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Homer 1/2/3 - 160 103

A post synaptic density (PSD) protein involved in the targeting of glutamate receptors
Polyclonal rabbit purified antibody
Cat. No.: 160 103
Amount: 50 µg
Price: $370.00
Cat. No. 160 103 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Applications WB: 1 : 1000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 200 up to 1 : 500 (see remarks) gallery  
IHC: 1 : 500 gallery  
IHC-P/FFPE: 1 : 500 gallery  
EM: yes
Immunogen Mix of recombinant proteins corresponding to AA 1 to 196 from human Homer 1, AA 1 to 176 from rat Homer 2 and AA 1 to 177 from rat Homer 3
Reactivity Reacts with: human (Q86YM7, Q9NSB8, Q9NSC5), rat (Q9Z214, O88801, Q9Z2X5), mouse (Q9Z2Y3, Q9QWW1, Q99JP6), zebrafish.
Other species not tested yet.
Specificity AA 1-120 were used for affinity purification which are homologous in homer 1, 2, and 3.
Remarks

ICC: PFA fixation recommended.

Data sheet 160_103.pdf

References for Homer 1/2/3 - 160 103

Postsynaptic densities fragment into subcomplexes upon sonication.
Dosemeci A, Tao-Cheng JH, Bakly V, Reese TS
Molecular brain (2019) 121: 72. 160 103 WB; tested species: rat
Homer is concentrated at the postsynaptic density and does not redistribute after acute synaptic stimulation.
Tao-Cheng JH, Thein S, Yang Y, Reese TS, Gallant PE
Neuroscience (2014) 266: 80-90. 160 103 WB, EM
Metabotropic glutamate receptor 5/Homer interactions underlie stress effects on fear.
Tronson NC, Guzman YF, Guedea AL, Huh KH, Gao C, Schwarz MK, Radulovic J
Biological psychiatry (2010) 6811: 1007-15. 160 103 WB, IHC
Growth-Promoting Treatment Screening for Corticospinal Neurons in Mouse and Man.
Hanuscheck N, Schnatz A, Thalman C, Lerch S, Gärtner Y, Domingues M, Bitar L, Nitsch R, Zipp F, Vogelaar CF
Cellular and molecular neurobiology (2020) : . 160 103 ICC; tested species: human
Thrombospondin-4 and excitatory synaptogenesis promote spinal sensitization after painful mechanical joint injury.
Crosby ND, Zaucke F, Kras JV, Dong L, Luo ZD, Winkelstein BA
Experimental neurology (2015) 264: 111-20. 160 103 ICC
Automated highly multiplexed super-resolution imaging of protein nano-architecture in cells and tissues.
Klevanski M, Herrmannsdoerfer F, Sass S, Venkataramani V, Heilemann M, Kuner T
Nature communications (2020) 111: 1552. 160 103 IHC; tested species: rat
Volume gradients in inner hair cell-auditory nerve fiber pre- and postsynaptic proteins differ across mouse strains.
Reijntjes DOJ, Köppl C, Pyott SJ
Hearing research (2020) 390: 107933. 160 103 IHC; tested species: mouse
Metabotropic glutamate receptor 5/Homer interactions underlie stress effects on fear.
Tronson NC, Guzman YF, Guedea AL, Huh KH, Gao C, Schwarz MK, Radulovic J
Biological psychiatry (2010) 6811: 1007-15. 160 103 WB, IHC
Homer is concentrated at the postsynaptic density and does not redistribute after acute synaptic stimulation.
Tao-Cheng JH, Thein S, Yang Y, Reese TS, Gallant PE
Neuroscience (2014) 266: 80-90. 160 103 WB, EM
Cat. No.: 160 103
Quantity: 50 µg
Price: $370.00
Postsynaptic densities fragment into subcomplexes upon sonication.
Dosemeci A, Tao-Cheng JH, Bakly V, Reese TS
Molecular brain (2019) 121: 72. 160 103 WB; tested species: rat
Homer is concentrated at the postsynaptic density and does not redistribute after acute synaptic stimulation.
Tao-Cheng JH, Thein S, Yang Y, Reese TS, Gallant PE
Neuroscience (2014) 266: 80-90. 160 103 WB, EM
Metabotropic glutamate receptor 5/Homer interactions underlie stress effects on fear.
Tronson NC, Guzman YF, Guedea AL, Huh KH, Gao C, Schwarz MK, Radulovic J
Biological psychiatry (2010) 6811: 1007-15. 160 103 WB, IHC
Growth-Promoting Treatment Screening for Corticospinal Neurons in Mouse and Man.
Hanuscheck N, Schnatz A, Thalman C, Lerch S, Gärtner Y, Domingues M, Bitar L, Nitsch R, Zipp F, Vogelaar CF
Cellular and molecular neurobiology (2020) : . 160 103 ICC; tested species: human
Thrombospondin-4 and excitatory synaptogenesis promote spinal sensitization after painful mechanical joint injury.
Crosby ND, Zaucke F, Kras JV, Dong L, Luo ZD, Winkelstein BA
Experimental neurology (2015) 264: 111-20. 160 103 ICC
Automated highly multiplexed super-resolution imaging of protein nano-architecture in cells and tissues.
Klevanski M, Herrmannsdoerfer F, Sass S, Venkataramani V, Heilemann M, Kuner T
Nature communications (2020) 111: 1552. 160 103 IHC; tested species: rat
Volume gradients in inner hair cell-auditory nerve fiber pre- and postsynaptic proteins differ across mouse strains.
Reijntjes DOJ, Köppl C, Pyott SJ
Hearing research (2020) 390: 107933. 160 103 IHC; tested species: mouse
Metabotropic glutamate receptor 5/Homer interactions underlie stress effects on fear.
Tronson NC, Guzman YF, Guedea AL, Huh KH, Gao C, Schwarz MK, Radulovic J
Biological psychiatry (2010) 6811: 1007-15. 160 103 WB, IHC
Homer is concentrated at the postsynaptic density and does not redistribute after acute synaptic stimulation.
Tao-Cheng JH, Thein S, Yang Y, Reese TS, Gallant PE
Neuroscience (2014) 266: 80-90. 160 103 WB, EM
Background

Homer is a scaffolding protein of the post synaptic density (PSD) and enriched at excitatory synapses. The protein binds metabotropic glutamate receptors, TRPC1, proteins of the Shank family and others. By aggregating these proteins into clusters, Homer was suggested to organize distinct signalling domains.
Three isoforms, Homer 1, 2 and 3 have been described. Each of these isoforms is subject to alternative splicing yielding the splice variants a, b, c, d.