Homer1b/c antibody - 160 111

Homer1, 2 and 3 are post synaptic density (PSD) proteins involved in the targeting of glutamate receptors

Mouse monoclonal purified IgG

Cat. No.: 160 111

Amount: 100 µg

Price: $415.00

Cat. No. 160 111	100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes ICC: 1 : 500 gallery IHC: 1 : 100 up to 1 : 500 (see remarks) gallery IHC-P: 1 : 200 up to 1 : 1000 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	SY-72G2
Subtype	IgG1 (κ light chain)
Immunogen	Recombinant protein corresponding to the c-terminal half of human Homer1b (UniProt Id: Q86YM7-1)
Reactivity	Reacts with: mouse (Q9Z2Y3), rat (Q9Z214), human (Q86YM7-1). Other species not tested yet.
Specificity	Specific for Homer1b and 1c; no cross-reactivity to Homer1a.
Remarks	IHC: Heat-mediated antigen retrieval (in citrate buffer pH 6) can be applied to increase signal strength.
Data sheet	160_111.pdf

References for Homer1b/c - 160 111

Hyperfunction of post-synaptic density protein 95 promotes seizure response in early-stage aβ pathology.
Yook Y, Lee KY, Kim E, Lizarazo S, Yu X, Tsai NP
EMBO reports (2024) : . 160 111 ICC; tested species: mouse

Cat. No.: 160 111

Amount: 100 µg

Price: $415.00

ICC

IHC

IHC-P

Background

Homer is a scaffolding protein of the post synaptic density (PSD) and enriched at excitatory synapses. The protein binds metabotropic glutamate receptors, TRPC1, proteins of the Shank family and others. By aggregating these proteins into clusters, homer was suggested to organize distinct signalling domains.
Three isoforms, Homer1, 2 and 3 have been described. Each of these isoforms is subject to alternative splicing yielding the splice variants a, b, c, d.

1 ) Kammermeier PJ et al., BMC Neurosci (2006)
2 ) Yamamoto K et al., Eur. J. Neurosci. (2005)
3 ) Thomas U et al., J. Neurochem. (2002)
4 ) Ango F et al., Mol. Cell. Neurosci. (2002)
5 ) Sala C et al., Neuron (2001)
6 ) Ciruela F et al., Mol. Cell. Neurosci. (2000)
7 ) Xiao B et al., Curr. Opin. Neurobiol. (2000)
8 ) Tu JC et al., Neuron (1999)
9 ) Brakeman PR et al., Nature (1997)
10 ) Shiraishi Y et al., J. Comp. Neurol. (2004)

Protocols

Standard protocol

ICC

IHC

IHC-P

Other products for this target