Homer1b/c antibody - 160 026

Homer1 , 2 and 3 are post synaptic density (PSD) proteins involved in the targeting of glutamate receptors

Chicken polyclonal IgY fraction

Cat. No.: 160 026

Amount: 200 µl

Price: $360.00

For the import of chicken antibodies into the USA you need a special import permit. This can be applied for at your USDA office. If you cannot apply for this, please order the chicken antibodies through our distributor Cedarlane USA. If you have any questions, please contact us.

Cat. No. 160 026	200 µl antibody, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 500 up to 1 : 1000 (AP staining) gallery IP: not tested yet ICC: 1 : 500 up to 1 : 1000 gallery IHC: 1 : 500 up to 1 : 1000 gallery IHC-P: 1 : 500 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to the c-terminal half of human Homer 1b (UniProt Id: Q86YM7-1)
Reactivity	Reacts with: mouse (Q9Z2Y3-3, Q9Z2Y3-2), rat (Q9Z214-2, Q9Z214-1), human (Q86YM7-1, Q86YM7-2). Other species not tested yet.
Specificity	Specific for homer 1b and 1c; no cross-reactivity to homer 1a.
Data sheet	160_026.pdf

Cat. No.: 160 026

Amount: 200 µl

Price: $360.00

ICC

IHC

IHC-P

Background

Homer is a scaffolding protein of the post synaptic density (PSD) and enriched at excitatory synapses. The protein binds metabotropic glutamate receptors, TRPC1, proteins of the Shank family and others. By aggregating these proteins into clusters, homer was suggested to organize distinct signalling domains.
Three isoforms, Homer1, 2 and 3 have been described. Each of these isoforms is subject to alternative splicing yielding the splice variants a, b, c, d.

1 ) Kammermeier PJ et al., BMC Neurosci (2006)
2 ) Yamamoto K et al., Eur. J. Neurosci. (2005)
3 ) Thomas U et al., J. Neurochem. (2002)
4 ) Ango F et al., Mol. Cell. Neurosci. (2002)
5 ) Sala C et al., Neuron (2001)
6 ) Ciruela F et al., Mol. Cell. Neurosci. (2000)
7 ) Xiao B et al., Curr. Opin. Neurobiol. (2000)
8 ) Tu JC et al., Neuron (1999)
9 ) Brakeman PR et al., Nature (1997)
10 ) Shiraishi Y et al., J. Comp. Neurol. (2004)

Protocols

ICC

IHC

IHC-P

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