Caveolin1 antibody - 161 002

Caveolin1 is the major coat protein of caveolae

Rabbit polyclonal antiserum

Cat. No.: 161 002

Amount: 200 µl

Price: $355.00

Cat. No. 161 002	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes ICC: not recommended IHC: not recommended IHC-P: not tested yet IHC-Fr: not recommended Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Immunohistochemistry on fresh frozen (IHC-Fr) cryo-tissue-sections. In contrast to standard PFA perfusion fixed tissues, fresh frozen cryo-tissue-sections can be variably postfixed with alcohols, acetone or PFA. Alcohol or acetone fixation is e.g. of advantage for antigens masked by PFA crosslinking. For recommended postfixation, please refer to the ”Remarks” section. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 1 to 17 from rat Caveolin1 (UniProt Id: P41350)
Reactivity	Reacts with: human (Q03135), rat (P41350), mouse (P49817), dog, pig, cow, monkey. No signal: zebrafish. Other species not tested yet.
Matching control protein/peptide	161-0P
Data sheet	161_002.pdf

Cat. No.: 161 002

Amount: 200 µl

Price: $355.00

Background

Caveolae are distinct flask shaped invaginations which contain high concentrations of cholesterol and sphingolipids. These subcellular compartments can be found at the surface of many cell types.
Three isoforms (Calveolin 1, 2, 3) have been described so far. Caveolin 1 is the major coat protein of endothelial caveolae and a knock out of this protein leads to a complete loss of these cell surface structures in various cell types.
Caveolin 1 is also expressed in brain where it localizes to astrocytes, oligodendrocytes and endothelial cells but seems to be absent from neurons. Very recently a correlation of increased caveolin 1 expression in brain and Alzheimer's disease has been described.

1 ) Kang MJ et al., Exp. Mol. Med. (2006)
2 ) Gaudreault SB et al., Neurobiol. Aging (2004)
3 ) Virgintino D et al., Neuroscience (2002)
4 ) Ikezu T et al., Brain Res. (1998)
5 ) Cameron PL et al., J. Neurosci. (1997)
6 ) Rothberg KG et al., Cell (1992)

Protocols

Standard protocol

OTHER

Preabsorbtion

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