Synaptic Systems

Cat. No.: 162 302

Amount: 200 µl

Price: $360.00

Cat. No. 162 302	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: 1 : 500 gallery IHC: 1 : 500 (see remarks) gallery IHC-P: not tested yet IHC-Fr: 1 : 500 (see remarks) gallery EM: yes
Immunogen	Recombinant protein corresponding to residues near the carboxy terminus of rat Shank3 (UniProt Id: Q9JLU4)
Reactivity	Reacts with: rat (Q9JLU4), mouse (Q4ACU6). Other species not tested yet.
Specificity	K.O. validated
Remarks	IHC: This antibody requires mild fixation. IHC-Fr: Fixation with PFA/formaldehyde is recommended
Data sheet	162_302.pdf

Early developmental deletion of forebrain Ank2 causes seizure-related phenotypes by reshaping the synaptic proteome.
Yoon S, Santos MD, Forrest MP, Pratt CP, Khalatyan N, Mohler PJ, Savas JN, Penzes P
Cell reports (2023) 427: 112784. 162 302 WB, IP; tested species: mouse

SHANK3 Antibody Validation: Differential Performance in Western Blotting, Immunocyto- and Immunohistochemistry.
Lutz AK, Bauer HF, Ioannidis V, Schön M, Boeckers TM
Frontiers in synaptic neuroscience (2022) 14: 890231. 162 302 WB, ICC, IHC; tested species: mouse

SREBP2 gene therapy targeting striatal astrocytes ameliorates Huntington's disease phenotypes.
Birolini G, Verlengia G, Talpo F, Maniezzi C, Zentilin L, Giacca M, Conforti P, Cordiglieri C, Caccia C, Leoni V, Taroni F, et al.
Brain : a journal of neurology (2021) 14410: 3175-3190. 162 302 WB; tested species: mouse

Zinc Stabilizes Shank3 at the Postsynaptic Density of Hippocampal Synapses.
Tao-Cheng JH, Toy D, Winters CA, Reese TS, Dosemeci A
PloS one (2016) 115: e0153979. 162 302 WB, EM

Reduced Glutamate Release in Adult BTBR Mouse Model of Autism Spectrum Disorder.
Wei H, Ma Y, Ding C, Jin G, Liu J, Chang Q, Hu F, Yu L
Neurochemical research (2016) 4111: 3129-3137. 162 302 WB, EM

Inhibition of IL-6 trans-signaling in the brain increases sociability in the BTBR mouse model of autism.
Wei H, Ma Y, Liu J, Ding C, Jin G, Wang Y, Hu F, Yu L
Biochimica et biophysica acta (2016) 186210: 1918-25. 162 302 WB, IHC; tested species: mouse

Effects of trace metal profiles characteristic for autism on synapses in cultured neurons.
Hagmeyer S, Mangus K, Boeckers TM, Grabrucker AM
Neural plasticity (2015) 2015: 985083. 162 302 ICC, WB

REST-Governed Gene Expression Profiling in a Neuronal Cell Model Reveals Novel Direct and Indirect Processes of Repression and Up-Regulation.
Garcia-Manteiga JM, Bonfiglio S, Folladori L, Malosio ML, Lazarevic D, Stupka E, Cittaro D, Meldolesi J
Frontiers in cellular neuroscience (2015) 9: 438. 162 302 WB