Caveolin1 antibody - 161 003

Caveolin1 is the major coat protein of caveolae

Rabbit polyclonal purified antibody

Cat. No.: 161 003

Amount: 50 µg

Price: $455.00

Cat. No. 161 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes ICC: 1 : 100 up to 1 : 500 gallery IHC: not recommended IHC-P: not tested yet IHC-Fr: 1 : 500 (see remarks) gallery IHC-G: 1 : 500 up to 1 : 1000 (see remarks) gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Immunohistochemistry on fresh frozen (IHC-Fr) cryo-tissue-sections. In contrast to standard PFA perfusion fixed tissues, fresh frozen cryo-tissue-sections can be variably postfixed with alcohols, acetone or PFA. Alcohol or acetone fixation is e.g. of advantage for antigens masked by PFA crosslinking. For recommended postfixation, please refer to the ”Remarks” section. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Immunohistochemistry on glyoxal fixed (IHC-G) tissue. The tissue is perfused with saline and afterwards immersion fixed with a glyoxal solution. For details of the glyoxal solution, please refer to the remarks section. Immunoreactivity is usually revealed by fluorescence.
Immunogen	Synthetic peptide corresponding to AA 1 to 17 from rat Caveolin1 (UniProt Id: P41350)
Reactivity	Reacts with: human (Q03135), rat (P41350), mouse (P49817), dog, pig, cow, monkey. No signal: zebrafish. Other species not tested yet.
Matching control protein/peptide	161-0P
Remarks	IHC-Fr: MeOH fixation is recommended. IHC-G: Fixation with 3% glyoxal, 1% acetic acid, 20% ethanol in ddH2O according to Richter et al. 2017, or 9% glyoxal, 8% acetic acid in ddH2O according to Konno et al. 2023 are possible.is recommended.
Data sheet	161_003.pdf

References for Caveolin1 - 161 003

Involvement of the Cdc42 pathway in CFTR post-translational turnover and in its plasma membrane stability in airway epithelial cells.
Ferru-Clément R, Fresquet F, Norez C, Métayé T, Becq F, Kitzis A, Thoreau V
PloS one (2015) 103: e0118943. 161 003 WB

Spatial proteomics in neurons at single-protein resolution.
Unterauer EM, Shetab Boushehri S, Jevdokimenko K, Masullo LA, Ganji M, Sograte-Idrissi S, Kowalewski R, Strauss S, Reinhardt SCM, Perovic A, Marr C, et al.
Cell (2024) 1877: 1785-1800.e16. 161 003 DNA-PAINT; tested species: rat

Cat. No.: 161 003

Amount: 50 µg

Price: $455.00

ICC

IHC-Fr

IHC-G

Background

Caveolae are distinct flask shaped invaginations which contain high concentrations of cholesterol and sphingolipids. These subcellular compartments can be found at the surface of many cell types.
Three isoforms (Calveolin 1, 2, 3) have been described so far. Caveolin 1 is the major coat protein of endothelial caveolae and a knock out of this protein leads to a complete loss of these cell surface structures in various cell types.
Caveolin 1 is also expressed in brain where it localizes to astrocytes, oligodendrocytes and endothelial cells but seems to be absent from neurons. Very recently a correlation of increased caveolin 1 expression in brain and Alzheimer's disease has been described.

1 ) Kang MJ et al., Exp. Mol. Med. (2006)
2 ) Gaudreault SB et al., Neurobiol. Aging (2004)
3 ) Virgintino D et al., Neuroscience (2002)
4 ) Ikezu T et al., Brain Res. (1998)
5 ) Cameron PL et al., J. Neurosci. (1997)
6 ) Rothberg KG et al., Cell (1992)

Protocols

Standard protocol

ICC

IHC-Fr

IHC-G

OTHER

Preabsorbtion

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