Arc antibody - 156 111

Arc is a cytoskeleton-associated protein involved in neuronal plasticity

Mouse monoclonal purified IgG

Cat. No.: 156 111

Amount: 100 µg

Price: $415.00

Cat. No. 156 111	100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: not tested yet IHC: 1 : 500 gallery IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	73A5
Subtype	IgG1 (κ light chain)
Immunogen	Synthetic peptide corresponding to residues near the carboxy terminus of mouse Arc (UniProt Id: Q9WV31)
Reactivity	Reacts with: mouse (Q9WV31), rat (Q63053). Other species not tested yet.
Specificity	Specific for Arc.
Data sheet	156_111.pdf

Cat. No.: 156 111

Amount: 100 µg

Price: $415.00

IHC

Background

Immediate-early genes (IEGs) are rapidely induced after patterned synaptic activity. Genes that are involved in this complex response code for transcription and growth factors, metabolic and signaling enzymes, small GTP binding proteins and structural proteins. Some of these proteins may play a crucial role in long term plasticity which is important for learning processes.
The activity regulated cytoskeleton associated protein Arc or Arg 3.1 is enriched in dendrites and colocalizes with F-Actin. Direct interaction of Arc with actin has also been demonstrated by biochemical studies.

1 ) Larsen MH et al., Brain Res. (2005)
2 ) McIntyre CK et al., Proc. Natl. Acad. Sci. U.S.A. (2005)
3 ) Chawla MK et al., Hippocampus (2005)
4 ) Vazdarjanova A et al., J. Neurosci. (2002)
5 ) Guzowski JF et al., J. Neurosci. (2000)
6 ) Tan A et al., J. Neurochem. (2000)
7 ) Guzowski JF et al., Nat. Neurosci. (1999)
8 ) Steward O et al., Neuron (1998)
9 ) Lyford GL et al., Neuron (1995)

Protocols

IHC

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