Endophilin2 antibody - 159 103

Endophilin2 is an SH3 domain protein involved in clathrin mediated endocytosis

Rabbit polyclonal purified antibody

Cat. No.: 159 103

Amount: 50 µg

Price: $380.00

Cat. No. 159 103	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 up to 1 : 5000 (AP staining) gallery IP: not tested yet ICC: 1 : 500 gallery IHC: 1 : 500 gallery IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 256 to 270 from mouse Endophilin2 (UniProt Id: Q62419)
Reactivity	Reacts with: mouse (Q62419), rat (O35964). Other species not tested yet.
Data sheet	Datasheet 159_103

Cat. No.: 159 103

Amount: 50 µg

Price: $380.00

ICC

IHC

Background

Endophilins are SH3 domain proteins involved in endocytosis. Both, Endophilin 1 and 2 have been shown to play important roles in clathrin mediated synaptic vesicle recycling. They recruite and stabilize the polyphosphoinositide phosphatase synaptojanin at nerve terminals.

The divergent C-terminal tail of VgluT1 has been reported to be a binding partner of Endophilin A1.

In contrast to Endophilin 1 that shows a brain specific expression, Endophilin 2 is abundantly expressed in different tissues.

1 ) Vinatier J et al., J. Neurochem. (2006)
2 ) Schuske KR et al., Neuron (2003)
3 ) Verstreken P et al., Neuron (2003)
4 ) Song W et al., Neuron (2003)
5 ) Chen Y et al., Cell (2003)
6 ) Reutens AT et al., Int. J. Biochem. Cell Biol. (2002)
7 ) Verstreken P et al., Cell (2002)
8 ) Gad H et al., Neuron (2000)
9 ) Ringstad N et al., Neuron (1999)
10 ) Schmidt A et al., Nature (1999)

Protocols

ICC

Fixed Cells

IHC