Synaptic Systems

Cat. No.: 162 204

Amount: 100 µl

Price: $400.00

Cat. No. 162 204	100 µl antiserum, lyophilized. For reconstitution add 100 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 up to 1 : 5000 (AP staining) gallery IP: not tested yet ICC: 1 : 1000 up to 1 : 2000 gallery IHC: 1 : 500 gallery IHC-P: 1 : 500 ExM: 1 : 200 (see remarks) gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Expansion Microscopy (ExM) is a sample preparation and imaging method which employs a dense interconnected web of swellable polymer within a biological specimen. A significantly higher effective resolution can be achieved with a standard microscope setup. For additional experimental details, please refer to the Remarks section.
Immunogen	Recombinant protein corresponding to residues near the carboxy terminus of rat Shank2 (UniProt Id: Q9QX74)
Reactivity	Reacts with: rat (Q9QX74), mouse (Q80Z38), human (Q9UPX8). Other species not tested yet.
Specificity	K.O. validated PubMed: 29970987
Remarks	ExM: This antibody has been successfully used for the magnified analysis of the proteome (MAP) expansion microscopy method (MAP; Ku et al. 2016. Nature Biotechnology 34:973-981)
Data sheet	162_204.pdf

References for Shank2 - 162 204

Manipulation of DHPS activity affects dendritic morphology and expression of synaptic proteins in primary rat cortical neurons.
Cavalli P, Raffauf A, Passarella S, Helmuth M, Dieterich DC, Landgraf P
Frontiers in cellular neuroscience (2024) 18: 1465011. 162 204 WB; tested species: rat

Dendritic spine morphology and memory formation depend on postsynaptic Caskin proteins.
Bencsik N, Pusztai S, Borbély S, Fekete A, Dülk M, Kis V, Pesti S, Vas V, Szűcs A, Buday L, Schlett K, et al.
Scientific reports (2019) 91: 16843. 162 204 WB, IP, ICC; tested species: mouse

Rasopathy-Associated Mutation Ptpn11D61Y has Age-Dependent Effect on Synaptic Vesicle Recycling.
Guhathakurta D, Selzam F, Petrušková A, Weiss EM, Akdaş EY, Montenegro-Venegas C, Zenker M, Fejtová A
Cellular and molecular neurobiology (2024) 441: 77. 162 204 ICC; tested species: mouse

An intellectual-disability-associated mutation of the transcriptional regulator NACC1 impairs glutamatergic neurotransmission.
Daniel JA, Elizarova S, Shaib AH, Chouaib AA, Magnussen HM, Wang J, Brose N, Rhee J, Tirard M
Frontiers in molecular neuroscience (2023) 16: 1115880. 162 204 ICC; tested species: mouse

Quantifying molecular aggregation by super resolution microscopy within an excitatory synapse from mouse hippocampal neurons.
Kedia S, Ramanan N, Nair D
STAR protocols (2021) 22: 100470. 162 204 ICC; tested species: mouse

Protein kinase D promotes activity-dependent AMPA receptor endocytosis in hippocampal neurons.
Oueslati Morales CO, Ignácz A, Bencsik N, Sziber Z, Rátkai AE, Lieb WS, Eisler SA, Szűcs A, Schlett K, Hausser A
Traffic (Copenhagen, Denmark) (2021) 2212: 454-470. 162 204 ICC; tested species: mouse

Differential Scaling of Synaptic Molecules within Functional Zones of an Excitatory Synapse during Homeostatic Plasticity.
Venkatesan S, Subramaniam S, Rajeev P, Chopra Y, Jose M, Nair D
eNeuro (2020) 72: . 162 204 ICC; tested species: rat

An Autaptic Culture System for Standardized Analyses of iPSC-Derived Human Neurons.
Rhee HJ, Shaib AH, Rehbach K, Lee C, Seif P, Thomas C, Gideons E, Guenther A, Krutenko T, Hebisch M, Peitz M, et al.
Cell reports (2019) 277: 2212-2228.e7. 162 204 ICC; tested species: human

Serine-Arginine Protein Kinase SRPK2 Modulates the Assembly of the Active Zone Scaffolding Protein CAST1/ERC2.
Arancibia D, Lira M, Cruz Y, Barrera DP, Montenegro-Venegas C, Godoy JA, Garner CC, Inestrosa NC, Gundelfinger ED, Zamorano P, Torres VI, et al.
Cells (2019) 811: . 162 204 ICC; tested species: rat

The Exocyst Component Exo70 Modulates Dendrite Arbor Formation, Synapse Density, and Spine Maturation in Primary Hippocampal Neurons.
Lira M, Arancibia D, Orrego PR, Montenegro-Venegas C, Cruz Y, García J, Leal-Ortiz S, Godoy JA, Gundelfinger ED, Inestrosa NC, Garner CC, et al.
Molecular neurobiology (2018) : . 162 204 ICC; tested species: rat

Photoproximity labeling of endogenous receptors in the live mouse brain in minutes.
Takato M, Sakamoto S, Nonaka H, Tanimura Valor FY, Tamura T, Hamachi I
Nature chemical biology (2024) : . 162 204 IHC; tested species: mouse

A thalamic-primary auditory cortex circuit mediates resilience to stress.
Li HY, Zhu MZ, Yuan XR, Guo ZX, Pan YD, Li YQ, Zhu XH
Cell (2023) 1867: 1352-1368.e18. 162 204 IHC; tested species: mouse

Dietary zinc supplementation rescues fear-based learning and synaptic function in the Tbr1+/- mouse model of autism spectrum disorders.
Lee K, Jung Y, Vyas Y, Skelton I, Abraham WC, Hsueh YP, Montgomery JM
Molecular autism (2022) 131: 13. 162 204 IHC; tested species: mouse

