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Home > Products > 160 103

Homer1/2/3 antibody - 160 103

Homer1 , 2 and 3 are post synaptic density (PSD) proteins involved in the targeting of glutamate receptors
Rabbit polyclonal purified antibody
Cat. No.: 160 103
Amount: 50 µg
Price: $385.00
Cat. No. 160 103 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 200 up to 1 : 500 (see remarks) gallery  
IHC: 1 : 500 gallery  
IHC-P: 1 : 500 gallery  
EM: yes

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.

Electron microscopy (EM) is a microscopy technique that detects the scatter of electrons through thin tissue sections. In Immuno-EM the antigen is usually revealed by colloidal gold conjugated secondary antibodies linking electron dense structures to antigen-bound primary antibodies.
Immunogen Mix of recombinant proteins corresponding to AA 1 to 196 from human Homer 1, AA 1 to 176 from rat Homer 2 and AA 1 to 177 from rat Homer 3
Reactivity Reacts with: human (Q86YM7, Q9NSB8, Q9NSC5), rat (Q9Z214, O88801, Q9Z2X5), mouse (Q9Z2Y3, Q9QWW1, Q99JP6), zebrafish.
Other species not tested yet.
Specificity AA 1-120 were used for affinity purification which are homologous in homer 1, 2, and 3.
Remarks

