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Home > Products > 160 303

Homer3 antibody - 160 303

Homer1 , 2 and 3 are post synaptic density (PSD) proteins involved in the targeting of glutamate receptors
Rabbit polyclonal purified antibody
Cat. No.: 160 303
Amount: 50 µg
Price: $375.00
Cat. No. 160 303 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 250 gallery  
IHC: 1 : 500 gallery  
IHC-P: 1 : 500 gallery  
Clarity: 1 : 300 (see remarks)

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.

Clarity is a sample preparation method for clearing, immunolabeling and volume imaging of large biological samples. Clearing is carried out by SDS and Focusclear treatment. For additional experimental details, please refer to the Remarks section.
Immunogen Recombinant protein corresponding to AA 1 to 177 from rat Homer3 (UniProt Id: Q9Z2X5)
Reactivity Reacts with: rat (Q9Z2X5).
Other species not tested yet.
Specificity AA 122-177, unique for homer 3, were used for affinity purification.
Matching control protein/peptide 160-3P
Remarks

Clarity: This antibody has been successfully used for CLARITY application in human brain (Woelfle et al., 2023; PMID: 37221592).
Data sheet 160_303.pdf

References for Homer3 - 160 303

Brain-region-specific changes in neurons and glia and dysregulation of dopamine signaling in Grin2a mutant mice.
Farsi Z, Nicolella A, Simmons SK, Aryal S, Shepard N, Brenner K, Lin S, Herzog L, Moran SP, Stalnaker KJ, Shin W, et al.
Neuron (2023) 11121: 3378-3396.e9. 160 303 WB; tested species: mouse
Adult restoration of Shank3 expression rescues selective autistic-like phenotypes.
Mei Y, Monteiro P, Zhou Y, Kim JA, Gao X, Fu Z, Feng G
Nature (2016) 5307591: 481-4. 160 303 WB; tested species: mouse
Homer is concentrated at the postsynaptic density and does not redistribute after acute synaptic stimulation.
Tao-Cheng JH, Thein S, Yang Y, Reese TS, Gallant PE
Neuroscience (2014) 266: 80-90. 160 303 WB, EM
Early molecular layer interneuron hyperactivity triggers Purkinje neuron degeneration in SCA1.
Pilotto F, Douthwaite C, Diab R, Ye X, Al Qassab Z, Tietje C, Mounassir M, Odriozola A, Thapa A, Buijsen RAM, Lagache S, et al.
Neuron (2023) : . 160 303 IHC; tested species: mouse
Benchmarking miniaturized microscopy against two-photon calcium imaging using single-cell orientation tuning in mouse visual cortex.
Glas A, Hübener M, Bonhoeffer T, Goltstein PM
PloS one (2019) 144: e0214954. 160 303 IHC; tested species: mouse
CLARITY increases sensitivity and specificity of fluorescence immunostaining in long-term archived human brain tissue.
Woelfle S, Deshpande D, Feldengut S, Braak H, Del Tredici K, Roselli F, Deisseroth K, Michaelis J, Boeckers TM, Schön M
BMC biology (2023) 211: 113. 160 303 Clarity; tested species: human
Homer is concentrated at the postsynaptic density and does not redistribute after acute synaptic stimulation.
Tao-Cheng JH, Thein S, Yang Y, Reese TS, Gallant PE
Neuroscience (2014) 266: 80-90. 160 303 WB, EM
Cat. No.: 160 303
Amount: 50 µg
Price: $375.00
Brain-region-specific changes in neurons and glia and dysregulation of dopamine signaling in Grin2a mutant mice.
Farsi Z, Nicolella A, Simmons SK, Aryal S, Shepard N, Brenner K, Lin S, Herzog L, Moran SP, Stalnaker KJ, Shin W, et al.
Neuron (2023) 11121: 3378-3396.e9. 160 303 WB; tested species: mouse
Adult restoration of Shank3 expression rescues selective autistic-like phenotypes.
Mei Y, Monteiro P, Zhou Y, Kim JA, Gao X, Fu Z, Feng G
Nature (2016) 5307591: 481-4. 160 303 WB; tested species: mouse
Homer is concentrated at the postsynaptic density and does not redistribute after acute synaptic stimulation.
Tao-Cheng JH, Thein S, Yang Y, Reese TS, Gallant PE
Neuroscience (2014) 266: 80-90. 160 303 WB, EM
Early molecular layer interneuron hyperactivity triggers Purkinje neuron degeneration in SCA1.
Pilotto F, Douthwaite C, Diab R, Ye X, Al Qassab Z, Tietje C, Mounassir M, Odriozola A, Thapa A, Buijsen RAM, Lagache S, et al.
Neuron (2023) : . 160 303 IHC; tested species: mouse
Benchmarking miniaturized microscopy against two-photon calcium imaging using single-cell orientation tuning in mouse visual cortex.
Glas A, Hübener M, Bonhoeffer T, Goltstein PM
PloS one (2019) 144: e0214954. 160 303 IHC; tested species: mouse
CLARITY increases sensitivity and specificity of fluorescence immunostaining in long-term archived human brain tissue.
Woelfle S, Deshpande D, Feldengut S, Braak H, Del Tredici K, Roselli F, Deisseroth K, Michaelis J, Boeckers TM, Schön M
BMC biology (2023) 211: 113. 160 303 Clarity; tested species: human
Homer is concentrated at the postsynaptic density and does not redistribute after acute synaptic stimulation.
Tao-Cheng JH, Thein S, Yang Y, Reese TS, Gallant PE
Neuroscience (2014) 266: 80-90. 160 303 WB, EM
WB
ICC
IHC
IHC-P
Background

Homer is a scaffolding protein of the post synaptic density (PSD) and enriched at excitatory synapses. The protein binds metabotropic glutamate receptors, TRPC1, proteins of the Shank family and others. By aggregating these proteins into clusters, Homer was suggested to organize distinct signalling domains.
Three isoforms, Homer 1, 2 and 3 have been described. Each of these isoforms is subject to alternative splicing yielding the splice variants a, b, c, d.
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