmental enrichment.
Borgmeyer M, Coman C, Has C, Schött HF, Li T, Westhoff P, Cheung YFH, Hoffmann N, Yuanxiang P, Behnisch T, Gomes GM, et al.
Cell reports (2021) 371: 109797. 162 304 ICC, IHC; tested species: mouse,rat

The histone demethylase PHF8 regulates astrocyte differentiation and function.
Iacobucci S, Padilla N, Gabrielli M, Navarro C, Lombardi M, Vicioso-Mantis M, Verderio C, de la Cruz X, Martínez-Balbás MA
Development (Cambridge, England) (2021) 14812: . 162 304 ICC; tested species: mouse

Ciliary neuropeptidergic signaling dynamically regulates excitatory synapses in postnatal neocortical pyramidal neurons.
Tereshko L, Gao Y, Cary BA, Turrigiano GG, Sengupta P
eLife (2021) 10: . 162 304 ICC; tested species: rat

Autism-associated SHANK3 missense point mutations impact conformational fluctuations and protein turnover at synapses.
Bucher M, Niebling S, Han Y, Molodenskiy D, Hassani Nia F, Kreienkamp HJ, Svergun D, Kim E, Kostyukova AS, Kreutz MR, Mikhaylova M, et al.
eLife (2021) 10: . 162 304 ICC; tested species: rat

USP8 Deubiquitinates SHANK3 to Control Synapse Density and SHANK3 Activity-dependent Protein Levels.
Kerrisk Campbell M, Sheng M
The Journal of neuroscience : the official journal of the Society for Neuroscience (2018) : . 162 304 ICC; tested species: rat

A septal-ventral tegmental area circuit drives exploratory behavior.
Mocellin P, Barnstedt O, Luxem K, Kaneko H, Vieweg S, Henschke JU, Dalügge D, Fuhrmann F, Karpova A, Pakan JMP, Kreutz MR, et al.
Neuron (2024) : . 162 304 IHC; tested species: mouse

Multisensory gamma stimulation mitigates the effects of demyelination induced by cuprizone in male mice.
Rodrigues-Amorim D, Bozzelli PL, Kim T, Liu L, Gibson O, Yang CY, Murdock MH, Galiana-Melendez F, Schatz B, Davison A, Islam MR, et al.
Nature communications (2024) 151: 6744. 162 304 IHC; tested species: mouse

A High-Resolution Method for Quantitative Molecular Analysis of Functionally Characterized Individual Synapses.
Holderith N, Heredi J, Kis V, Nusser Z
Cell reports (2020) 324: 107968. 162 304 IHC; tested species: rat

Cat. No.: 162 304

Amount: 100 µl

Price: $370.00

ICC

IHC

IHC-P

IHC-Fr

ExM

Background

Shank1, 2 and 3 are major proteins of the postsynaptic density (PSD). They are composed of several protein-protein interaction domains like PDZ-, homer- and ABP1-binding domains which allow them to crosslink ionotopic and metabotropic glutamate receptor complexes with each other and to the actin-cytoskeleton.

1 ) Sala C et al., J. Neurosci. (2005)
2 ) Roussignol G et al., J. Neurosci. (2005)
3 ) Quitsch A et al., J. Neurosci. (2005)
4 ) Qualmann B et al., J. Neurosci. (2004)
5 ) Im YJ et al., J. Biol. Chem. (2003)
6 ) Boeckers TM et al., J. Neurochem. (2002)
7 ) Sala C et al., Neuron (2001)
8 ) Tobaben S et al., J. Biol. Chem. (2000)
9 ) Sheng M et al., J. Cell. Sci. (2000)
10 ) Naisbitt S et al., Neuron (1999)

Protocols

Standard protocol

ICC

IHC

IHC-P

IHC-Fr

Other products for this target

Shank3 antibody - 162 304 K.O.

References for Shank3 - 162 304