Ketogenic diet uncovers differential metabolic plasticity of brain cells.
Düking T, Spieth L, Berghoff SA, Piepkorn L, Schmidke AM, Mitkovski M, Kannaiyan N, Hosang L, Scholz P, Shaib AH, Schneider LV, et al.
Science advances (2022) 837: eabo7639. 162 204 IHC; tested species: mouse

Alteration in synaptic nanoscale organization dictates amyloidogenic processing in Alzheimer's disease.
Kedia S, Ramakrishna P, Netrakanti PR, Singh N, Sisodia SS, Jose M, Kumar S, Mahadevan A, Ramanan N, Nadkarni S, Nair D, et al.
iScience (2021) 241: 101924. 162 204 IHC; tested species: mouse

Local Efficacy of Glutamate Uptake Decreases with Synapse Size.
Herde MK, Bohmbach K, Domingos C, Vana N, Komorowska-Müller JA, Passlick S, Schwarz I, Jackson CJ, Dietrich D, Schwarz MK, Henneberger C, et al.
Cell reports (2020) 3212: 108182. 162 204 IHC; tested species: mouse

Inositol polyphosphate multikinase mediates extinction of fear memory.
Park J, Longo F, Park SJ, Lee S, Bae M, Tyagi R, Han JH, Kim S, Santini E, Klann E, Snyder SH, et al.
Proceedings of the National Academy of Sciences of the United States of America (2019) : . 162 204 IHC; tested species: mouse

Localization of group II and III metabotropic glutamate receptors at pre- and postsynaptic sites of inner hair cell ribbon synapses.
Klotz L, Wendler O, Frischknecht R, Shigemoto R, Schulze H, Enz R
FASEB journal : official publication of the Federation of American Societies for Experimental Biology (2019) : fj201901543R. 162 204 IHC; tested species: mouse

Cell-Type-Specific Shank2 Deletion in Mice Leads to Differential Synaptic and Behavioral Phenotypes.
Kim R, Kim J, Chung C, Ha S, Lee S, Lee E, Yoo YE, Kim W, Shin W, Kim E
The Journal of neuroscience : the official journal of the Society for Neuroscience (2018) 3817: 4076-4092. 162 204 IHC; tested species: mouse

Shank2 Deletion in Parvalbumin Neurons Leads to Moderate Hyperactivity, Enhanced Self-Grooming and Suppressed Seizure Susceptibility in Mice.
Lee S, Lee E, Kim R, Kim J, Lee S, Park H, Yang E, Kim H, Kim E
Frontiers in molecular neuroscience (2018) 11: 209. 162 204 IHC; KO verified; tested species: mouse

APP accumulates with presynaptic proteins around amyloid plaques: A role for presynaptic mechanisms in Alzheimer's disease?
Jordà-Siquier T, Petrel M, Kouskoff V, Smailovic U, Cordelières F, Frykman S, Müller U, Mulle C, Barthet G
Alzheimer's & dementia : the journal of the Alzheimer's Association (2022) : . 162 204 IHC-P; tested species: human

Distinctive alteration of presynaptic proteins in the outer molecular layer of the dentate gyrus in Alzheimer's disease.
Haytural H, Jordà-Siquier T, Winblad B, Mulle C, Tjernberg LO, Granholm AC, Frykman S, Barthet G
Brain communications (2021) 32: fcab079. 162 204 IHC-P; tested species: human

Mapping proteomic composition of excitatory postsynaptic sites in the cerebellar cortex.
Robinson K, Delhaye M, Craig AM
Frontiers in molecular neuroscience (2024) 17: 1381534. 162 204 ExM; tested species: mouse

Heat denaturation enables multicolor X10-STED microscopy.
Saal KA, Shaib AH, Mougios N, Crzan D, Opazo F, Rizzoli SO
Scientific reports (2023) 131: 5366. 162 204 ExM; tested species: rat

Cat. No.: 162 204

Amount: 100 µl

Price: $400.00

A thalamic-primary auditory cortex circuit mediates resilience to stress.
Li HY, Zhu MZ, Yuan XR, Guo ZX, Pan YD, Li YQ, Zhu XH
Cell (2023) 1867: 1352-1368.e18. 162 204 IHC; tested species: mouse

Heat denaturation enables multicolor X10-STED microscopy.
Saal KA, Shaib AH, Mougios N, Crzan D, Opazo F, Rizzoli SO
Scientific reports (2023) 131: 5366. 162 204 ExM; tested species: rat

ICC

IHC

ExM

Background

Shank1, 2 and 3 are major proteins of the postsynaptic density (PSD). They are composed of several protein-protein interaction domains like PDZ-, homer- and ABP1-binding domains which allow them to crosslink ionotopic and metabotropic glutamate receptor complexes with each other and to the actin-cytoskeleton.

1 ) Sala C et al., J. Neurosci. (2005)
2 ) Roussignol G et al., J. Neurosci. (2005)
3 ) Quitsch A et al., J. Neurosci. (2005)
4 ) Qualmann B et al., J. Neurosci. (2004)
5 ) Im YJ et al., J. Biol. Chem. (2003)
6 ) Boeckers TM et al., J. Neurochem. (2002)
7 ) Sala C et al., Neuron (2001)
8 ) Tobaben S et al., J. Biol. Chem. (2000)
9 ) Sheng M et al., J. Cell. Sci. (2000)
10 ) Naisbitt S et al., Neuron (1999)

Protocols

ICC

IHC

IHC-P

Other products for this target

Shank2 antibody - 162 204 K.O.

References for Shank2 - 162 204