ICC: PFA fixation recommended.
Data sheet 160_103.pdf

References for Homer1/2/3 - 160 103

Evolving prion-like tau conformers differentially alter postsynaptic proteins in neurons inoculated with distinct isolates of Alzheimer's disease tau.
Hromadkova L, Kim C, Haldiman T, Peng L, Zhu X, Cohen M, de Silva R, Safar JG
Cell & bioscience (2023) 131: 174. 160 103 WB, ICC; tested species: mouse
Truncating variants in the SHANK1 gene are associated with a spectrum of neurodevelopmental disorders.
May HJ, Jeong J, Revah-Politi A, Cohen JS, Chassevent A, Baptista J, Baugh EH, Bier L, Bottani A, Carminho A Rodrigues MT, Conlon C, et al.
Genetics in medicine : official journal of the American College of Medical Genetics (2021) 2310: 1912-1921. 160 103 WB; tested species: mouse
Postsynaptic densities fragment into subcomplexes upon sonication.
Dosemeci A, Tao-Cheng JH, Bakly V, Reese TS
Molecular brain (2019) 121: 72. 160 103 WB; tested species: rat
Homer is concentrated at the postsynaptic density and does not redistribute after acute synaptic stimulation.
Tao-Cheng JH, Thein S, Yang Y, Reese TS, Gallant PE
Neuroscience (2014) 266: 80-90. 160 103 WB, EM
Metabotropic glutamate receptor 5/Homer interactions underlie stress effects on fear.
Tronson NC, Guzman YF, Guedea AL, Huh KH, Gao C, Schwarz MK, Radulovic J
Biological psychiatry (2010) 6811: 1007-15. 160 103 WB, IHC
Evolving prion-like tau conformers differentially alter postsynaptic proteins in neurons inoculated with distinct isolates of Alzheimer's disease tau.
Hromadkova L, Kim C, Haldiman T, Peng L, Zhu X, Cohen M, de Silva R, Safar JG
Cell & bioscience (2023) 131: 174. 160 103 WB, ICC; tested species: mouse
Autism-associated SHANK3 missense point mutations impact conformational fluctuations and protein turnover at synapses.
Bucher M, Niebling S, Han Y, Molodenskiy D, Hassani Nia F, Kreienkamp HJ, Svergun D, Kim E, Kostyukova AS, Kreutz MR, Mikhaylova M, et al.
eLife (2021) 10: . 160 103 ICC; tested species: rat
Growth-Promoting Treatment Screening for Corticospinal Neurons in Mouse and Man.
Hanuscheck N, Schnatz A, Thalman C, Lerch S, Gärtner Y, Domingues M, Bitar L, Nitsch R, Zipp F, Vogelaar CF
Cellular and molecular neurobiology (2020) : . 160 103 ICC; tested species: human
Thrombospondin-4 and excitatory synaptogenesis promote spinal sensitization after painful mechanical joint injury.
Crosby ND, Zaucke F, Kras JV, Dong L, Luo ZD, Winkelstein BA
Experimental neurology (2015) 264: 111-20. 160 103 ICC
Visualizing Synaptic Multi-Protein Patterns of Neuronal Tissue With DNA-Assisted Single-Molecule Localization Microscopy.
Narayanasamy KK, Stojic A, Li Y, Sass S, Hesse MR, Deussner-Helfmann NS, Dietz MS, Kuner T, Klevanski M, Heilemann M
Frontiers in synaptic neuroscience (2021) 13: 671288. 160 103 IHC; tested species: rat
Volumetric super-resolution imaging by serial ultrasectioning and stochastic optical reconstruction microscopy in mouse neural tissue.
Vatan T, Minehart JA, Zhang C, Agarwal V, Yang J, Speer CM
STAR protocols (2021) 24: 100971. 160 103 IHC; tested species: mouse
Automated highly multiplexed super-resolution imaging of protein nano-architecture in cells and tissues.
Klevanski M, Herrmannsdoerfer F, Sass S, Venkataramani V, Heilemann M, Kuner T
Nature communications (2020) 111: 1552. 160 103 IHC; tested species: rat
Volume gradients in inner hair cell-auditory nerve fiber pre- and postsynaptic proteins differ across mouse strains.
Reijntjes DOJ, Köppl C, Pyott SJ
Hearing research (2020) 390: 107933. 160 103 IHC; tested species: mouse
Metabotropic glutamate receptor 5/Homer interactions underlie stress effects on fear.
Tronson NC, Guzman YF, Guedea AL, Huh KH, Gao C, Schwarz MK, Radulovic J
Biological psychiatry (2010) 6811: 1007-15. 160 103 WB, IHC
Homer is concentrated at the postsynaptic density and does not redistribute after acute synaptic stimulation.
Tao-Cheng JH, Thein S, Yang Y, Reese TS, Gallant PE
Neuroscience (2014) 266: 80-90. 160 103 WB, EM
Cat. No.: 160 103
Amount: 50 µg
Price: $385.00
Evolving prion-like tau conformers differentially alter postsynaptic proteins in neurons inoculated with distinct isolates of Alzheimer's disease tau.
Hromadkova L, Kim C, Haldiman T, Peng L, Zhu X, Cohen M, de Silva R, Safar JG
Cell & bioscience (2023) 131: 174. 160 103 WB, ICC; tested species: mouse
Truncating variants in the SHANK1 gene are associated with a spectrum of neurodevelopmental disorders.
May HJ, Jeong J, Revah-Politi A, Cohen JS, Chassevent A, Baptista J, Baugh EH, Bier L, Bottani A, Carminho A Rodrigues MT, Conlon C, et al.
Genetics in medicine : official journal of the American College of Medical Genetics (2021) 2310: 1912-1921. 160 103 WB; tested species: mouse
Postsynaptic densities fragment into subcomplexes upon sonication.
Dosemeci A, Tao-Cheng JH, Bakly V, Reese TS
Molecular brain (2019) 121: 72. 160 103 WB; tested species: rat
Homer is concentrated at the postsynaptic density and does not redistribute after acute synaptic stimulation.
Tao-Cheng JH, Thein S, Yang Y, Reese TS, Gallant PE
Neuroscience (2014) 266: 80-90. 160 103 WB, EM
Metabotropic glutamate receptor 5/Homer interactions underlie stress effects on fear.
Tronson NC, Guzman YF, Guedea AL, Huh KH, Gao C, Schwarz MK, Radulovic J
Biological psychiatry (2010) 6811: 1007-15. 160 103 WB, IHC
Evolving prion-like tau conformers differentially alter postsynaptic proteins in neurons inoculated with distinct isolates of Alzheimer's disease tau.
Hromadkova L, Kim C, Haldiman T, Peng L, Zhu X, Cohen M, de Silva R, Safar JG
Cell & bioscience (2023) 131: 174. 160 103 WB, ICC; tested species: mouse
Autism-associated SHANK3 missense point mutations impact conformational fluctuations and protein turnover at synapses.
Bucher M, Niebling S, Han Y, Molodenskiy D, Hassani Nia F, Kreienkamp HJ, Svergun D, Kim E, Kostyukova AS, Kreutz MR, Mikhaylova M, et al.
eLife (2021) 10: . 160 103 ICC; tested species: rat
Growth-Promoting Treatment Screening for Corticospinal Neurons in Mouse and Man.
Hanuscheck N, Schnatz A, Thalman C, Lerch S, Gärtner Y, Domingues M, Bitar L, Nitsch R, Zipp F, Vogelaar CF
Cellular and molecular neurobiology (2020) : . 160 103 ICC; tested species: human
Thrombospondin-4 and excitatory synaptogenesis promote spinal sensitization after painful mechanical joint injury.
Crosby ND, Zaucke F, Kras JV, Dong L, Luo ZD, Winkelstein BA
Experimental neurology (2015) 264: 111-20. 160 103 ICC
Visualizing Synaptic Multi-Protein Patterns of Neuronal Tissue With DNA-Assisted Single-Molecule Localization Microscopy.
Narayanasamy KK, Stojic A, Li Y, Sass S, Hesse MR, Deussner-Helfmann NS, Dietz MS, Kuner T, Klevanski M, Heilemann M
Frontiers in synaptic neuroscience (2021) 13: 671288. 160 103 IHC; tested species: rat
Volumetric super-resolution imaging by serial ultrasectioning and stochastic optical reconstruction microscopy in mouse neural tissue.
Vatan T, Minehart JA, Zhang C, Agarwal V, Yang J, Speer CM
STAR protocols (2021) 24: 100971. 160 103 IHC; tested species: mouse
Automated highly multiplexed super-resolution imaging of protein nano-architecture in cells and tissues.
Klevanski M, Herrmannsdoerfer F, Sass S, Venkataramani V, Heilemann M, Kuner T
Nature communications (2020) 111: 1552. 160 103 IHC; tested species: rat
Volume gradients in inner hair cell-auditory nerve fiber pre- and postsynaptic proteins differ across mouse strains.
Reijntjes DOJ, Köppl C, Pyott SJ
Hearing research (2020) 390: 107933. 160 103 IHC; tested species: mouse
Metabotropic glutamate receptor 5/Homer interactions underlie stress effects on fear.
Tronson NC, Guzman YF, Guedea AL, Huh KH, Gao C, Schwarz MK, Radulovic J
Biological psychiatry (2010) 6811: 1007-15. 160 103 WB, IHC
Homer is concentrated at the postsynaptic density and does not redistribute after acute synaptic stimulation.
Tao-Cheng JH, Thein S, Yang Y, Reese TS, Gallant PE
Neuroscience (2014) 266: 80-90. 160 103 WB, EM
WB
ICC
IHC
IHC-P
Background

Homer is a scaffolding protein of the post synaptic density (PSD) and enriched at excitatory synapses. The protein binds metabotropic glutamate receptors, TRPC1, proteins of the Shank family and others. By aggregating these proteins into clusters, Homer was suggested to organize distinct signalling domains.
Three isoforms, Homer 1, 2 and 3 have been described. Each of these isoforms is subject to alternative splicing yielding the splice variants a, b, c, d.