|Cat. No. 159 103||50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.|
WB: 1 : 1000 up to 1 : 5000 AP staining gallery
IP: not tested yet
ICC: 1 : 500 gallery
IHC: 1 : 500 gallery
IHC-P/FFPE: not tested yet
|Immunogen||Synthetic peptide corresponding to AA 256 to 270 from mouse Endophilin2 (UniProt Id: Q62419)|
Reacts with: mouse (Q62419), rat (O35964).
Other species not tested yet.
|Specificity||Specific for Endophilin 2|
Endophilins are SH3 domain proteins involved in endocytosis. Both, Endophilin 1 and 2 have been shown to play important roles in clathrin mediated synaptic vesicle recycling. They recruite and stabilize the polyphosphoinositide phosphatase synaptojanin at nerve terminals.
The divergent C-terminal tail of VgluT1 has been reported to be a binding partner of Endophilin A1.
In contrast to Endophilin 1 that shows a brain specific expression, Endophilin 2 is abundantly expressed in different tissues.